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| - | [[Image:4hb1.jpg|left|200px]] | |
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| - | {{Structure
| + | ==A DESIGNED FOUR HELIX BUNDLE PROTEIN.== |
| - | |PDB= 4hb1 |SIZE=350|CAPTION= <scene name='initialview01'>4hb1</scene>, resolution 2.9Å
| + | <StructureSection load='4hb1' size='340' side='right'caption='[[4hb1]], [[Resolution|resolution]] 2.90Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene> | + | <table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HB1 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | |GENE= SYNTHETIC GENE DHP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 synthetic construct])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [https://pdbe.org/4hb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [https://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hb1 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [http://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''A DESIGNED FOUR HELIX BUNDLE PROTEIN.'''
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| - | | + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | A 108 amino acid protein was designed and constructed from a reduced alphabet of seven amino acids. The 2.9 A resolution X-ray crystal structure confirms that the protein is a four helix bundle, as it was designed to be. Hydrogen/deuterium exchange experiments reveal buried amide protons with protection factors in excess of 1 x 10(6) in the range characteristic of well protected protons in functional folded proteins (10(3)-10(8)) rather than protons in rapid exchange (0-10(2)). The protein is monomeric at 1 mM, the concentration at which the exchange experiments were undertaken, indicating that the exchange factors are due to a unique stable tertiary structure fold, and not due to any higher order quaternary structure. Thermodynamic analysis provides an estimate of the free energy of folding of -9.3 kcal mole-1 at 25 degrees C, consistent with the free energy of folding derived from the protection factors of the most protected protons, indicating that global unfolding is required for exchange of the most protected protons.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 4HB1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | A designed four helix bundle protein with native-like structure., Schafmeister CE, LaPorte SL, Miercke LJ, Stroud RM, Nat Struct Biol. 1997 Dec;4(12):1039-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9406555 9406555]
| + | |
| - | [[Category: Protein complex]] | + | |
| | [[Category: Synthetic construct]] | | [[Category: Synthetic construct]] |
| - | [[Category: Laporte, S L.]] | + | [[Category: Laporte SL]] |
| - | [[Category: Miercke, L J.W.]] | + | [[Category: Miercke LJW]] |
| - | [[Category: Schafmeister, C E.]] | + | [[Category: Schafmeister CE]] |
| - | [[Category: Stroud, R M.]] | + | [[Category: Stroud RM]] |
| - | [[Category: designed helical bundle]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:39:03 2008''
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