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| | ==Single particle analysis of Kir2.1NC_4 in negative stain== | | ==Single particle analysis of Kir2.1NC_4 in negative stain== |
| - | <StructureSection load='2xky' size='340' side='right' caption='[[2xky]], [[Resolution|resolution]] 17.20Å' scene=''> | + | <SX load='2xky' size='340' side='right' viewer='molstar' caption='[[2xky]], [[Resolution|resolution]] 17.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xky]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XKY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xky]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XKY FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gix|2gix]], [[1u4f|1u4f]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy , Hybrid , X-ray solution scattering, [[Resolution|Resolution]] 17.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xky OCA], [http://pdbe.org/2xky PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xky RCSB], [http://www.ebi.ac.uk/pdbsum/2xky PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xky ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xky OCA], [https://pdbe.org/2xky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xky RCSB], [https://www.ebi.ac.uk/pdbsum/2xky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xky ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IRK2_MOUSE IRK2_MOUSE]] Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. | + | [https://www.uniprot.org/uniprot/KCNJ2_MOUSE KCNJ2_MOUSE] Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Potassium Channel|Potassium Channel]] | + | *[[Potassium channel 3D structures|Potassium channel 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Collins, R F]] | + | [[Category: Mus musculus]] |
| - | [[Category: Fomina, S]] | + | [[Category: Collins RF]] |
| - | [[Category: Golovanova, M]] | + | [[Category: Fomina S]] |
| - | [[Category: Grossmann, J G]] | + | [[Category: Golovanova M]] |
| - | [[Category: Howard, T D]] | + | [[Category: Grossmann JG]] |
| - | [[Category: Leyland, M L]] | + | [[Category: Howard TD]] |
| - | [[Category: Prince, S M]]
| + | [[Category: Leyland ML]] |
| - | [[Category: Ryan, L O]] | + | [[Category: O'Ryan L]] |
| - | [[Category: Sleator, O K]] | + | [[Category: Prince SM]] |
| - | [[Category: Ion channel]] | + | [[Category: Sleator OK]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Metal transport]]
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| Structural highlights
Function
KCNJ2_MOUSE Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
Publication Abstract from PubMed
The interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using Transmission Electron Microscopy in negative stain. Three types of complexes were observed in electron micrographs corresponding to a 1:1 complex, a large self-enclosed tetrad complex and extended chains of linked channel domains. Using models derived from small angle X-ray scattering experiments in which high resolution structures from X-ray crystallographic and Nuclear Magnetic Resonance studies are positioned, the envelopes from single particle analysis can be resolved as a Kir2.1NC(4):PSD-95 complex and a tetrad of this unit (Kir2.1NC(4):PSD-95)(4). The tetrad complex shows the close association of the Kir2.1 cytoplasmic domains and the influence of PSD-95 mediated self-assembly on the clustering of these channels.
Self-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95.,Fomina S, Howard TD, Sleator OK, Golovanova M, O'Ryan L, Leyland ML, Grossmann JG, Collins RF, Prince SM Biochim Biophys Acta. 2011 Jul 5;1808(10):2374-2389. PMID:21756874[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Fomina S, Howard TD, Sleator OK, Golovanova M, O'Ryan L, Leyland ML, Grossmann JG, Collins RF, Prince SM. Self-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95. Biochim Biophys Acta. 2011 Jul 5;1808(10):2374-2389. PMID:21756874 doi:10.1016/j.bbamem.2011.06.021
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