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| - | [[Image:430d.gif|left|200px]] | |
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| - | {{Structure
| + | ==STRUCTURE OF SARCIN/RICIN LOOP FROM RAT 28S RRNA== |
| - | |PDB= 430d |SIZE=350|CAPTION= <scene name='initialview01'>430d</scene>, resolution 2.10Å
| + | <StructureSection load='430d' size='340' side='right'caption='[[430d]], [[Resolution|resolution]] 2.10Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=CBV:5-BROMOCYTIDINE+5'-(DIHYDROGEN+PHOSPHATE)'>CBV</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[430d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=430D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=430D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBV:5-BROMOCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>CBV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=430d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=430d OCA], [https://pdbe.org/430d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=430d RCSB], [https://www.ebi.ac.uk/pdbsum/430d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=430d ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=430d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=430d OCA], [http://www.ebi.ac.uk/pdbsum/430d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=430d RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''STRUCTURE OF SARCIN/RICIN LOOP FROM RAT 28S RRNA'''
| + | [[Category: Rattus norvegicus]] |
| - | | + | [[Category: Chan YL]] |
| - | | + | [[Category: Correll CC]] |
| - | ==Overview==
| + | [[Category: Munishkin A]] |
| - | The structure of a 29-nucleotide RNA containing the sarcin/ricin loop (SRL) of rat 28 S rRNA has been determined at 2.1 A resolution. Recognition of the SRL by elongation factors and by the ribotoxins, sarcin and ricin, requires a nearly universal dodecamer sequence that folds into a G-bulged cross-strand A stack and a GAGA tetraloop. The juxtaposition of these two motifs forms a distorted hairpin structure that allows direct recognition of bases in both grooves as well as recognition of nonhelical backbone geometry and two 5'-unstacked purines. Comparisons with other RNA crystal structures establish the cross-strand A stack and the GNRA tetraloop as defined and modular RNA structural elements. The conserved region at the top is connected to the base of the domain by a region presumed to be flexible because of the sparsity of stabilizing contacts. Although the conformation of the SRL RNA previously determined by NMR spectroscopy is similar to the structure determined by x-ray crystallography, significant differences are observed in the "flexible" region and to a lesser extent in the G-bulged cross-strand A stack.
| + | [[Category: Ren Z]] |
| - | | + | [[Category: Steitz TA]] |
| - | ==About this Structure==
| + | [[Category: Wool IG]] |
| - | 430D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=430D OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Crystal structure of the ribosomal RNA domain essential for binding elongation factors., Correll CC, Munishkin A, Chan YL, Ren Z, Wool IG, Steitz TA, Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13436-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9811818 9811818]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Chan, Y-L.]] | + | |
| - | [[Category: Correll, C C.]] | + | |
| - | [[Category: Munishkin, A.]] | + | |
| - | [[Category: Ren, Z.]] | + | |
| - | [[Category: Steitz, T A.]] | + | |
| - | [[Category: Wool, I G.]] | + | |
| - | [[Category: base triple]]
| + | |
| - | [[Category: blunt stem]]
| + | |
| - | [[Category: double helix]]
| + | |
| - | [[Category: hairpin]]
| + | |
| - | [[Category: ribonucleic acid]]
| + | |
| - | [[Category: tetraloop mismatched]]
| + | |
| - | [[Category: u-rna]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:37:06 2008''
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