Carboxypeptidase

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'''Carboxypeptidase''' (CP) cleaves the amino acid at the C terminal of a polypeptide chain. 2-guanidinoethylmercaptosuccinic acid (GEMSA) is a potent inhibitor of CP.<br />
'''Carboxypeptidase''' (CP) cleaves the amino acid at the C terminal of a polypeptide chain. 2-guanidinoethylmercaptosuccinic acid (GEMSA) is a potent inhibitor of CP.<br />
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* '''[[Carboxypeptidase_A|Carboxypeptidase A]]''' (CPA) cleaves preferentially aromatic or branched-chain amino acids.<ref>PMID:21804935</ref><br /> For detatils see [[Carboxypeptidase A]].
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* '''[[Carboxypeptidase_A|Carboxypeptidase A]]''' (CPA) cleaves preferentially aromatic or branched-chain amino acids.<ref>PMID:21804935</ref><br />
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* '''Carboxypeptidase B''' (CPB) cleaves preferentially positively charged amino acids.<ref>PMID:15982000</ref><br />
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* '''Carboxypeptidase B''' (CPB) or '''thrombin activated fibrinolysis inhibitor''' or '''TAFIa''' cleaves preferentially positively charged amino acids.<ref>PMID:15982000</ref><br />
* '''Carboxypeptidase D''' (CPD) cleaves preferentially Arg or Lys at the C-terminal.<br />
* '''Carboxypeptidase D''' (CPD) cleaves preferentially Arg or Lys at the C-terminal.<br />
* '''Carboxypeptidase E''' (CPE) cleaves preferentially Arg or Lys at the C-terminal.<br />
* '''Carboxypeptidase E''' (CPE) cleaves preferentially Arg or Lys at the C-terminal.<br />
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* '''Carboxypeptidase M32''' is a Zn containing metalloprotease.<br />
* '''Carboxypeptidase M32''' is a Zn containing metalloprotease.<br />
* '''Glutamate carboxypeptidase''' (GCP) cleaves glutamate from the peptide N-acetyl-aspartyl-glutamate.<br />
* '''Glutamate carboxypeptidase''' (GCP) cleaves glutamate from the peptide N-acetyl-aspartyl-glutamate.<br />
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* '''Prolyl arboxypeptidase''' (PCP) cleaves preferentially C termini amino acids preceded by proline.<br />
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* '''Prolyl carboxypeptidase''' (PCP) cleaves preferentially C termini amino acids preceded by proline.<br />
 +
* '''Tubulinyl-Tyr carboxypeptidase''' or '''vasohibin''' (TTCP) removes the C-terminal Tyr of tubulin<ref>PMID:6862022</ref>.<br />
* '''D-alanyl-D-alanine carboxypeptidase''' (AACP) cross-links peptidoglycan chains is also PBP.<br />
* '''D-alanyl-D-alanine carboxypeptidase''' (AACP) cross-links peptidoglycan chains is also PBP.<br />
* '''Procarboxypeptidases''' are zymogens which undergo cleavage in order to become an active carboxypeptidase.
* '''Procarboxypeptidases''' are zymogens which undergo cleavage in order to become an active carboxypeptidase.
 +
 +
See also [[Protease]].
== Relevance ==
== Relevance ==
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== Structural highlights ==
== Structural highlights ==
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The <scene name='47/478539/Cv/2'>active site Zn+2</scene> is located at the bottom of a pocket open to the surface of the enzyme.<ref>PMID:24010887</ref>
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The <scene name='47/478539/Cv/4'>active site Zn+2</scene> is located at the bottom of a pocket open to the surface of the enzyme.<ref>PMID:24010887</ref>
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</StructureSection>
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== 3D Structures of Carboxypeptidase ==
== 3D Structures of Carboxypeptidase ==
 +
[[Carboxypeptidase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*'''Carboxypeptidase A'''
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**[[5cpa]], [[1yme]], [[1cps]], [[1ee3]], [[1cpx]], [[1m4l]] – bCPA – bovine<br />
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**[[1arl]] – bCPA <br />
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**[[1arm]], [[1eel]], [[1ell]], [[1elm]] – bCPA Zn-substituted<br />
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**[[1k9x]] – PfCPA apo – ''Pyrococcus furiosus''<br />
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**[[1ka2]], [[1ka4]] - PfCPA Zn-substituted<br />
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**[[5wvu]], [[3hoa]] – CPA – ''Thermus thermophilus''<br />
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**[[2v77]] – hCPA1 – human<br />
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**[[3dwc]] – CPA – ''Trypanosoma cruzi''<br />
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**[[3g23]] – CPA – ''Novosphingobium aromaticivorans''<br />
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**[[4ox5]] – SpCPA – ''Stretomyces pneumoniae''<br />
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**[[5e3x]] – CPA – ''Fervidobacterium islandicum''<br />
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**[[5giv]] – CPA – ''Deinococcus radiodurans''<br />
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*''CPA complex with small molecules''
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**[[3cpa]] – bCPA + glycyl-tyrosine <br />
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**[[1f57]] – bCPA + cysteine<br />
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**[[1hdq]], [[1hdu]], [[1hee]] – bCPA + phenylalanine derivative<br />
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**[[6cpa]], [[7cpa]], [[8cpa]] – bCPA + phosphate derivative<br />
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**[[2scp]], [[1cbx]], [[1bav]], [[1iy7]], [[2rfh]], [[3fx6]], [[3fvl]], [[3hlp]], [[3huv]], [[3kgq]] – bCPA + inhibitor<br />
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**[[3i1u]] – bCPA + thiirane<br />
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**[[2ctb]], [[2ctc]] – bCPA + phenyl lactate<br />
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**[[2pcu]] – hCPA4 + peptide <br />
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**[[4uee]], [[4uef]], [[4uez]] – hCPA1 + peptide inhibitor<br />
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**[[4uf4]], [[5om9]] – hCPA1 + inhibitor<br />
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**[[4oxd]] – SpCP + peptidoglycan<br />
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*''CPA complex with protein''
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**[[1zlh]] – bCPA + tick inhibitor<br />
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**[[4cpa]] – bCPA + potato inhibitor<br />
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**[[2abz]] - bCPA + metallo-CP inhibitor<br />
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**[[3fju]] – hCPA1 + ascaris inhibitor<br />
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**[[1dtd]] – hCPA2 + metallo-CP inhibitor<br />
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**[[2bo9]] – hCPA4 hydrolase domain + latexin<br />
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**[[4bd9]] - hCPA4 hydrolase domain + SMCI inhibitor<br />
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**[[4a94]] – hCPA4 + nerita versicolor inhibitor<br />
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*'''Procarboxypeptidase A'''
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**[[1fon]] – bPCPA subunit III<br />
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**[[1pyt]] – bPCPA + proproteinase E + chymotrypsinogen C<br />
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**[[1jqg]] – PCPA – Cotton bollworm<br />
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**[[1aye]] – hPCPA2 <br />
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**[[1o6x]] – hPCPA2 activation domain – NMR<BR />
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**[[2boa]] – hPCPA4<br />
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*'''Carboxypeptidase B'''
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**[[1cpb]], [[3dgv]] – bCPB <br />
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**[[3d4u]], [[3osl]] – bCPB + CP inhibitor<br />
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**[[2c1c]] – CPB – Corn earworm<br />
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**[[3glj]] – pCPB residues 16-416 - pig<br />
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**[[5jc6]] – pCPB catalytic domain residues 111-416 <br />
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**[[3wab]], [[3wc5]], [[3wc6]], [[3wc7]], [[5lyf]], [[5lyi]], [[5lyl]], [[4z65]], [[4uia]], [[2piy]], [[2piz]], [[2pj0]], [[2pj1]], [[2pj2]], [[2pj3]], [[2pj4]], [[2pj5]], [[2pj6]], [[2pj7]], [[2pj8]], [[2pj9]], [[2pja]], [[2pjb]], [[2pjc]] – pCPB catalytic domain + inhibitor<br />
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**[[5lyd]], [[4uib]] – pCPB catalytic domain (mutant) + inhibitor<br />
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**[[5lrg]], [[5lrj]], [[5lrk]] – pCPB catalytic domain + anabaenopepsin peptide<br />
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**[[3d66]] – hCPB 2<br />
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**[[3d68]] – hCPB 2 (mutant)<br />
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**[[3lms]] – hCPB 2 + protein inhibitor<br />
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**[[5hvf]], [[5hvg]], [[5hvh]] – hCPB 2 (mutant) + nanobody inhibitor<br />
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**[[4p10]] – hCPB 2 (mutant) + inhibitor<br />
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**[[3d67]] – hCPB 2 + GEMSA<br />
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**[[1zli]] - hCPB + tick inhibitor<br />
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*'''Procarboxypeptidase B'''
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**[[1pba]] – pPCPB activation domain – NMR<BR />
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**[[1nsa]] – pPCPB<br />
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**[[1z5r]] - pPCPB catalytic domain<br />
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**[[1kwm]] - hPCPB<br />
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**[[1pca]] – pPCPB + valine<br />
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**[[1zg7]], [[1zg8]], [[1zg9]] – pPCPB + inhibitor<br />
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*'''Carboxypeptidase D'''
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**[[1qmu]] – dCPD domain II - duck <br />
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**[[1h8l]] - dCPD domain II + GEMSA<br />
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**[[3mn8]] – DmCPD + GEMSA – ''Drosophila melanogaster''<br />
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**[[5aq0]] – hCPD transthyretin-like domain<br />
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*'''Dipeptidyl carboxypeptidase I'''
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**[[2iul]], [[2iux]] – hCPI (mutant) <br />
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**[[2c6f]] – hCPI N domain<br />
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**[[2x8y]] – DmCPI <br />
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**[[5l43]] – AhCPI – ''Astrosporangium hypotensionis''<br />
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*''Dipeptidyl carboxypeptidase I complexes''
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**[[2x8z]], [[2x90]], [[2x91]], [[2x92]], [[2x93]], [[2x94]], [[2x95]], [[2x96]], [[2x97]] – DmCPI + anti-hypersensitive drug<br />
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**[[2xhm]] - DmCPI + phosphate derivative<br />
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**[[2oc2]], [[2xy9]], [[2xyd]] – hCPI + phosphate derivative<br />
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**[[3nxq]] - hCPI (mutant) + phosphate derivative<br />
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**[[3bkk]], [[3bkl]], [[3l3n]] – hCPI + inhibitor<br />
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**[[2ydm]], [[3zqz]] – hCPI + captopril derivative<br />
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**[[5l44]] – AhCPI + peptide inhibitor <br />
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*'''Carboxypeptidase L'''
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**[[1ivy]]– hCPL
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*'''Carboxypeptidase M'''
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**[[1uwy]] – hCPM
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*'''Carboxypeptidase N'''
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**[[2nsm]] – hCPN catalytic domain
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*'''Carboxypeptidase O'''
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**[[5mrv]] – hCPO + metallo-CP inhibitor<br />
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*'''Carboxypeptidase T'''
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**[[1obr]], [[3qnv]] – TvCPT – ''Thermoaclinomyces vulgaris''<br />
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**[[3prt]], [[3v38]], [[4ihm]] – TvCPT (mutant)<br />
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**[[4djl]] – TvCPT + sulfamoyl phenylalanine<br />
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**[[4iav]] – TvCPT (mutant) + sulfamoyl phenylalanine<br />
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**[[5myh]] – TvCPT + sulfamoyl glutamate<br />
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**[[4duk]] – TvCPT + benzyl succinic acid<br />
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**[[4f8z]] – TvCPT + leucine derivative<br />
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**[[4ik2]] – TvCPT (mutant) + leucine derivative<br />
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**[[4gm5]] – TvCPT + arginine derivative<br />
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**[[3v7z]] – TvCPT + GEMSA<br />
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*'''Carboxypeptidase Y'''
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**[[1obr]], [[1wpx]] – yCPY + CPY inhibitor - yeast
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*'''Serine carboxypeptidase'''
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**[[3sc2]] – wSCP II – wheat<br />
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**[[1ysc]], [[1cpy]] – ySCP<br />
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**[[2j7v]] – SeSCP – ''Synechococcus elongatus''<br />
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**[[2j9o]] – SeSCP (mutant)
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*''Serine carboxypeptidase complexes''
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**[[1whs]], [[1wht]] - wSCP II + benzylsuccinate<br />
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**[[1bcr]], [[1bcs]] - wSCP II + antipain + arginine<br />
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**[[2j8y]] – SeSCP + penicillin<br />
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**[[2jbf]] - SeSCP (mutant) + penicillin
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*'''Arginine carboxypeptidase'''
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**[[3be7]], [[3dug]] – RCP<br />
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**[[3mtw]] – RCP + arginine derivative – ''Caulobacter vibrioides''
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*'''D-alanyl-D-alanine carboxypeptidase'''
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**[[1w79]], [[1w8q]] – AACP – ''Actinomadura''<br />
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**[[3pte]], [[1skf]] – StAACP – ''Streptomyces''<br />
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**[[4rye]] – MtAACP <br />
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**[[5fsr]] – EcAACP <br />
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**[[1es2]], [[1es3]], [[1es4]], [[1es5]], [[1esi]], [[1j9m]] – StAACP (mutant) <br />
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**[[1lbu]] – SaAACP + Zn – ''Streptomyces albus''<br />
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**[[2wke]], [[1w8y]], [[2vgj]], [[2vgk]], [[4ben]] - AcAACP + antibiotic<br />
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**[[1cef]], [[1ceg]], [[1hvb]], [[1pw1]], [[1pw8]], [[1pwc]], [[1pwd]], **[[1pwg]] – StAACP + antibiotic<br />
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**[[2xdm]], [[2xk1]], [[2xln]], [[2y4a]], [[2y55]], [[2y59]], [[3zvt]], [[3zvw]], [[4b4x]], [[4b4z]] – AcAACP + boronic acid derivative<br />
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**[[1ikg]], [[1iki]] – StAACP + peptidoglycan fragment<br />
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**[[1mpl]] - StAACP + phosphonate inhibitor<br />
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**[[1scw]], [[1sde]] - StAACP + cyclic phosphate inhibitor<br />
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**[[1yqs]] - StAACP + sultam inhibitor<br />
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**[[3zcz]] – AcAACP + inhibitor<br />
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**[[5j8x]] – EcAACP + inhibitor<br />
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*β-lactamase/D-alanine carboxypeptidase
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**[[5evi]] - LACP – ''Pseudomonas syringae''<br />
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**[[3ozh]], [[3rju]] – ACP – ''Yersinia pestis''<br />
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*'''Glutamate carboxypeptidase II'''
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**[[1z8l]], [[2oot]] – hECP II<br />
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**[[2c6c]], [[2jbj]], [[2jbk]], [[2or4]], [[3d7d]], [[3d7f]], [[3d7g]], [[3d7h]], [[3iww]], [[3sjf]], [[4jyw]], [[4jz0]], [[4lqg]], [[5ely]], [[5d29]], [[4x3r]], [[4w9y]] – hECP II + inhibitor<br />
+
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**[[4p44]], [[4p45]], [[4p4e]], [[4p4d]], [[4p4f]], [[4p4i]], [[4p4j]], [[4p4b]] – hECP II + phosphoramidate inhibitor<br />
+
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**[[4ngm]], [[4ngn]], [[4ngp]], [[4ngq]], [[4ngr]], [[4ngs]], [[4ngt]], [[4oc0]], [[4oc1]], [[4oc2]], [[4oc3]], [[4oc4]], [[4oc5]], [[4ome]] - hECP II + urea derivative<br />
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**[[2c6g]] - hECP II + glutamate<br />
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**[[4mcr]], [[4mcs]] - hECP II (mutant) + glutamate<br />
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**[[4mcp]], [[4mcq]], [[5f09]] - hECP II (mutant) + glutamate derivative<br />
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**[[3bhx]], [[3bi0]], [[3bi1]] - hECP II + transition state analog<br />
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**[[2cij]] – hECP II + methionine<br />
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**[[2pvv]] - hECP II + serine-sulfate<br />
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**[[2c6p]], [[2pvw]] - hECP II + phosphate derivative<br />
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**[[3rbu]] – hECP II + PMPA<br />
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**[[3bxm]] – hECP II (mutant) + dipeptide<br />
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**[[3sje]], [[3sjg]], [[3sjx]] - hECP II (mutant) + inhibitor<br />
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**[[2xef]], [[2xeg]], [[2xei]], [[2xej]] – hECP II + antibody-recruiting molecule<br />
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**[[1cg2]] – ECP II – ''Pseudomonas''
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-
 
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*'''Glutamate carboxypeptidase III'''
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**[[3fec]] – hECP III<br />
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**[[3fed]] - hECP III + transition state analog<br />
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**[[3fee]] – hECP III + inhibitor<br />
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**[[3ff3]] - hECP III + glutamate
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-
 
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*'''Prolyl carboxypeptidase'''
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**[[3n2z]] - hPCP
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*'''Angiotensin-converting enzyme-related carboxypeptidase'''
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See [[Angiotensin-Converting Enzyme]]
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*'''M32 carboxypeptidase'''
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**[[3hq2]] – BsCP
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-
 
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*'''Muramoyl pentapeptide carboxypeptidase'''
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**[[1lbu]] – SaCP<br />
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-
 
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*'''Metallo-carboxypeptidase'''
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**[[4a37]] – PaCP – ''Pseudomonas aeruginosa''<br />
 
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**[[4a38]] – PaCP + benzyl succinic acid<br />
 
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**[[4a39]] – PaCP + GEMSA<br />
 
-
}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Pig carboxypeptidase B complex with acetate and Zn+2 ions (grey) (PDB code 3wc6)

Drag the structure with the mouse to rotate


References

  1. ↑ Fernandez D, Boix E, Pallares I, Aviles FX, Vendrell J. Structural and Functional Analysis of the Complex between Citrate and the Zinc Peptidase Carboxypeptidase A. Enzyme Res. 2011;2011:128676. doi: 10.4061/2011/128676. Epub 2011 Jul 25. PMID:21804935 doi:10.4061/2011/128676
  2. ↑ Adler M, Bryant J, Buckman B, Islam I, Larsen B, Finster S, Kent L, May K, Mohan R, Yuan S, Whitlow M. Crystal structures of potent thiol-based inhibitors bound to carboxypeptidase B. Biochemistry. 2005 Jul 5;44(26):9339-47. PMID:15982000 doi:http://dx.doi.org/10.1021/bi0501941
  3. ↑ Arce CA, Barra HS. Association of tubulinyl-tyrosine carboxypeptidase with microtubules. FEBS Lett. 1983 Jun 27;157(1):75-8. doi: 10.1016/0014-5793(83)81119-3. PMID:6862022 doi:http://dx.doi.org/10.1016/0014-5793(83)81119-3
  4. ↑ Yoshimoto N, Itoh T, Inaba Y, Ishii H, Yamamoto K. Structural Basis for Inhibition of Carboxypeptidase B by Selenium-Containing Inhibitor: Selenium Coordinates to Zinc in Enzyme. J Med Chem. 2013 Sep 24. PMID:24010887 doi:10.1021/jm400816v

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