Endonuclease

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== Function ==
== Function ==
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'''Endonuclease''' (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. '''ENN-I''' operates on DNA with separate restriction site and cleavage pattern, while '''ENN-II''' operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN<ref>PMID:12483517</ref>.<br />
+
'''Endonuclease''' (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern.
 +
*'''ENN-I''' operates on DNA with separate restriction site and cleavage pattern.
 +
*'''ENN-II''' operates on overlapping restriction site and cleavage pattern.
 +
*'''ENN-III''' is a bifunctional DNA glycosylase that removes oxidized pyrimidines from DNA<ref>PMID:30959406</ref>.
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*'''ENN-IV''' cleaves the DNA backbone 5' of AP sites<ref>PMID:10458614</ref>.
 +
*'''ENN-V''' removes deaminated bases from DNA<ref>PMID:23263163</ref>.
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*'''ENN-VII''' recognizes mixmatches in DNA recombinational heteroduplexes and performs their incision<ref>PMID:21237725</ref>.
 +
*'''ENN-VIII''' removes radiolysis thymine products from phage DNA and cleaves the DNA at AP sites<ref>PMID:9405426</ref>.
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*'''Structure-specific ENN''' specific for DNA secondary structure rather than for its sequence<ref>PMID:28327556</ref>.
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*'''Intron-encoded ENN''' or '''homing ENN''' are encoded by genes with mobile, self-splicing introns. They promote the movement of DNA sequences from one chromosome location to another<ref>PMID:9665136</ref>. <br />
The '''Cas ENN''' proteins are part of '''CRISPR/Cas''' prokaryotic immune system which confers protection from foreign genetic elements like viruses. The '''CRISPR''' ('''C'''lustered '''R'''egularly '''I'''nterspersed '''S'''hort '''P'''alindromic '''R'''epeats) are DNA loci which are found in ca. 40% of the bacteria. The CRISPR/Cas system is being used lately as gene editing tool<ref>PMID:20056882</ref>. For more details see<br />
The '''Cas ENN''' proteins are part of '''CRISPR/Cas''' prokaryotic immune system which confers protection from foreign genetic elements like viruses. The '''CRISPR''' ('''C'''lustered '''R'''egularly '''I'''nterspersed '''S'''hort '''P'''alindromic '''R'''epeats) are DNA loci which are found in ca. 40% of the bacteria. The CRISPR/Cas system is being used lately as gene editing tool<ref>PMID:20056882</ref>. For more details see<br />
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* [[CRISPR-Cas]]<br />
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*[[CRISPR-Cas]]
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* [[CRISPR-Cas Part II]]<br />
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*CRISPR type I-A (Cascade) - see [[CRISPR subtype I-A]]
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* [[Cas9]]<br />
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*CRISPR type I-B (Cascade) - see [[CRISPR subtype I-B]]
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* [[CRISPR-Cas9]]<br />
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*CRISPR type I-C (Cascade) - see [[CRISPR subtype I-C]]
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* [[CRISPR type I-E (Cascade)]]<br />
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*CRISPR type I-E (Cascade) - see [[CRISPR type I-E (Cascade)|CRISPR subtype I-E]]
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* [[CRISPR type V]]<br />
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*(CRISPR type I-F (Csy1, Csy2, Csy3) - see [[CRISPR subtype I-F]]
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* [[CRISPR subtype I-A]]<br />
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*CRISPR type III-A (Csm complex) - see [[CRISPR subtype III-A (Csm complex)]]
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* [[CRISPR subtype I-B]]<br />
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*CRISPR type II-A - see [[CRISPR-Cas9]]
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* [[CRISPR subtype I-C]]<br />
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*CRISPR type V (Cpf1, C2c1, C2c3) - see [[CRISPR type V]]
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* [[CRISPR subtype I-F]]<br />
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*CRISPR type VI (Cas13a (previously known as C2c2), Cas13b, Cas13c, Cas13d) - see [[CRISPR type VI]]
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* [[CRISPR subtype III-A (Csm complex)]]<br />
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* [[CRISPR subtype III-B (Cmr complex)]]<br />
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* [[CRISPR subtype V (Cpf1)]]<br />
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* [[CRISPR type VI]]<br />
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* [[CRISPR type V (Cpf1)]]<br />
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'''Intron-encoded ENN''' or '''homing ENN''' are encoded by genes with mobile, self-splicing introns. They promote the movement of DNA sequences from one chromosome location to another<ref>PMID:9665136</ref>. <br />
+
See also<br />
See also<br />
* [[Apurinic-Apyrimidinic Endonuclease-1]]<br />
* [[Apurinic-Apyrimidinic Endonuclease-1]]<br />
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<scene name='49/496318/Cv/2'>DNA-protein binding</scene> (PDB code [[1rva]]).
<scene name='49/496318/Cv/2'>DNA-protein binding</scene> (PDB code [[1rva]]).
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</StructureSection>
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==3D structures of endonuclease==
==3D structures of endonuclease==
 +
[[Endonuclease 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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-
 
+
-
*ENN-I
+
-
 
+
-
**[[1bix]] - hENN – human<br />
+
-
**[[5um9]], [[5kse]], [[5k97]] – hENN (mutant) + DNA <br />
+
-
**[[1cfr]] – ENN – ''Citrobacter freundii''<br />
+
-
**[[1af5]] - CrENN – ''Chlamydomonas reinhardtii''<br />
+
-
**[[1fzr]], [[1m0d]], [[1m0i]], [[3cae]] – T7ENN - Enterobacteria phage T7<br />
+
-
**[[2g7e]], [[2g7f]] – VcENN – ''Vibrio cholera''<br />
+
-
**[[2vnd]] - VcENN (mutant) <br />
+
-
**[[2pu3]] - VsENN – ''Vibrio salmonicida''
+
-
 
+
-
*ENN-II
+
-
 
+
-
**[[1pvi]], [[1pvu]] - ENN – ''Proteus vulgaris''<br />
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-
**[[2wsh]] - T4ENN (mutant) - Enterobacteria phage T4<br />
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-
**[[3w52]], [[4cwm]], [[4cxo]], [[4cxp]], [[4cxv]] – ENN – ''Arabidopsis thaliana''<br />
+
-
 
+
-
*ENN-III
+
-
 
+
-
**[[2abk]], [[1ako]] - EcENN – ''Escherichia coli''<br />
+
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**[[1orn]], [[1orp]], [[1p59]] - GsENN + DNA – ''Geobacillus stearothermophilus''<br />
+
-
**[[4unf]] – DrENN-1 – ''Deinococcus radiodurans'' <br />
+
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**[[4uob]] – DrENN-3 <br />
+
-
 
+
-
*ENN-IV
+
-
 
+
-
**[[1qtw]] - EcENN <br />
+
-
**[[2nqh]] - EcENN (mutant) <br />
+
-
**[[1qum]] – EcENN + DNA<br />
+
-
**[[2nqj]], [[2nq9]], [[4k1g]] - EcENN (mutant) + DNA<br />
+
-
**[[1xp3]] - ENN – ''Bacillus anthracis''<br />
+
-
**[[3aal]] - GkENN – ''Geobacillus kaustophilus''<br />
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**[[3aam]] - TtENN – ''Thermus thermophilus''
+
-
 
+
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*ENN-V
+
-
 
+
-
**[[1eni]], [[1enj]], [[1enk]], [[2end]] - T4ENN <br />
+
-
**[[1vas]] - T4ENN + DNA<br />
+
-
**[[2w35]], [[2w36]], [[4b20]] - TmENN + DNA – ''Thermotoga maritima''<br />
+
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**[[3hd0]] - TmENN <br />
+
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**[[3ga2]] - BsENN (mutant) – ''Bacillus subtilis''<br />
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**[[3goc]] - ENN – ''Streptomyces avermitilis''<br />
+
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**[[2fcc]] – T4ENN + DNA<br />
+
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**[[4nsp]] – hENN catalytic domain<br />
+
-
 
+
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*ENN-VII
+
-
 
+
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**[[1en7]], [[1e7d]] - T4ENN <br />
+
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**[[1e7l]] - T4ENN (mutant) <br />
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**[[2qnc]], [[2qnf]] - T4ENN (mutant) + DNA
+
-
 
+
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*ENN-VIII
+
-
 
+
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**[[1q39]], [[1q39]] - EcENN <br />
+
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**[[1q3b]], [[1q3c]] - EcENN (mutant) <br />
+
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**[[1tdh]] – hENN <br />
+
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**[[1bix]] – hENN + DNA<br />
+
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**[[1k3x]], [[2ea0]], [[1k3w]], [[1k3x]], [[2ea0]], [[2opf]], [[2oq4]] - EcENN + DNA <br />
+
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**[[2opf]], [[2oq4]] - EcENN (mutant) + DNA
+
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+
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*AP ENN
+
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+
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**[[3ngf]] - ENN - ''Brucella melitensis''<br />
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**[[2j63]] – ENN – ''Leishmania major''<br />
+
-
 
+
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*Cas ENN
+
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+
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**[[1wj9]], [[4ej3]] – TtCas<br />
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**[[4n06]] – AfCas1 - ''Archaeoglobus fulgidus'' <br />
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**[[3god]] – PaCas1 – ''Pseudomonas aeruginosa''<br />
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**[[3nkd]] – EcCas1<br />
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**[[4wj0]] – PhCas1 - ''Pyrococcus horikoshii''<br />
+
-
**[[4xtk]] – TmCas1<br />
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**[[5fcl]] – Cas1 - ''Pectobacterium atrosepticum''<br />
+
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**[[4zkj]] - SpCas1 – ''Streptococcus pyogenes''<br />
+
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**[[3oq2]] – Cas2 – ''Desulfovibrio vulgaris''<br />
+
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**[[4mak]] – EcCas2<br />
+
-
**[[4p6i]], [[4qdl]] – EcCas1 + EcCas2<br />
+
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**[[3s4l]] – MjCas3 – ''Methanocaldococcus jannaschii''<br />
+
-
**[[4qqw]] – TfCas3 – ''Thermobifida fusca''<br />
+
-
**[[4qqx]] – TfCas3 + ATP <br />
+
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**[[4qqy]] – TfCas3 + ADP <br />
+
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**[[4qqz]] – TfCas3 + AMPPNP <br />
+
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**[[4q2c]] - TteCas3 – ''Thermobaclum terrenum''<br />
+
-
**[[4q2d]] - TteCas3 + ATP<br />
+
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**[[3i4h]] – Cas4 - ''Pyrobaculum calidifontis''<br /
+
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**[[4qr0]], [[4qr1]] - SpCas9 (Csn1) – ''Streptococcus pyogenes''<br />
+
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**[[4ilr]], [[3zfv]] – SsCas6 <br />
+
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**[[3i4h]] – PfCas6 - ''Pyrococcus furiosus''<br />
+
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**[[4dzd]] – EcCas6e<br />
+
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**[[4qr0]], [[4qr1]] - SpCas9 (Csn1) – ''Streptococcus pyogenes''<br />
+
-
**[[4qr0]], [[4qr1]], [[4cmp]], [[4cmq]] - SpCas9 <br />
+
-
**[[4ogc]], [[4oge]] – Cas9 II-C – ''Actinomyces naeslundii''<br />
+
-
**[[3ung]], [[3ur3]], [[4doz]], [[4w8y]] – PfCas10 CMR2 subunit <br />
+
-
**[[4h4k]] – PfCas10 CMR2+CMR3 subunits <br />
+
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**[[3w2v]] – PfCas10 CMR2+CMR3 subunits + AMP<br />
+
-
**[[3w2w]] – PfCas10 CMR2+CMR3 subunits + ATP<br />
+
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**[[4gkf]] – PfCas10 CMR5 subunit<br />
+
-
**[[4an8]], [[4f3e]] – TtCasA (Cse1)<br />
+
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**[[4h3t]] - CasA (Cse1) – ''Acidimicrobium ferrooxidans''<br />
+
-
**[[4h79]] - TfCasB (Cse2)<br />
+
-
**[[3wa8]] - CasB (Cse2) – ''Meiothermus ruber''<br />
+
-
**[[4h7a]] - TtCasB (Cse2)<br />
+
-
**[[4tvx]] – EcCasA (Cse1) + CasE<br />
+
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**[[3qhq]] - Csn2 – ''Streptococcus agalactiae''<br />
+
-
**[[3toc]], [[3v7f]] – SpCsn2<br />
+
-
**[[3ps0]] – SsCsa2<br />
+
-
 
+
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*Cas ENN complex with RNA and DNA
+
-
 
+
-
**[[5dlj]], [[5dqt]], [[5dqu]], [[5dqz]], [[5ds4]], [[5ds5]], [[5ds6]], [[5vvl]], [[5vvk]], [[5vvj]] – EcCas1 + EcCas2 + DNA<br />
+
-
**[[5wfe]] – EcCas1 + EcCas2 + IHF-a + IHF-b + DNA<br />
+
-
**[[5xvp]], [[5xvo]], [[5xvn]] – Cas1 + Cas2 + DNA – ''Enterococcus faecalis''<br />
+
-
**[[4ill]], [[4ilm]] – SsCas6 + RNA - ''Sulfolobus solfataricus''<br />
+
-
**[[5uz9]] – PaCsa5f + Cas7f + Cas8f + anti-crispr protein + RNA<br />
+
-
**[[6b48]], [[6b47]], [[6b46]], [[6b45]] – PaCsa6 + Csy1 + Csy2 + Csy3 + anti-crispr protein + RNA<br />
+
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**[[6b44]] – PaCsa6 + Csy1 + Csy2 + Csy3 + anti-crispr protein + DNA<br />
+
-
**[[3pkm]] – PfCas6 + RNA<br />
+
-
**[[2y8w]], [[2y8y]], [[3qrp]], [[3qrq]], [[2qrr]] – TtCas6e (Cse3) + RNA<br />
+
-
**[[2xli]], [[2xlj]], [[2xlk]], [[4al7]] – PaCas6f (Csy4) + RNA <br />
+
-
**[[4al5]], [[4al6]] - PaCas6f (Csy4) (mutant) + RNA <br />
+
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**[[4zt0]] - SpCas9 + RNA <br />
+
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**[[4zt9]] - SpCas9 (mutant) + RNA <br />
+
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**[[5f9r]] - SpCas9 + RNA + DNA<br />
+
-
**[[4oo8]], [[4un3]], [[4un4]], [[4un5]], [[5fw1]], [[5fw2]], [[5fw3]], [[5fq5]], [[5b2r]], [[5b2s]], [[5b2t]], [[5y36]] - SpCas9 (Csn1) (mutant) + RNA + DNA<br />
+
-
**[[5x2h]], [[5x2g]] – Cas9 + RNA + DNA – ''Campylobacter jejuni''<br />
+
-
**[[5czz]] - SaCas9 (Csn1) + RNA + DNA - ''Staphylococcus aureus''<br />
+
-
**[[5axw]] - SaCas9 (Csn1) (mutant) + RNA + DNA <br />
+
-
**[[5b2o]], [[5b2p]], [[5b2q]] - FtCas9 (mutant) + RNA + DNA – ''Francisella tularensis''<br />
+
-
**[[4u7u]] – EcCasA (Cse1) + CasB + CasC + CasD + CasE + RNA<br />
+
-
**[[4qyz]], [[5h9f]], [[5h9e]] – EcCasA (Cse1) + CasB + CasC + CasD + CasE + RNA + DNA<br />
+
-
**[[5wqe]] – AaCas C2C1 + RNA – ''Alicyclobacillus acidoterrestris''<br />
+
-
**[[5u34]] – AaCas C2C1 (mutant) + RNA <br />
+
-
**[[5u30]], [[5u31]], [[5u33]] – AaCas C2C1 + RNA + DNA<br />
+
-
**[[5kk5]], [[5b43]] – AcCas Cpf1 + RNA + DNA – ''Acidaminococcus''<br />
+
-
**[[5xh7]], [[5xh6]] – AcCas Cpf1 (mutant) + RNA + DNA <br />
+
-
**[[5ng6]] – FtCas Cpf1 + RNA <br />
+
-
**[[5nfv]], [[5mga]] – FtCas Cpf1 + RNA + DNA <br />
+
-
 
+
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*EcoKI ENN
+
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+
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**[[2ar0]] – EcENN (mutant)<br />
+
-
 
+
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*Ecoo109I ENN
+
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+
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**[[1wtd]] - EcENN<br />
+
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**[[1wte]] – EcENN + DNA<br />
+
-
 
+
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*EcoRI ENN
+
-
 
+
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**[[1qc9]] - EcENN<br />
+
-
**[[1eri]], [[1ckq]], [[1cl8]], [[1qps]] – EcENN + DNA<br />
+
-
**[[1qrh]], [[1qri]], [[2oxv]] - EcENN (mutant) + DNA
+
-
 
+
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*EcoRII ENN
+
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+
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**[[1na6]] – EcENN<br />
+
-
**[[3hqf]], [[3hqg]] - EnENN + DNA
+
-
 
+
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*EcoRV ENN
+
-
 
+
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**[[1rve]], [[1az3]] – EcENN<br />
+
-
**[[1az4]] - EcENN (mutant) <br />
+
-
**[[2rve]], [[4rve]], [[1rva]], [[1rvb]], [[1rvc]], [[1az0]], [[1bsu]], [[1bua]], [[1bgb]], [[1rv5]], [[1b94]], [[1b95]], [[1eo3]], [[1eo4]], [[1eon]], [[1eoo]], [[1eop]], [[2b0d]], [[2b0e]], [[5hlk]] - EcENN + DNA<br />
+
-
**[[1bss]], [[1b96]], [[1b97]], [[1stx]], [[1suz]], [[1sx5]], [[1sx8]], [[2ge5]] - EcENN (mutant) + DNA<br />
+
-
**[[5f8a]] - EcENN + DNA + Lu<br />
+
-
 
+
-
*BamHI ENN
+
-
 
+
-
**[[1bam]] – BaENN – ''Bacillus amyloliquefaciens''<br />
+
-
**[[1bhm]], [[3bam]], [[2bam]], [[1esg]] - BaENN + DNA
+
-
 
+
-
*FokI ENN
+
-
 
+
-
**[[1fok]] – PoENN + DNA – ''Planomicrobium okeanokoites''<br />
+
-
**[[2fok]] – PoENN
+
-
 
+
-
*LAGLIDAD ENN
+
-
 
+
-
**[[4z1x]] – fyENN + DNA – fission yeast<br />
+
-
**[[5e5o]] – ENN + DNA – ''Sordaria macropora''<br />
+
-
 
+
-
*PvuII ENN
+
-
 
+
-
**[[3pvi]] - PvENN (mutant) + DNA – ''Proteus vulgaris''<br />
+
-
**[[1k0z]], [[1h56]], [[3ksk]] - PvENN<br />
+
-
**[[1ni0]] - PvENN (mutant) <br />
+
-
**[[2pvi]], [[1eyu]], [[1f0o]] - PvENN + DNA
+
-
 
+
-
*tRNA-splicing ENN
+
-
 
+
-
**[[1a79]], [[1a76]], [[1a77]] - MjENN – ''Methanocaldococcus jannaschii''<br />
+
-
**[[2cv8]] - ENN – ''Sulfolobus tokodaii''<br />
+
-
**[[2gw6]] – hENN subunit sen15 – NMR<br />
+
-
**[[2gjw]] - AfENN + RNA –'' Archaeoglobus fulgidus''<br />
+
-
**[[1r0v]], [[1r11]] – AfENN
+
-
**[[2ohc]], [[2ohe]] - TaENN – ''Thermoplasma acidophilum''<br />
+
-
**[[2zyz]] – ENN – ''Pyrobaculum aerophilum''<br />
+
-
**[[3iey]] - ENN + NEQ261 – ''Nanoarchaeum equitans''<br />
+
-
**[[3ajv]] - ApENN (mutant) – ''Aeropyrum pernix''<br />
+
-
**[[3p1y]] - ApENN /AfENN<br />
+
-
**[[3p1z]] - ApENN + protein
+
-
 
+
-
*VSR ENN
+
-
 
+
-
**[[1vsr]] – EcENN<br />
+
-
**[[1cw0]] - EcENN + DNA
+
-
 
+
-
*BglI ENN
+
-
 
+
-
**[[1dmu]], [[1d2i]] – BsENN + DNA <br />
+
-
**[[1dfm]] - BsENN (mutant) + DNA
+
-
 
+
-
*BglII ENN
+
-
 
+
-
**[[1es8]] - BsENN
+
-
 
+
-
*MunI ENN
+
-
 
+
-
**[[1d02]] - ENN (mutant) + DNA - ''Mycoplasma''
+
-
 
+
-
*Sm ENN
+
-
 
+
-
**[[1smn]], [[1qae]], [[1ql0]], [[1g8t]] – ENN – ''Serratia marcescens''
+
-
 
+
-
*NaeI ENN
+
-
 
+
-
**[[1ev7]] - LaENN – ''Lechevalieria aerocolonigenes''<br />
+
-
**[[1iaw]] - LaENN + DNA
+
-
 
+
-
*NgoMIV ENN
+
-
 
+
-
**[[1fiu]], [[4abt]] - ENN + DNA – ''Neisseria gonorrheae''
+
-
 
+
-
*NEI ENN
+
-
 
+
-
**[[1tdh]] - hENN
+
-
 
+
-
*BsoBI ENN
+
-
 
+
-
**[[1dc1]] - GsENN + DNA
+
-
 
+
-
*Bse634I ENN
+
-
 
+
-
**[[1knv]] – GsENN<br />
+
-
**[[3v1z]] – GsENN + DNA<br />
+
-
**[[3v20]], [[3v21]] - GsENN (mutant) + DNA
+
-
 
+
-
*HincII ENN
+
-
 
+
-
**[[1kc6]], [[1tw8]], [[1xhu]], [[1xhv]], [[1tx3]], [[2gie]] - HiENN + DNA – ''Haemophilus influenza''<br />
+
-
**[[2gig]], [[2gih]], [[2gii]], [[2gij]], [[3e3y]], [[3e40]], [[3e41]], [[3e42]], [[3e43]], [[3e44]], [[3e45]], [[3ebc]] - HiENN (mutant) + DNA<br />
+
-
**[[2aud]] – HiENN
+
-
 
+
-
*HinDIII ENN
+
-
 
+
-
**[[2e52]], [[3a4k]] - HiENN + DNA
+
-
 
+
-
*HinP1I ENN
+
-
 
+
-
**[[1ynm]] – HiENN<br />
+
-
**[[2fkc]], [[2fkh]], [[2fl3]], [[2flc]] - HiENN + DNA
+
-
 
+
-
*Flap ENN
+
-
 
+
-
**[[1mc8]] - PhENN (mutant) <br />
+
-
**[[3ory]]– ENN – ''Desulfurococcus amylolyticus''<br />
+
-
**[[3q8k]], [[3q8l]], [[3q8m]] - hENN + DNA<br />
+
-
**[[5fv7]] - hENN + inhibitor<br />
+
-
**[[1rxv]], [[1rxw]] - AfENN + DNA<br />
+
-
**[[1ul1]] - hENN + PCNA <br />
+
-
**[[2izo]] - SsENN + PCNA – ''Sulfolobus solfataricus''<br />
+
-
**[[1a76]], [[1a77]] - MjENN <br />
+
-
**[[1b43]] – PfENN – ''Pyrococcus furiosus''<br />
+
-
**[[1rxz]] - AfENN + PCNA <br />
+
-
**[[4wa8]] – ENN – ''Methanopyrus kandleri''<br />
+
-
 
+
-
*DNA mismatch ENN
+
-
 
+
-
**[[1odg]] - EcENN + DNA<br />
+
-
**[[1h7u]] – hENN<br />
+
-
**[[5u5r]] – hENN + importin<br />
+
-
 
+
-
*Vvn ENN
+
-
 
+
-
**[[1ouo]] – VvENN – ''Vibrio vulnificus''<br />
+
-
**[[1oup]], [[2ivk]] - VvENN + DNA
+
-
 
+
-
*Structure-specific ENN
+
-
 
+
-
**[[4xm5]] – CgENN SLX1 subunit – ''Candida glabrata''<br />
+
-
**[[4xlg]] – CgENN SLX1 (mutant)+SLX4 subunits<br />
+
-
**[[4zdt]] – fyENN SLX1+SLX4 subunits<br />
+
-
**[[4zou]] – hENN SLX4 subunit BTB domain<br />
+
-
**[[4uyi]] – hENN SLX4 subunit BTB domain (mutant)<br />
+
-
 
+
-
*BfiI ENN
+
-
 
+
-
**[[2c1l]] – ENN – ''Bacillus firmus''
+
-
 
+
-
*SfiI ENN
+
-
 
+
-
**[[2ezv]], [[2f03]] – ENN + DNA – synthetic
+
-
 
+
-
*Ecl18kI ENN
+
-
 
+
-
**[[2fqz]], [[2gb7]] – ENN + DNA – ''Enterobacter cloacae''
+
-
 
+
-
*SdaI ENN
+
-
 
+
-
**[[2ixs]] – ENN – ''Streptomyces diastaticus''
+
-
 
+
-
*PabI ENN
+
-
 
+
-
**[[2dvy]] – ENN – ''Pyrococcus abyssi''
+
-
 
+
-
*PspGI ENN
+
-
 
+
-
**[[3bm3]] – ENN – ''Pyrococcus''
+
-
 
+
-
*HNH ENN
+
-
 
+
-
**[[4h6d]] – ENN – ''Geobacter metallireducens''<br />
+
-
**[[5h0o]], [[5h0m]] – ENN – Geobacillus virus E2<br />
+
-
**[[5cxr]] – ENN + pyrazole derivative – Influenza virus <br />
+
-
 
+
-
*UV damage ENN
+
-
 
+
-
**[[2j6v]], [[3bzg]], [[3c0s]] – TtENN <br />
+
-
**[[3bzj]], [[3c0l]], [[3c0q]] - TtENN (mutant)<br />
+
-
**[[3tc3]] - SaENN <br />
+
-
**[[4gle]] - SaENN + DNA<br />
+
-
 
+
-
*NotI ENN
+
-
 
+
-
**[[3bvq]] - NoENN – ''Nocardia otitidiscaviarum''<br />
+
-
**[[3c25]] - NoENN + DNA
+
-
 
+
-
*BpujI ENN
+
-
 
+
-
**[[2vla]], [[3s1s]] – BpENN – ''Bacillus pumilus''<br />
+
-
**[[5hnf]], [[5hnh]], [[5hlt]] – BpENN + DNA <br />
+
-
 
+
-
*Sau3AI ENN
+
-
 
+
-
**[[2reu]] – SaENN C terminal – ''Staphylococcus aureus''<br />
+
-
**[[4pxg]] – SaENN (mutant) <br />
+
-
 
+
-
*HpyI ENN
+
-
 
+
-
**[[3fc3]], [[3gox]] – HpENN99 + DNA – ''Helicobacter pylori''<br />
+
-
**[[3or3]] – HpENN88 + DNA
+
-
 
+
-
*PacI ENN
+
-
 
+
-
**[[3ldy]], [[3m7k]] - PaENN + DNA – ''Psdeudomonas alcaligenes''
+
-
 
+
-
*Crossover junction ENN
+
-
 
+
-
**[[2kpf]] - mENN – mouse<br />
+
-
**[[2ziw]], [[2zix]], [[2ziu]] – hENN EME1 + Mus81<br />
+
-
**[[2ziv]] – hENN EME1 (mutant) + Mus81<br />
+
-
**[[4p0p]], [[4p0q]], [[4p0r]], [[4p0s]] – hENN EME1 + Mus81 + DNA<br />
+
-
 
+
-
*OkrAI ENN
+
-
 
+
-
**[[3odh]] - ENN + DNA – ''Oceanobacter kriegii''
+
-
 
+
-
*BspD6I ENN
+
-
 
+
-
**[[2ewf]] - ENN – ''Bacillus''
+
-
 
+
-
*ThaI ENN
+
-
 
+
-
**[[3ndh]] - TaENN + DNA
+
-
 
+
-
*Xpf ENN
+
-
 
+
-
**[[1z00]], [[2a1j]] - hENN C terminal + DNA excision repair protein<br />
+
-
**[[2aq0]] – hENN residues 823-905 – NMR<br />
+
-
**[[2kn7]] – hENN residues 823-905 + DNA – NMR<br />
+
-
**[[2bgw]] - ApENN + DNA <br />
+
-
**[[2bhn]] - ApENN <br />
+
-
 
+
-
*Mazf3 ENN
+
-
 
+
-
**[[5uct]], [[5cca]] - MtENN – ''Mycobacterium tuberculosis''<br />
+
-
 
+
-
*Vapc ENN
+
-
 
+
-
**[[5wzf]], [[5wz4]] – MtENN-20 <br />
+
-
**[[5ed0]], [[5ecy]] – SfENN – ''Shigella flexneri''<br />
+
-
**[[5ecy]], [[5ecw]], [[5ecd]] – SfENN (mutant)<br />
+
-
 
+
-
*Muts2 ENN
+
-
 
+
-
**[[4och]], [[4od6]] - DrENN SMR domain<br />
+
-
 
+
-
*Endoms ENN
+
-
 
+
-
**[[5gkj]] – TkENN (mutant) – ''Thermococcus kodakarensis''<br />
+
-
**[[5gke]], [[5gkf]], [[5gkg]], [[5gkh]], [[5gki]] – TkENN (mutant) + DNA<br />
+
-
 
+
-
*ENN G
+
-
 
+
-
**[[5gkc]], [[4qn0]] – CeENN (mutant) – ''Caenorhabdfitis elegans''<br />
+
-
**[[5gkp]] – CeENN (mutant) + DNA <br />
+
-
 
+
-
*ENN S
+
-
 
+
-
**[[5ybb]] – ENN + R-M system + DNA – ''Caldanaerobacter subterraneus'' <br />
+
-
 
+
-
*ENN V
+
-
 
+
-
**[[5aoy]] – mENN (mutant) <br />
+
-
**[[4xpu]] – EcENN <br />
+
-
 
+
-
*Apurinic-apyrimidinic ENN
+
-
 
+
-
**[[5cfg]] – hENN <br />
+
-
**[[5dff]], [[5dfh]], [[5dfi]], [[5dfj]], [[5dg0]], [[5wn5]], [[5wn4]], [[5wn3]], [[5wn2]], [[5wn1]], [[5wn0]] – hENN + DNA<br />
+
-
 
+
-
*'''Intron-encoded endonuclease'''
+
-
 
+
-
*I-PpoI ENN
+
-
 
+
-
**[[1a74]], [[1ipp]], [[1a73]], [[1cz0]] - PpENN + DNA – ''Physarum polycephalum''<br />
+
-
**[[1cyq]], [[1evw]], [[2o6m]] - PpENN (mutant) + DNA<br />
+
-
**[[1evx]] - PpENN
+
-
 
+
-
*I-CreI ENN
+
-
 
+
-
**[[1bp7]], [[1g9y]], [[1g9z]], [[1n3e]], [[1n3f]], [[2vbj]], [[2vbl]], [[2vbn]], [[2vbo]], [[4aqu]], [[4aqx]] - CrENN + DNA <br />
+
-
**[[1u0c]], [[1u0d]], [[1t9i]], [[1t9j]], [[2i3p]], [[2i3q]], [[4aab]], [[4aad]], [[4aae]], [[4aaf]], [[4aag]] - CrENN (mutant) + DNA<br />
+
-
**[[2o7m]] - CrENN
+
-
 
+
-
*I-CeuI ENN
+
-
 
+
-
**[[2ex5]] - ENN (mutant) + DNA – ''Chlamydomonas eugametos''
+
-
 
+
-
*I-DmoI ENN
+
-
 
+
-
**[[1b24]], [[2vs7]], [[2vs8]], [[4un7]], [[4un8]], [[4un9]], [[4una]], [[4unb]], [[4unc]], [[4ut0]], [[4d6n]], [[4d6o]], [[5ak9]], [[5a0w]], [[5o6g]], [[5o6i]] - DmENN + DNA – ''Desulfurococcus mobilis''<br />
+
-
**[[5akf]], [[5akm]], [[5akn]] - DmENN (mutant) + DNA <br />
+
-
**[[1mow]] – DmENN/CrENN + DNA
+
-
 
+
-
*I-AniI ENN
+
-
 
+
-
**[[1p8k]], [[2qoj]], [[3uvf]] - EnENN + DNA – ''Emericella nidulans''<br />
+
-
**[[3eh8]] - EnENN (mutant) + DNA
+
-
 
+
-
*I-HmuI ENN
+
-
 
+
-
**[[1u3e]] - ENN + DNA – Bacillus phage spo1
+
-
 
+
-
*I-TevI ENN
+
-
 
+
-
**[[1i3j]], [[1t2t]] - T4ENN + DNA<br />
+
-
**[[1ln0]], [[1mk0]] – T4ENN (mutant)
+
-
 
+
-
*I-SceI ENN
+
-
 
+
-
**[[1dfa]], [[1ef0]] – yENN – yeast<br />
+
-
**[[1gpp]] – yENN (mutant) <br />
+
-
**[[1lws]], [[1lwt]], [[1r7m]], [[3c0w]], [[3c0x]], [[3ool]], [[5a0m]] - yENN + DNA<br />
+
-
**[[3oor]] - yENN (mutant) + DNA
+
-
 
+
-
*I-PkoII ENN
+
-
 
+
-
**[[2cw7]], [[2cw8]] – TkENN
+
-
 
+
-
*I-MspI ENN
+
-
 
+
-
**[[1sa3]], [[1yfi]], [[2fld]] – ENN + DNA – ''Moraxella''
+
-
 
+
-
*I-PfuI ENN
+
-
 
+
-
**[[1dq3]] – PfENN
+
-
 
+
-
*I-Tsp061I ENN
+
-
 
+
-
**[[2dch]] – ENN – ''Thermoproteus''
+
-
 
+
-
*I-Vdi141I ENN
+
-
 
+
-
**[[3e54]] – ENN – ''Vulcanisaeta distribute''
+
-
 
+
-
*I-MtuI ENN
+
-
 
+
-
**[[3ifj]], [[3igd]] - MtENN (mutant) <br />
+
-
**[[2l8l]] - MtENN
+
-
 
+
-
*I-MsoI ENN
+
-
 
+
-
**[[1m5x]], [[3fd2]] – MoENN + DNA – ''Monomastix''<br />
+
-
**[[3ko2]] – MoENN (mutant) + DNA
+
-
 
+
-
*I-GzeMII ENN
+
-
 
+
-
**[[4efj]] – ENN + DNA – ''Gibberella zeae''
+
-
 
+
-
*I-tRNA ENN
+
-
 
+
-
**[[4fz2]] – MoENN – ''Candidatus micrarchaeum''<br />
+
-
 
+
-
*I-Cvui ENN
+
-
 
+
-
**[[5a78]], [[5a72]], [[5a74]], [[5a77]], [[5a78]] – ENN + DNA – ''Chlorella vulgaris''<br />
+
-
}}
 
== References ==
== References ==
<references/>
<references/>
Line 561: Line 52:
[[Category:Topic Page]]
[[Category:Topic Page]]
 +
[[Category:Endonuclease]]

Current revision

E. coli EcoRV endonuclease dimer (magenta, green) complex with DNA, (PDB code 1rva)

Drag the structure with the mouse to rotate

References

  1. ↑ Sarre A, Stelter M, Rollo F, De Bonis S, Seck A, Hognon C, Ravanat JL, Monari A, Dehez F, Moe E, Timmins J. The three Endonuclease III variants of Deinococcus radiodurans possess distinct and complementary DNA repair activities. DNA Repair (Amst). 2019 Jun;78:45-59. PMID:30959406 doi:10.1016/j.dnarep.2019.03.014
  2. ↑ Hosfield DJ, Guan Y, Haas BJ, Cunningham RP, Tainer JA. Structure of the DNA repair enzyme endonuclease IV and its DNA complex: double-nucleotide flipping at abasic sites and three-metal-ion catalysis. Cell. 1999 Aug 6;98(3):397-408. PMID:10458614
  3. ↑ Cao W. Endonuclease V: an unusual enzyme for repair of DNA deamination. Cell Mol Life Sci. 2013 Sep;70(17):3145-56. PMID:23263163 doi:10.1007/s00018-012-1222-z
  4. ↑ Shcherbakov VP, Plugina L, Shcherbakova T. Endonuclease VII is a key component of the mismatch repair mechanism in bacteriophage T4. DNA Repair (Amst). 2011 Apr 3;10(4):356-62. PMID:21237725 doi:10.1016/j.dnarep.2010.12.006
  5. ↑ Jiang D, Hatahet Z, Melamede RJ, Kow YW, Wallace SS. Characterization of Escherichia coli endonuclease VIII. J Biol Chem. 1997 Dec 19;272(51):32230-9. PMID:9405426
  6. ↑ Dehé PM, Gaillard PHL. Control of structure-specific endonucleases to maintain genome stability. Nat Rev Mol Cell Biol. 2017 May;18(5):315-330. PMID:28327556 doi:10.1038/nrm.2016.177
  7. ↑ Flick KE, Jurica MS, Monnat RJ Jr, Stoddard BL. DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI. Nature. 1998 Jul 2;394(6688):96-101. PMID:9665136 doi:10.1038/27952
  8. ↑ Horvath P, Barrangou R. CRISPR/Cas, the immune system of bacteria and archaea. Science. 2010 Jan 8;327(5962):167-70. doi: 10.1126/science.1179555. PMID:20056882 doi:http://dx.doi.org/10.1126/science.1179555

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