6njq
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of TBP-Hoogsteen containing DNA complex== | |
| + | <StructureSection load='6njq' size='340' side='right'caption='[[6njq]], [[Resolution|resolution]] 2.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6njq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NJQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6njq OCA], [https://pdbe.org/6njq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6njq RCSB], [https://www.ebi.ac.uk/pdbsum/6njq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6njq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/TBP1_ARATH TBP1_ARATH] General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hoogsteen DNA base pairs (bps) are an alternative base pairing to canonical Watson-Crick bps and are thought to play important biochemical roles. Hoogsteen bps have been reported in a handful of X-ray structures of protein-DNA complexes. However, there are several examples of Hoogsteen bps in crystal structures that form Watson-Crick bps when examined under solution conditions. Furthermore, Hoogsteen bps can sometimes be difficult to resolve in DNA:protein complexes by X-ray crystallography due to ambiguous electron density and by solution-state NMR spectroscopy due to size limitations. Here, using infrared spectroscopy, we report the first direct solution-state observation of a Hoogsteen (G-C(+) ) bp in a DNA:protein complex under solution conditions with specific application to DNA-bound TATA-box binding protein. These results support a previous assignment of a G-C(+) Hoogsteen bp in the complex, and indicate that Hoogsteen bps do indeed exist under solution conditions in DNA:protein complexes. | ||
| - | + | Infrared Spectroscopic Observation of a G-C(+) Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions.,Stelling AL, Liu AY, Zeng W, Salinas R, Schumacher MA, Al-Hashimi HM Angew Chem Int Ed Engl. 2019 Aug 26;58(35):12010-12013. doi:, 10.1002/anie.201902693. Epub 2019 Jul 25. PMID:31268220<ref>PMID:31268220</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6njq" style="background-color:#fffaf0;"></div> |
| - | [[Category: Schumacher | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Arabidopsis thaliana]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Schumacher MA]] | ||
| + | [[Category: Stelling A]] | ||
Current revision
Structure of TBP-Hoogsteen containing DNA complex
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