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| | ==Crystal structure of the BTB domain from the Miz-1/ZBTB17 transcription regulator== | | ==Crystal structure of the BTB domain from the Miz-1/ZBTB17 transcription regulator== |
| - | <StructureSection load='3m52' size='340' side='right' caption='[[3m52]], [[Resolution|resolution]] 2.59Å' scene=''> | + | <StructureSection load='3m52' size='340' side='right'caption='[[3m52]], [[Resolution|resolution]] 2.59Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3m52]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M52 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3m52]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M52 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m5b|3m5b]], [[3m4t|3m4t]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MIZ1, ZBTB17, ZNF151, ZNF60 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m52 OCA], [https://pdbe.org/3m52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m52 RCSB], [https://www.ebi.ac.uk/pdbsum/3m52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m52 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m52 OCA], [http://pdbe.org/3m52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m52 RCSB], [http://www.ebi.ac.uk/pdbsum/3m52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m52 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ZBT17_HUMAN ZBT17_HUMAN]] Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment (By similarity). Transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation.<ref>PMID:9312026</ref> <ref>PMID:9308237</ref> <ref>PMID:19164764</ref> | + | [https://www.uniprot.org/uniprot/ZBT17_HUMAN ZBT17_HUMAN] Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment (By similarity). Transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation.<ref>PMID:9312026</ref> <ref>PMID:9308237</ref> <ref>PMID:19164764</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Chen, L]] | + | [[Category: Large Structures]] |
| - | [[Category: Cuesta-Seijo, J A]] | + | [[Category: Chen L]] |
| - | [[Category: Prive, G G]] | + | [[Category: Cuesta-Seijo JA]] |
| - | [[Category: Stogios, P J]]
| + | [[Category: Prive GG]] |
| - | [[Category: Alpha/beta protein]]
| + | [[Category: Stogios PJ]] |
| - | [[Category: Btb domain]]
| + | |
| - | [[Category: Btb/poz domain]]
| + | |
| - | [[Category: Developmental protein]]
| + | |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Metal-binding]] | + | |
| - | [[Category: Miz-1]] | + | |
| - | [[Category: Miz-1 protein]]
| + | |
| - | [[Category: Miz1]]
| + | |
| - | [[Category: Myc-interacting zinc finger protein]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Poz domain]]
| + | |
| - | [[Category: Protein-protein interaction domain]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription activator]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| - | [[Category: Transcription regulator]]
| + | |
| - | [[Category: Zfp-151]]
| + | |
| - | [[Category: Zfp151]]
| + | |
| - | [[Category: Zinc finger and btb domain-containing protein 17]]
| + | |
| - | [[Category: Zinc finger protein 151]]
| + | |
| - | [[Category: Zinc finger protein 60]]
| + | |
| - | [[Category: Zinc-finger]]
| + | |
| - | [[Category: Zinc-finger protein]]
| + | |
| - | [[Category: Znf151]]
| + | |
| Structural highlights
Function
ZBT17_HUMAN Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment (By similarity). Transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The BTB domain is a widely distributed protein-protein interaction motif that is often found at the N-terminus of zinc finger transcription factors. Previous crystal structures of BTB domains have revealed tightly interwound homodimers, with the N-terminus from one chain forming a two-stranded anti-parallel beta-sheet with a strand from the other chain. We have solved the crystal structures of the BTB domains from Fanconi anemia zinc finger (FAZF) and Miz1 (Myc-interacting zinc finger 1) to resolutions of 2.0 A and 2.6 A, respectively. Unlike previous examples of BTB domain structures, the FAZF BTB domain is a nonswapped dimer, with each N-terminal beta-strand associated with its own chain. As a result, the dimerization interface in the FAZF BTB domain is about half as large as in the domain-swapped dimers. The Miz1 BTB domain resembles a typical swapped BTB dimer, although it has a shorter N-terminus that is not able to form the interchain sheet. Using cysteine cross-linking, we confirmed that the promyelocytic leukemia zinc finger (PLZF) BTB dimer is strand exchanged in solution, while the FAZF BTB dimer is not. A phylogenic tree of the BTB fold based on both sequence and structural features shows that the common ancestor of the BTB domain in BTB-ZF (bric a brac, tramtrack, broad-complex zinc finger) proteins was a domain-swapped dimer. The differences in the N-termini seen in the FAZF and Miz1 BTB domains appear to be more recent developments in the structural evolution of the domain.
Insights into strand exchange in BTB domain dimers from the crystal structures of FAZF and Miz1.,Stogios PJ, Cuesta-Seijo JA, Chen L, Pomroy NC, Prive GG J Mol Biol. 2010 Jul 30;400(5):983-97. Epub 2010 May 21. PMID:20493880[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Peukert K, Staller P, Schneider A, Carmichael G, Hanel F, Eilers M. An alternative pathway for gene regulation by Myc. EMBO J. 1997 Sep 15;16(18):5672-86. PMID:9312026 doi:10.1093/emboj/16.18.5672
- ↑ Schneider A, Peukert K, Eilers M, Hanel F. Association of Myc with the zinc-finger protein Miz-1 defines a novel pathway for gene regulation by Myc. Curr Top Microbiol Immunol. 1997;224:137-46. PMID:9308237
- ↑ Basu S, Liu Q, Qiu Y, Dong F. Gfi-1 represses CDKN2B encoding p15INK4B through interaction with Miz-1. Proc Natl Acad Sci U S A. 2009 Feb 3;106(5):1433-8. doi: 10.1073/pnas.0804863106., Epub 2009 Jan 22. PMID:19164764 doi:10.1073/pnas.0804863106
- ↑ Stogios PJ, Cuesta-Seijo JA, Chen L, Pomroy NC, Prive GG. Insights into strand exchange in BTB domain dimers from the crystal structures of FAZF and Miz1. J Mol Biol. 2010 Jul 30;400(5):983-97. Epub 2010 May 21. PMID:20493880 doi:10.1016/j.jmb.2010.05.028
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