6n3e

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==Structure of HIV Tat-specific factor 1 U2AF Homology Motif bound to U2AF ligand motif 4==
==Structure of HIV Tat-specific factor 1 U2AF Homology Motif bound to U2AF ligand motif 4==
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<StructureSection load='6n3e' size='340' side='right' caption='[[6n3e]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
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<StructureSection load='6n3e' size='340' side='right'caption='[[6n3e]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6n3e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N3E FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6n3e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N3E FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.893&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n3e OCA], [http://pdbe.org/6n3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n3e RCSB], [http://www.ebi.ac.uk/pdbsum/6n3e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n3e ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n3e OCA], [https://pdbe.org/6n3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n3e RCSB], [https://www.ebi.ac.uk/pdbsum/6n3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n3e ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/HTSF1_HUMAN HTSF1_HUMAN]] Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.<ref>PMID:10393184</ref> <ref>PMID:10454543</ref> <ref>PMID:10913173</ref> <ref>PMID:11420046</ref> <ref>PMID:11780068</ref> <ref>PMID:15905670</ref> <ref>PMID:8849451</ref> <ref>PMID:9765201</ref>
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[https://www.uniprot.org/uniprot/HTSF1_HUMAN HTSF1_HUMAN] Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.<ref>PMID:10393184</ref> <ref>PMID:10454543</ref> <ref>PMID:10913173</ref> <ref>PMID:11420046</ref> <ref>PMID:11780068</ref> <ref>PMID:15905670</ref> <ref>PMID:8849451</ref> <ref>PMID:9765201</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The transcription elongation and pre-mRNA splicing factor Tat-SF1 associates with the U2 small nuclear ribonucleoprotein (snRNP) of the spliceosome. However, the direct binding partner and underlying interactions mediating the Tat-SF1-U2 snRNP association remain unknown. Here, we identified SF3b1 as a Tat-SF1-interacting subunit of the U2 snRNP. Our 1.1 A resolution crystal structure revealed that Tat-SF1 contains a U2AF homology motif (UHM) protein-protein interaction module. We demonstrated that Tat-SF1 preferentially and directly binds the SF3b1 subunit compared to other U2AF ligand motif (ULM)-containing splicing factors, and further established that SF3b1 association depends on the integrity of the Tat-SF1 UHM. We next compared the Tat-SF1 binding affinities for each of the five known SF3b1 ULMs and then determined the structures of representative high- and low-affinity SF3b1 ULM complexes with the Tat-SF1 UHM at 1.9 A and 2.1 A resolutions, respectively. These structures revealed a canonical UHM-ULM interface, comprising a Tat-SF1 binding pocket for a ULM tryptophan (SF3b1 W338) and electrostatic interactions with a basic ULM tail. Importantly, we found that SF3b1 regulates Tat-SF1 levels and that these two factors influence expression of overlapping representative transcripts, consistent with a functional partnership of Tat-SF1 and SF3b1. Altogether, these results define a new molecular interface of the Tat-SF1-U2 snRNP complex for gene regulation.
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The pre-mRNA splicing and transcription factor Tat-SF1 is a functional partner of the spliceosome SF3b1 subunit via a U2AF homology motif interface.,Loerch S, Leach JR, Horner SW, Maji D, Jenkins JL, Pulvino M, Kielkopf CL J Biol Chem. 2018 Dec 19. pii: RA118.006764. doi: 10.1074/jbc.RA118.006764. PMID:30567737<ref>PMID:30567737</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6n3e" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Jenkins, J L]]
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[[Category: Homo sapiens]]
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[[Category: Kielkopf, C L]]
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[[Category: Large Structures]]
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[[Category: Loerch, S]]
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[[Category: Jenkins JL]]
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[[Category: Hiv tat-specific factor 1]]
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[[Category: Kielkopf CL]]
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[[Category: Pre-mrna splicing factor]]
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[[Category: Loerch S]]
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[[Category: Protein binding]]
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[[Category: Protein-peptide complex]]
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[[Category: Rna binding protein]]
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[[Category: Rna splicing factor]]
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[[Category: U2af homology motif]]
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[[Category: U2af ligand motif]]
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[[Category: Uhm]]
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[[Category: Ulm]]
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Current revision

Structure of HIV Tat-specific factor 1 U2AF Homology Motif bound to U2AF ligand motif 4

PDB ID 6n3e

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