2po4

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(New page: 200px {{Structure |PDB= 2po4 |SIZE=350|CAPTION= <scene name='initialview01'>2po4</scene>, resolution 2.000&Aring; |SITE= |LIGAND= |ACTIVITY= <span class='plainl...)
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[[Image:2po4.jpg|left|200px]]
 
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{{Structure
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==X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase==
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|PDB= 2po4 |SIZE=350|CAPTION= <scene name='initialview01'>2po4</scene>, resolution 2.000&Aring;
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<StructureSection load='2po4' size='340' side='right'caption='[[2po4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2po4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_N4 Escherichia virus N4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PO4 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE= gp50 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10752 Enterobacteria phage N4])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2po4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2po4 OCA], [https://pdbe.org/2po4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2po4 RCSB], [https://www.ebi.ac.uk/pdbsum/2po4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2po4 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2po4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2po4 OCA], [http://www.ebi.ac.uk/pdbsum/2po4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2po4 RCSB]</span>
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[https://www.uniprot.org/uniprot/RPOLV_BPN4 RPOLV_BPN4] DNA-dependent RNA polymerase, which is injected into the host upon infection and transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure.<ref>PMID:18362338</ref> <ref>PMID:19061645</ref>
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}}
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'''X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase'''
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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== References ==
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==Overview==
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<references/>
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Coliphage N4 virion RNA polymerase (vRNAP), which is injected into the host upon infection, transcribes the phage early genes from promoters that have a 5-bp stem-3 nt loop hairpin structure. Here, we describe the 2.0-A resolution x-ray crystal structure of N4 mini-vRNAP, a member of the T7-like, single-unit RNAP family and the minimal component having all RNAP functions of the full-length vRNAP. The structure resembles a "fisted right hand" with Fingers, Palm and Thumb subdomains connected to an N-terminal domain. We established that the specificity loop extending from the Fingers along with W129 of the N-terminal domain play critical roles in hairpin-promoter recognition. A comparison with the structure of the T7 RNAP initiation complex reveals that the pathway of the DNA to the active site is blocked in the apo-form vRNAP, indicating that vRNAP must undergo a large-scale conformational change upon promoter DNA binding and explaining the highly restricted promoter specificity of vRNAP that is essential for phage early transcription.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus N4]]
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2PO4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_n4 Enterobacteria phage n4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PO4 OCA].
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[[Category: Large Structures]]
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[[Category: Davydova EK]]
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==Reference==
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[[Category: Murakami KS]]
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X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase., Murakami KS, Davydova EK, Rothman-Denes LB, Proc Natl Acad Sci U S A. 2008 Mar 24;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18362338 18362338]
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[[Category: Rothman-Denes LB]]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Enterobacteria phage n4]]
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[[Category: Single protein]]
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[[Category: Davydova, E K.]]
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[[Category: Murakami, K S.]]
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[[Category: Rothman-Denes, L B.]]
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[[Category: right hand shape]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:57:51 2008''
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Current revision

X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase

PDB ID 2po4

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