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3mqk

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Cbf5-Nop10-Gar1 complex binding with 17mer RNA containing ACA trinucleotide

Structural highlights

3mqk is a 5 chain structure with sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TRUB_PYRFU Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs (By similarity).

Publication Abstract from PubMed

Cbf5 is the catalytic subunit of the H/ACA small nucleolar/Cajal body ribonucleoprotein particles (RNPs) responsible for site specific isomerization of uridine in ribosomal and small nuclear RNA. Recent evidence from studies on archaeal Cbf5 suggests its second functional role in modifying tRNA U55 independent of guide RNA. In order to act both as a stand-alone and a RNP pseudouridine synthase, Cbf5 must differentiate features in H/ACA RNA from those in tRNA or rRNA. Most H/ACA RNAs contain a hallmark ACA trinucleotide downstream of the H/ACA motif. Here we challenged an archaeal Cbf5 (in the form of a ternary complex with its accessory proteins Nop10 and Gar1) with T-stem-loop RNAs with or without ACA trinucleotide in the stem. Although these substrates were previously shown to be substrates for the bacterial stand-alone pseudouridine synthase TruB, the Cbf5-Nop10-Gar1 complex was only able to modify those without ACA trinucleotide. A crystal structure of Cbf5-Nop10-Gar1 trimer bound with an ACA-containing T-stem-loop revealed that the ACA trinucleotide detracted Cbf5 from the stand-alone binding mode, thereby suggesting that the H/ACA RNP-associated function of Cbf5 likely supersedes its stand-alone function.

Structural and functional evidence of high specificity of Cbf5 for ACA trinucleotide.,Zhou J, Liang B, Li H RNA. 2010 Dec 13. PMID:21149572^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhou J, Liang B, Li H. Structural and functional evidence of high specificity of Cbf5 for ACA trinucleotide. RNA. 2010 Dec 13. PMID:21149572 doi:10.1261/rna.2415811

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3mqk"

Categories: Large Structures | Pyrococcus furiosus | Li H | Liang B | Zhou J

@@ Line 1: / Line 1: @@
 ==Cbf5-Nop10-Gar1 complex binding with 17mer RNA containing ACA trinucleotide==
-<StructureSection load='3mqk' size='340' side='right' caption='[[3mqk]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='3mqk' size='340' side='right'caption='[[3mqk]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3mqk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MQK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3mqk]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MQK FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">truB, PF1785 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 ATCC 43587]), PF1141 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 ATCC 43587]), PF1791 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 ATCC 43587])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mqk OCA], [http://pdbe.org/3mqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mqk RCSB], [http://www.ebi.ac.uk/pdbsum/3mqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mqk ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mqk OCA], [https://pdbe.org/3mqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mqk RCSB], [https://www.ebi.ac.uk/pdbsum/3mqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mqk ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TRUB_PYRFU TRUB_PYRFU]] Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs (By similarity). [[http://www.uniprot.org/uniprot/NOP10_PYRFU NOP10_PYRFU]] Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA (By similarity).
+[https://www.uniprot.org/uniprot/TRUB_PYRFU TRUB_PYRFU] Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 ==See Also==
 *[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
-*[[Ribosome biogenesis protein|Ribosome biogenesis protein]]
+*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
+*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Atcc 43587]]
+[[Category: Large Structures]]
-[[Category: Li, H]]
+[[Category: Pyrococcus furiosus]]
-[[Category: Liang, B]]
+[[Category: Li H]]
-[[Category: Zhou, J]]
+[[Category: Liang B]]
-[[Category: Box h/aca]]
+[[Category: Zhou J]]
-[[Category: Isomerase]]
-[[Category: Isomerase-rna binding protein-rna complex]]
-[[Category: Post-transcriptional modification]]
-[[Category: Protein-rna complex]]
-[[Category: Pseudouridine synthase]]
-[[Category: Rna-binding]]
-[[Category: Trna processing]]

3mqk

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Current revision

Cbf5-Nop10-Gar1 complex binding with 17mer RNA containing ACA trinucleotide

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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