Carbamoyl phosphate synthetase
From Proteopedia
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== Function == | == Function == | ||
| - | '''Carbamoyl phosphate synthetase''' (CPS) catalyzes the production of carbamoyl phosphate from ATP, Mg+2, bicarbonate | + | '''Carbamoyl phosphate synthetase''' (CPS) catalyzes the production of carbamoyl phosphate from ATP, Mg+2, bicarbonate and glutamine. CPS is part of the pyrimidine and arginine biosynthesis as well as the urea cycle in vertebrates. Ornithine is an allosteric effector of CPS.<ref>PMID:5329589</ref> There are 3 forms of CPS:<br /> |
* '''CPS I''' participates in the urea cycle. CPS I uses glutamine for NH3 source.<br /> | * '''CPS I''' participates in the urea cycle. CPS I uses glutamine for NH3 source.<br /> | ||
* '''CPS II''' participates in pyrimidine metabolism<br /> | * '''CPS II''' participates in pyrimidine metabolism<br /> | ||
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== Structural highlights == | == Structural highlights == | ||
| - | CPS I and II are composed of 2 subunits. The large subunit contain active sites which bind nucleotides and other effectors. The small subunit catalyzes the hydrolysis of glutamine to glutamate and NH3. The <scene name='49/493669/Cv/3'>small subunit active site contains an active Cys residue</scene>.<ref>PMID:9636022</ref> CPS I contains a methylglyoxal synthetase (MGS) domain which binds ornithine | + | CPS I and II are composed of 2 subunits. The large subunit contain active sites which bind nucleotides and other effectors. The small subunit catalyzes the hydrolysis of glutamine to glutamate and NH3. The <scene name='49/493669/Cv/3'>small subunit active site contains an active Cys residue</scene>.<ref>PMID:9636022</ref> CPS I contains a methylglyoxal synthetase (MGS) domain which binds <scene name='49/493669/Cv/6'>L-ornithine</scene>. Water molecules are shown as red spheres. |
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| - | + | <scene name='49/493669/Cv/11'>ADP binding site</scene>. | |
| - | + | <scene name='49/493669/Cv/12'>Mn coordination site</scene>. | |
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| - | + | <scene name='49/493669/Cv/13'>K coordination site</scene>. | |
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| - | + | ==3D structures of carbamoyl phosphate synthetase== | |
| - | + | [[Carbamoyl phosphate synthetase 3D structures]] | |
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| - | [[ | + | </StructureSection> |
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== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Kalman SM, Duffield PH, Brzozowski T. Purification and properties of a bacterial carbamyl phosphate synthetase. J Biol Chem. 1966 Apr 25;241(8):1871-7. PMID:5329589
- ↑ Thoden JB, Miran SG, Phillips JC, Howard AJ, Raushel FM, Holden HM. Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis. Biochemistry. 1998 Jun 23;37(25):8825-31. PMID:9636022 doi:http://dx.doi.org/10.1021/bi9807761
