8icd

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[[Image:8icd.gif|left|200px]]
 
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{{Structure
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==REGULATION OF AN ENZYME BY PHOSPHORYLATION AT THE ACTIVE SITE==
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|PDB= 8icd |SIZE=350|CAPTION= <scene name='initialview01'>8icd</scene>, resolution 2.5&Aring;
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<StructureSection load='8icd' size='340' side='right'caption='[[8icd]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ICT:ISOCITRIC+ACID'>ICT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[8icd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The September 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Isocitrate Dehydrogenase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_9 10.2210/rcsb_pdb/mom_2010_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ICD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ICD FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ICT:ISOCITRIC+ACID'>ICT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8icd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8icd OCA], [https://pdbe.org/8icd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8icd RCSB], [https://www.ebi.ac.uk/pdbsum/8icd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8icd ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8icd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8icd OCA], [http://www.ebi.ac.uk/pdbsum/8icd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=8icd RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/IDH_ECOLI IDH_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ic/8icd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=8icd ConSurf].
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<div style="clear:both"></div>
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'''REGULATION OF AN ENZYME BY PHOSPHORYLATION AT THE ACTIVE SITE'''
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==See Also==
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*[[Isocitrate dehydrogenase 3D structures|Isocitrate dehydrogenase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The isocitrate dehydrogenase of Escherichia coli is an example of a ubiquitous class of enzymes that are regulated by covalent modification. In the three-dimensional structure of the enzyme-substrate complex, isocitrate forms a hydrogen bond with Ser113, the site of regulatory phosphorylation. The structures of Asp113 and Glu113 mutants, which mimic the inactivation of the enzyme by phosphorylation, show minimal conformational changes from wild type, as in the phosphorylated enzyme. Calculations based on observed structures suggest that the change in electrostatic potential when a negative charge is introduced either by phosporylation or site-directed mutagenesis is sufficient to inactivate the enzyme. Thus, direct interaction at a ligand binding site is an alternative mechanism to induced conformational changes from an allosteric site in the regulation of protein activity by phosphorylation.
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==About this Structure==
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8ICD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ICD OCA].
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==Reference==
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Regulation of an enzyme by phosphorylation at the active site., Hurley JH, Dean AM, Sohl JL, Koshland DE Jr, Stroud RM, Science. 1990 Aug 31;249(4972):1012-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2204109 2204109]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Isocitrate dehydrogenase (NADP(+))]]
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[[Category: Isocitrate Dehydrogenase]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dean, A M.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Hurley, J H.]]
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[[Category: Dean AM]]
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[[Category: Koshlandjunior, D E.]]
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[[Category: Hurley JH]]
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[[Category: Sohl, J L.]]
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[[Category: Koshlandjunior DE]]
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[[Category: Stroud, R M.]]
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[[Category: Sohl JL]]
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[[Category: oxidoreductase (nad(a)-choh(d))]]
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[[Category: Stroud RM]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:45:16 2008''
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Current revision

REGULATION OF AN ENZYME BY PHOSPHORYLATION AT THE ACTIVE SITE

PDB ID 8icd

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