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| | ==Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)== | | ==Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)== |
| - | <StructureSection load='3n0f' size='340' side='right' caption='[[3n0f]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='3n0f' size='340' side='right'caption='[[3n0f]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3n0f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Grey_poplar Grey poplar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N0F FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3n0f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_tremula_x_Populus_alba Populus tremula x Populus alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N0F FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3n0g|3n0g]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ispS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=80863 Grey poplar])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0f OCA], [https://pdbe.org/3n0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n0f RCSB], [https://www.ebi.ac.uk/pdbsum/3n0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n0f ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isoprene_synthase Isoprene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.27 4.2.3.27] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0f OCA], [http://pdbe.org/3n0f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n0f RCSB], [http://www.ebi.ac.uk/pdbsum/3n0f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n0f ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ISPS_POPCN ISPS_POPCN] Lyase that catalyzes the formation of isoprene from dimethylallyl diphosphate via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base.<ref>PMID:11506373</ref> <ref>PMID:20624401</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Grey poplar]] | + | [[Category: Large Structures]] |
| - | [[Category: Isoprene synthase]] | + | [[Category: Populus tremula x Populus alba]] |
| - | [[Category: Christianson, D W]] | + | [[Category: Christianson DW]] |
| - | [[Category: Koksal, M]] | + | [[Category: Koksal M]] |
| - | [[Category: Ddxxd motif]]
| + | |
| - | [[Category: Hemiterpene synthase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Nse/dte motif]]
| + | |
| - | [[Category: Terpene cyclase fold]]
| + | |
| Structural highlights
Function
ISPS_POPCN Lyase that catalyzes the formation of isoprene from dimethylallyl diphosphate via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The X-ray crystal structure of recombinant PcISPS (isoprene synthase from gray poplar hybrid Populusxcanescens) has been determined at 2.7 A resolution, and the structure of its complex with three Mg(2+) and the unreactive substrate analogue dimethylallyl-S-thiolodiphosphate has been determined at 2.8 A resolution. Analysis of these structures suggests that the generation of isoprene from substrate dimethylallyl diphosphate occurs via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base. This chemical mechanism is responsible for the annual atmospheric emission of 100 Tg of isoprene by terrestrial plant life. Importantly, the PcISPS structure promises to guide future protein engineering studies, potentially leading to hydrocarbon fuels and products that do not rely on traditional petrochemical sources.
Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission.,Koksal M, Zimmer I, Schnitzler JP, Christianson DW J Mol Biol. 2010 Jul 17. PMID:20624401[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Miller B, Oschinski C, Zimmer W. First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli. Planta. 2001 Jul;213(3):483-7. PMID:11506373 doi:10.1007/s004250100557
- ↑ Koksal M, Zimmer I, Schnitzler JP, Christianson DW. Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission. J Mol Biol. 2010 Jul 17. PMID:20624401 doi:10.1016/j.jmb.2010.07.009
- ↑ Koksal M, Zimmer I, Schnitzler JP, Christianson DW. Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission. J Mol Biol. 2010 Jul 17. PMID:20624401 doi:10.1016/j.jmb.2010.07.009
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