6j6i

From Proteopedia

(Difference between revisions)

Current revision

Reconstitution and structure of a plant NLR resistosome conferring immunity

6j6i, resolution 3.70Å

Structural highlights

6j6i is a 7 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PBL2_ARATH Involved in disease resistance signaling (PubMed:20413097, PubMed:23951354). Contributes to pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling and defense responses downstream of FLS2 (PubMed:20413097). Required for the bacterial XopAC/AvrAC effector-triggered immunity (ETI) against Xanthomonas campestris (PubMed:23951354).^[1] ^[2]

Publication Abstract from PubMed

Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. Biochemical mechanisms underlying plant NLR activation have until now remained poorly understood. We reconstituted an active complex containing the Arabidopsis coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an alpha-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic alpha helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death triggering, and disease resistance, offering clues to the biochemical function of a plant resistosome.

Reconstitution and structure of a plant NLR resistosome conferring immunity.,Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW, Zhou JM, Chai J Science. 2019 Apr 5;364(6435). pii: 364/6435/eaav5870. doi:, 10.1126/science.aav5870. PMID:30948527^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang J, Li W, Xiang T, Liu Z, Laluk K, Ding X, Zou Y, Gao M, Zhang X, Chen S, Mengiste T, Zhang Y, Zhou JM. Receptor-like cytoplasmic kinases integrate signaling from multiple plant immune receptors and are targeted by a Pseudomonas syringae effector. Cell Host Microbe. 2010 Apr 22;7(4):290-301. doi: 10.1016/j.chom.2010.03.007. PMID:20413097 doi:http://dx.doi.org/10.1016/j.chom.2010.03.007
↑ Guy E, Lautier M, Chabannes M, Roux B, Lauber E, Arlat M, Noel LD. xopAC-triggered immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK. PLoS One. 2013 Aug 9;8(8):e73469. doi: 10.1371/journal.pone.0073469. eCollection , 2013. PMID:23951354 doi:http://dx.doi.org/10.1371/journal.pone.0073469
↑ Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW, Zhou JM, Chai J. Reconstitution and structure of a plant NLR resistosome conferring immunity. Science. 2019 Apr 5;364(6435). pii: 364/6435/eaav5870. doi:, 10.1126/science.aav5870. PMID:30948527 doi:http://dx.doi.org/10.1126/science.aav5870

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6j6i"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6j6i is ON HOLD
+==Reconstitution and structure of a plant NLR resistosome conferring immunity==
+<SX load='6j6i' size='340' side='right' viewer='molstar' caption='[[6j6i]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6j6i]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J6I FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTP:2-DEOXYADENOSINE+5-TRIPHOSPHATE'>DTP</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j6i OCA], [https://pdbe.org/6j6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j6i RCSB], [https://www.ebi.ac.uk/pdbsum/6j6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j6i ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PBL2_ARATH PBL2_ARATH] Involved in disease resistance signaling (PubMed:20413097, PubMed:23951354). Contributes to pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling and defense responses downstream of FLS2 (PubMed:20413097). Required for the bacterial XopAC/AvrAC effector-triggered immunity (ETI) against Xanthomonas campestris (PubMed:23951354).<ref>PMID:20413097</ref> <ref>PMID:23951354</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. Biochemical mechanisms underlying plant NLR activation have until now remained poorly understood. We reconstituted an active complex containing the Arabidopsis coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an alpha-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic alpha helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death triggering, and disease resistance, offering clues to the biochemical function of a plant resistosome.
-Authors: Jizong, W., jia, W., Meijuan, H., Hongwei, W., Jianmin, Z., Jijie, C.
+Reconstitution and structure of a plant NLR resistosome conferring immunity.,Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW, Zhou JM, Chai J Science. 2019 Apr 5;364(6435). pii: 364/6435/eaav5870. doi:, 10.1126/science.aav5870. PMID:30948527<ref>PMID:30948527</ref>
-Description: Reconstitution and structure of a plant NLR resistosome conferring immunity
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Jianmin, Z]]
+<div class="pdbe-citations 6j6i" style="background-color:#fffaf0;"></div>
-[[Category: Jizong, W]]
+== References ==
-[[Category: Jia, W]]
+<references/>
-[[Category: Jijie, C]]
+__TOC__
-[[Category: Meijuan, H]]
+</SX>
-[[Category: Hongwei, W]]
+[[Category: Arabidopsis thaliana]]
+[[Category: Large Structures]]
+[[Category: Chai JJ]]
+[[Category: Hu MJ]]
+[[Category: Wang HW]]
+[[Category: Wang J]]
+[[Category: Wang JZ]]
+[[Category: Zhou JM]]

6j6i

From Proteopedia

Current revision

Reconstitution and structure of a plant NLR resistosome conferring immunity

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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