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| - | [[Image:9dna.jpg|left|200px]] | |
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| - | {{Structure
| + | ==CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)== |
| - | |PDB= 9dna |SIZE=350|CAPTION= <scene name='initialview01'>9dna</scene>, resolution 1.800Å
| + | <StructureSection load='9dna' size='340' side='right'caption='[[9dna]], [[Resolution|resolution]] 1.80Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>
| + | <table><tr><td colspan='2'>[[9dna]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9DNA FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9dna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9dna OCA], [https://pdbe.org/9dna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9dna RCSB], [https://www.ebi.ac.uk/pdbsum/9dna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9dna ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=9dna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9dna OCA], [http://www.ebi.ac.uk/pdbsum/9dna PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=9dna RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Heinemann U]] |
| - | '''CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)'''
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| - | ==Overview==
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| - | Single crystals of the self-complementary octadeoxyribonucleotide d(GCCCGGGC) have been analysed by X-ray diffraction methods at a resolution of 1.8 A. The tetragonal unit cell of space group P4(3)2(1)2 has dimensions of a = 43.25 A and c = 24.61 A and contains eight strands of the oligonucleotide. The structure was refined by standard crystallographic techniques to an R factor of 17.1% using 1359 3 sigma structure factor observations. Two strands of the oligonucleotide are related by the crystallographic dyad axis to form a DNA helix in the A conformation. The d(GCCCGGGC) helix is characterized by a wide open major groove, a near perpendicular orientation of base pairs to the helix axis and an unusually small average helix twist angle of 31.3 degrees indicating a slightly underwound helix with 11.5 base pairs per turn. Extensive cross-strand stacking between guanine bases at the central cytosine-guanine step is made possible by a number of local conformational adjustments including a fully extended sugar-phosphate backbone of the central guanosine nucleotide.
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| - | ==About this Structure==
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| - | 9DNA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DNA OCA].
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| - | ==Reference==
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| - | Crystal structure analysis of an A-DNA fragment at 1.8 A resolution: d(GCCCGGGC)., Heinemann U, Lauble H, Frank R, Blocker H, Nucleic Acids Res. 1987 Nov 25;15(22):9531-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3684603 3684603]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Heinemann, U.]] | + | |
| - | [[Category: a-dna]]
| + | |
| - | [[Category: double helix]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:46:03 2008''
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