6j0h

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'''Unreleased structure'''
 
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The entry 6j0h is ON HOLD
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==Crystal structure of Actinomycin D- d(TTGGCGAA) complex==
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<StructureSection load='6j0h' size='340' side='right'caption='[[6j0h]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6j0h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J0H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.52&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DVA:D-VALINE'>DVA</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PXZ:2-AMINO-1,9-DICARBONYL-4,6-DIMETHYL-10-DEHYDRO-PHENOXAZIN-3-ONE'>PXZ</scene>, <scene name='pdbligand=SAR:SARCOSINE'>SAR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j0h OCA], [https://pdbe.org/6j0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j0h RCSB], [https://www.ebi.ac.uk/pdbsum/6j0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j0h ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA mismatches are highly polymorphic and dynamic in nature, albeit poorly characterized structurally. We utilized the antitumour antibiotic CoII(Chro)2 (Chro = chromomycin A3) to stabilize the palindromic duplex d(TTGGCGAA) DNA with two G:G mismatches, allowing X-ray crystallography-based monitoring of mismatch polymorphism. For the first time, the unusual geometry of several G:G mismatches including syn-syn, water mediated anti-syn and syn-syn-like conformations can be simultaneously observed in the crystal structure. The G:G mismatch sites of the d(TTGGCGAA) duplex can also act as a hotspot for the formation of alternative DNA structures with a GC/GA-5' intercalation site for binding by the GC-selective intercalator actinomycin D (ActiD). Direct intercalation of two ActiD molecules to G:G mismatch sites causes DNA rearrangements, resulting in backbone distortion to form right-handed Z-DNA structures with a single-step sharp kink. Our study provides insights on intercalators-mismatch DNA interactions and a rationale for mismatch interrogation and detection via DNA intercalation.
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Authors: Satange, R.B., Hou, M.H.
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Polymorphic G:G mismatches act as hotspots for inducing right-handed Z DNA by DNA intercalation.,Satange R, Chuang CY, Neidle S, Hou MH Nucleic Acids Res. 2019 Jul 30. pii: 5541096. doi: 10.1093/nar/gkz653. PMID:31361900<ref>PMID:31361900</ref>
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Description: Crystal structure of Actinomycin D-d(TTGGCGAA) complex
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Hou, M.H]]
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<div class="pdbe-citations 6j0h" style="background-color:#fffaf0;"></div>
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[[Category: Satange, R.B]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptomyces sp]]
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[[Category: Synthetic construct]]
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[[Category: Hou MH]]
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[[Category: Satange RB]]

Current revision

Crystal structure of Actinomycin D- d(TTGGCGAA) complex

PDB ID 6j0h

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