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6nqk
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6nqk is ON HOLD Authors: Lin, C.-Y., Romei, M.G., Mathews, I.I., Boxer, S.G. Description: Crystal structure of fast switching M159T mutant of fluor...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2), Y63(3-FY)== | |
| + | <StructureSection load='6nqk' size='340' side='right'caption='[[6nqk]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6nqk]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NQK FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KZV:{(4Z)-2-[(1R)-1-amino-2-sulfanylethyl]-4-[(3-fluoro-4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}ace+tic+acid'>KZV</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nqk OCA], [https://pdbe.org/6nqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nqk RCSB], [https://www.ebi.ac.uk/pdbsum/6nqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nqk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rotation around a specific bond after photoexcitation is central to vision and emerging opportunities in optogenetics, super-resolution microscopy, and photoactive molecular devices. Competing roles for steric and electrostatic effects that govern bond-specific photoisomerization have been widely discussed, the latter originating from chromophore charge transfer upon excitation. We systematically altered the electrostatic properties of the green fluorescent protein chromophore in a photoswitchable variant, Dronpa2, using amber suppression to introduce electron-donating and electron-withdrawing groups to the phenolate ring. Through analysis of the absorption (color), fluorescence quantum yield, and energy barriers to ground- and excited-state isomerization, we evaluate the contributions of sterics and electrostatics quantitatively and demonstrate how electrostatic effects bias the pathway of chromophore photoisomerization, leading to a generalized framework to guide protein design. | ||
| - | + | Electrostatic control of photoisomerization pathways in proteins.,Romei MG, Lin CY, Mathews II, Boxer SG Science. 2020 Jan 3;367(6473):76-79. doi: 10.1126/science.aax1898. PMID:31896714<ref>PMID:31896714</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6nqk" style="background-color:#fffaf0;"></div> |
| - | [[Category: Boxer | + | |
| - | [[Category: Mathews | + | ==See Also== |
| - | [[Category: Romei | + | *[[Dronpa|Dronpa]] |
| + | *[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Echinophyllia sp. SC22]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Boxer SG]] | ||
| + | [[Category: Lin C-Y]] | ||
| + | [[Category: Mathews II]] | ||
| + | [[Category: Romei MG]] | ||
Current revision
Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2), Y63(3-FY)
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