3l41

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of S. pombe Brc1 BRCT5-BRCT6 domains in complex with phosphorylated H2A

Structural highlights

3l41 is a 2 chain structure with sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.45Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BRC1_SCHPO Required for mitotic fidelity, specifically in the G2 phase of the cell cycle. Plays a role in chromatin organization.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

ATM(Tel1) and ATR(Rad3) checkpoint kinases phosphorylate the C-terminus of histone H2AX (H2A in yeasts) in chromatin flanking DNA damage, establishing a recruitment platform for checkpoint and repair proteins. Phospho-H2A/X (gammaH2A/X)-binding proteins at double-strand breaks (DSBs) have been characterized, but those required for replication stress responses are unknown. Here, we present genetic, biochemical, small angle X-ray scattering (SAXS), and X-ray structural studies of the Schizosaccharomyces pombe Brc1, a 6-BRCT-domain protein that is structurally related to Saccharomyces cerevisiae Rtt107 and mammalian PTIP. Brc1 binds gammaH2A to form spontaneous and DNA damage-induced nuclear foci. Spontaneous Brc1 foci colocalize with ribosomal DNA repeats, a region prone to fork pausing and genomic instability, whereas DNA damage-induced Brc1 foci colocalize with DSB response factors. gammaH2A binding is critical for Brc1 function. The 1.45 A resolution crystal structure of Brc1-gammaH2A complex shows how variable BRCT insertion loops sculpt tandem-BRCT phosphoprotein-binding pockets to facilitate unique phosphoprotein-interaction specificities, and unveils an acidic DNA-mimicking Brc1 surface. From these results, Brc1 docking to gammaH2A emerges as a critical chromatin-specific response to replication-associated DNA damage.

gammaH2A binds Brc1 to maintain genome integrity during S-phase.,Williams JS, Williams RS, Dovey CL, Guenther G, Tainer JA, Russell P EMBO J. 2010 Mar 17;29(6):1136-48. Epub 2010 Jan 21. PMID:20094029^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Williams JS, Williams RS, Dovey CL, Guenther G, Tainer JA, Russell P. gammaH2A binds Brc1 to maintain genome integrity during S-phase. EMBO J. 2010 Mar 17;29(6):1136-48. Epub 2010 Jan 21. PMID:20094029 doi:10.1038/emboj.2009.413

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3l41"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of S. pombe Brc1 BRCT5-BRCT6 domains in complex with phosphorylated H2A==
-<StructureSection load='3l41' size='340' side='right' caption='[[3l41]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
+<StructureSection load='3l41' size='340' side='right'caption='[[3l41]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3l41]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L41 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L41 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3l41]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L41 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L41 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1jnx|1jnx]], [[3hue|3hue]], [[2r1z|2r1z]], [[1t2v|1t2v]], [[2azm|2azm]], [[3l40|3l40]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">brc1, SPBC582.05c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l41 OCA], [https://pdbe.org/3l41 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l41 RCSB], [https://www.ebi.ac.uk/pdbsum/3l41 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l41 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l41 OCA], [http://pdbe.org/3l41 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l41 RCSB], [http://www.ebi.ac.uk/pdbsum/3l41 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l41 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BRC1_SCHPO BRC1_SCHPO]] Required for mitotic fidelity, specifically in the G2 phase of the cell cycle. Plays a role in chromatin organization.
+[https://www.uniprot.org/uniprot/BRC1_SCHPO BRC1_SCHPO] Required for mitotic fidelity, specifically in the G2 phase of the cell cycle. Plays a role in chromatin organization.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 16: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l4/3l41_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 34: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Cbs 356]]
+[[Category: Large Structures]]
-[[Category: Guenther, G]]
+[[Category: Schizosaccharomyces pombe]]
-[[Category: Tainer, J A]]
+[[Category: Guenther G]]
-[[Category: Williams, J S]]
+[[Category: Tainer JA]]
-[[Category: Williams, R S]]
+[[Category: Williams JS]]
-[[Category: Brc1]]
+[[Category: Williams RS]]
-[[Category: Brct domain]]
-[[Category: Cell cycle]]
-[[Category: Cell division]]
-[[Category: Dna repair]]
-[[Category: Mitosis]]
-[[Category: Phosphoserine binding domain]]
-[[Category: Tandem brct repeat]]

3l41

From Proteopedia

Current revision

Crystal Structure of S. pombe Brc1 BRCT5-BRCT6 domains in complex with phosphorylated H2A

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox