8mht

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (18:18, 20 September 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:8mht.gif|left|200px]]
 
-
{{Structure
+
==CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX==
-
|PDB= 8mht |SIZE=350|CAPTION= <scene name='initialview01'>8mht</scene>, resolution 2.76&Aring;
+
<StructureSection load='8mht' size='340' side='right'caption='[[8mht]], [[Resolution|resolution]] 2.76&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=DU:2&#39;-DEOXYURIDINE-5&#39;-MONOPHOSPHATE'>DU</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
+
<table><tr><td colspan='2'>[[8mht]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8MHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8MHT FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.76&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8mht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8mht OCA], [https://pdbe.org/8mht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8mht RCSB], [https://www.ebi.ac.uk/pdbsum/8mht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8mht ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8mht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8mht OCA], [http://www.ebi.ac.uk/pdbsum/8mht PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=8mht RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mh/8mht_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=8mht ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Three structures have been determined for complexes between HhaI methyltransferase (M.HhaI) and oligonucleotides containing a G:A, G:U or G:AP (AP = abasic or apurinic/apyrimidinic) mismatch at the target base pair. The mismatched adenine, uracil and abasic site are all flipped out of the DNA helix and located in the enzyme's active-site pocket, adopting the same conformation as in the flipped-out normal substrate. These results, particularly the flipped-out abasic deoxyribose sugar, provide insight into the mechanism of base flipping. If the process involves the protein pushing the base out of the helix, then the push must take place not on the base, but rather on the sugar-phosphate backbone. Thus rotation of the DNA backbone is probably the key to base flipping.
-
'''CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX'''
+
Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base.,O'Gara M, Horton JR, Roberts RJ, Cheng X Nat Struct Biol. 1998 Oct;5(10):872-7. PMID:9783745<ref>PMID:9783745</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 8mht" style="background-color:#fffaf0;"></div>
-
==Overview==
+
==See Also==
-
Three structures have been determined for complexes between HhaI methyltransferase (M.HhaI) and oligonucleotides containing a G:A, G:U or G:AP (AP = abasic or apurinic/apyrimidinic) mismatch at the target base pair. The mismatched adenine, uracil and abasic site are all flipped out of the DNA helix and located in the enzyme's active-site pocket, adopting the same conformation as in the flipped-out normal substrate. These results, particularly the flipped-out abasic deoxyribose sugar, provide insight into the mechanism of base flipping. If the process involves the protein pushing the base out of the helix, then the push must take place not on the base, but rather on the sugar-phosphate backbone. Thus rotation of the DNA backbone is probably the key to base flipping.
+
*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
8MHT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8MHT OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
-
Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base., O'Gara M, Horton JR, Roberts RJ, Cheng X, Nat Struct Biol. 1998 Oct;5(10):872-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9783745 9783745]
+
-
[[Category: Deleted entry]]
+
[[Category: Haemophilus haemolyticus]]
[[Category: Haemophilus haemolyticus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Cheng, X.]]
+
[[Category: Cheng X]]
-
[[Category: Gara, M O.]]
+
[[Category: Horton JR]]
-
[[Category: Horton, J R.]]
+
[[Category: O'Gara M]]
-
[[Category: Roberts, R J.]]
+
[[Category: Roberts RJ]]
-
[[Category: complex (methyltransferase/ dna)]]
+
-
[[Category: methyltransferase]]
+
-
[[Category: restriction system]]
+
-
[[Category: transferase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:45:34 2008''
+

Current revision

CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX

PDB ID 8mht

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8mht"
Personal tools