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6qaz
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of gp41-1 intein== | |
| + | <StructureSection load='6qaz' size='340' side='right'caption='[[6qaz]], [[Resolution|resolution]] 1.02Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6qaz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metagenome Metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QAZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.02Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qaz OCA], [https://pdbe.org/6qaz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qaz RCSB], [https://www.ebi.ac.uk/pdbsum/6qaz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qaz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Protein trans-splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 A-resolution crystal structure of a fused variant from the naturally split gp41-1 intein, previously identified from environmental metagenomic sequence data. The structure of the 125-residue gp41-1 intein revealed a compact pseudo-C2-symmetry commonly found in the Hedgehog/Intein (HINT) superfamily with extensive charge-charge interactions between the split N- and C-terminal intein fragments that are common among naturally occurring split inteins. We successfully created orthogonal split inteins by engineering a similar charge network into the same region of a cis-splicing intein. This strategy could be applicable for creating novel natural-like split inteins from other, more prevalent cis-splicing inteins. | ||
| - | + | The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins.,Michael Beyer H, Malgorzata Mikula K, Li M, Wlodawer A, Iwai H FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. PMID:31665813<ref>PMID:31665813</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6qaz" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Metagenome]] | ||
| + | [[Category: Beyer HM]] | ||
| + | [[Category: Iwai H]] | ||
| + | [[Category: Li M]] | ||
| + | [[Category: Mikula KM]] | ||
| + | [[Category: Wlodawer A]] | ||
Current revision
Crystal structure of gp41-1 intein
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Categories: Large Structures | Metagenome | Beyer HM | Iwai H | Li M | Mikula KM | Wlodawer A
