5eas

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[[Image:5eas.gif|left|200px]]
 
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{{Structure
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==5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM==
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|PDB= 5eas |SIZE=350|CAPTION= <scene name='initialview01'>5eas</scene>, resolution 2.25&Aring;
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<StructureSection load='5eas' size='340' side='right'caption='[[5eas]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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|SITE= <scene name='pdbsite=MGA:Mg2++Binding+Site'>MGA</scene> and <scene name='pdbsite=MGB:Mg2++Binding+Site'>MGB</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[5eas]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EAS FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eas OCA], [https://pdbe.org/5eas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eas RCSB], [https://www.ebi.ac.uk/pdbsum/5eas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eas ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eas OCA], [http://www.ebi.ac.uk/pdbsum/5eas PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=5eas RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/5EAS_TOBAC 5EAS_TOBAC] Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic intermediate 5-epi-aristolochene, initial step in the conversion of FPP to the sesquiterpenoid antifungal phytoalexin capsidiol. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce the bicyclic 5-epi-aristolochene.
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== Evolutionary Conservation ==
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'''5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/5eas_consurf.spt"</scriptWhenChecked>
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Terpene cyclases catalyze the synthesis of cyclic terpenes with 10-, 15-, and 20-carbon acyclic isoprenoid diphosphates as substrates. Plants have been a source of these natural products by providing a homologous set of terpene synthases. The crystal structures of 5-epi-aristolochene synthase, a sesquiterpene cyclase from tobacco, alone and complexed separately with two farnesyl diphosphate analogs were analyzed. These structures reveal an unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the stereochemical selectivity displayed by other cyclases in the biosynthesis of pharmacologically important cyclic terpenes. As such, these structures provide templates for the engineering of novel terpene cyclases.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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5EAS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EAS OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=5eas ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase., Starks CM, Back K, Chappell J, Noel JP, Science. 1997 Sep 19;277(5333):1815-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9295271 9295271]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Nicotiana tabacum]]
[[Category: Nicotiana tabacum]]
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[[Category: Single protein]]
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[[Category: Back K]]
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[[Category: Back, K.]]
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[[Category: Chappell J]]
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[[Category: Chappell, J.]]
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[[Category: Noel JP]]
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[[Category: Noel, J P.]]
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[[Category: Starks CM]]
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[[Category: Starks, C M.]]
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[[Category: 5-epi-aristolochene synthase]]
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[[Category: isoprenoid biosynthesis]]
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[[Category: isoprenoid cyclase]]
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[[Category: isoprenoid synthase]]
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[[Category: natural products biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:41:17 2008''
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Current revision

5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM

PDB ID 5eas

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