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| | ==Crystal structure of HL4E10 Fab, a hamster Ab stimulatory for gammadelta T cells== | | ==Crystal structure of HL4E10 Fab, a hamster Ab stimulatory for gammadelta T cells== |
| - | <StructureSection load='3mj8' size='340' side='right' caption='[[3mj8]], [[Resolution|resolution]] 2.94Å' scene=''> | + | <StructureSection load='3mj8' size='340' side='right'caption='[[3mj8]], [[Resolution|resolution]] 2.94Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3mj8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cricetulus_migratorius Cricetulus migratorius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MJ8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3mj8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Nothocricetulus_migratorius Nothocricetulus migratorius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MJ8 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3mj6|3mj6]], [[3mj7|3mj7]], [[3mj9|3mj9]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.94Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mj8 OCA], [http://pdbe.org/3mj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mj8 RCSB], [http://www.ebi.ac.uk/pdbsum/3mj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mj8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mj8 OCA], [https://pdbe.org/3mj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mj8 RCSB], [https://www.ebi.ac.uk/pdbsum/3mj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mj8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | ==See Also== | | ==See Also== |
| | *[[Antibody 3D structures|Antibody 3D structures]] | | *[[Antibody 3D structures|Antibody 3D structures]] |
| | + | *[[Sandbox 20009|Sandbox 20009]] |
| | + | *[[3D structures of non-human antibody|3D structures of non-human antibody]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cricetulus migratorius]] | + | [[Category: Large Structures]] |
| - | [[Category: Verdino, P]] | + | [[Category: Nothocricetulus migratorius]] |
| - | [[Category: Wilson, I A]] | + | [[Category: Verdino P]] |
| - | [[Category: Antibody]] | + | [[Category: Wilson IA]] |
| - | [[Category: Hamster igg]]
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| - | [[Category: Immune system]]
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| Structural highlights
Publication Abstract from PubMed
Hamsters are widely used to generate monoclonal antibodies against mouse, rat, and human antigens, but sequence and structural information for hamster immunoglobulins is sparse. To our knowledge, only three hamster IgG sequences have been published, all of which use kappa light chains, and no three-dimensional structure of a hamster antibody has been reported. We generated antibody HL4E10 as a probe to identify novel costimulatory molecules on the surface of gammadelta T cells which lack the traditional alphabeta T cell co-receptors CD4, CD8, and the costimulatory molecule CD28. HL4E10 binding to gammadelta T cell, surface-expressed, Junctional Adhesion Molecule-Like (JAML) protein leads to potent costimulation via activation of MAP kinase pathways and cytokine production, resulting in cell proliferation. The cDNA sequence of HL4E10 is the first example of a hamster lambda light chain and only the second known complete hamster heavy chain sequence. The crystal structure of the HL4E10 Fab at 2.95 A resolution reveals a rigid combining site with pockets faceted by solvent-exposed tyrosine residues, which are structurally optimized for JAML binding. The characterization of HL4E10 thus comprises a valuable addition to the spartan database of hamster immunoglobulin genes and structures. As the HL4E10 antibody is uniquely costimulatory for gammadelta T cells, humanized versions thereof may be of clinical relevance in treating gammadelta T cell dysfunction-associated diseases, such as chronic non-healing wounds and cancer.
cDNA sequence and Fab crystal structure of HL4E10, a hamster IgG lambda light chain antibody stimulatory for gammadelta T cells.,Verdino P, Witherden DA, Podshivalova K, Rieder SE, Havran WL, Wilson IA PLoS One. 2011;6(5):e19828. doi: 10.1371/journal.pone.0019828. Epub 2011 May 24. PMID:21629689[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Verdino P, Witherden DA, Podshivalova K, Rieder SE, Havran WL, Wilson IA. cDNA sequence and Fab crystal structure of HL4E10, a hamster IgG lambda light chain antibody stimulatory for gammadelta T cells. PLoS One. 2011;6(5):e19828. doi: 10.1371/journal.pone.0019828. Epub 2011 May 24. PMID:21629689 doi:10.1371/journal.pone.0019828
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