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3qum

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==Crystal structure of human prostate specific antigen (PSA) in Fab sandwich with a high affinity and a PCa selective antibody==
==Crystal structure of human prostate specific antigen (PSA) in Fab sandwich with a high affinity and a PCa selective antibody==
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<StructureSection load='3qum' size='340' side='right' caption='[[3qum]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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<StructureSection load='3qum' size='340' side='right'caption='[[3qum]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3qum]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QUM FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3qum]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QUM FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zch|2zch]], [[2zck|2zck]], [[2zcl|2zcl]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900084:Thomsen-Friedenreich+antigen'>PRD_900084</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Semenogelase Semenogelase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.77 3.4.21.77] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qum OCA], [https://pdbe.org/3qum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qum RCSB], [https://www.ebi.ac.uk/pdbsum/3qum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qum ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qum OCA], [http://pdbe.org/3qum PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qum RCSB], [http://www.ebi.ac.uk/pdbsum/3qum PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qum ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/KLK3_HUMAN KLK3_HUMAN]] Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
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[https://www.uniprot.org/uniprot/KLK3_HUMAN KLK3_HUMAN] Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human prostate-specific antigen (PSA or human kallikrein-related peptidase 3) present in small quantities in the sera of healthy men becomes elevated in prostate cancer (PCa) and other prostate disorders. The ability to identify the free PSA fraction associated with PCa could increase the reliability of the PSA diagnostic test. Here we present the crystal structure of human PSA from seminal fluid in a sandwich complex with two monoclonal antibodies (mAbs). MAb 5D5A5 captures total PSA with exceptionally high affinity, and mAb 5D3D11 selectively discriminates between free PSA subforms that are more abundant in sera from patients with PCa. Although the antigen is not of seric origin, several insights into cancer diagnosis can be discerned from this complex. MAb 5D3D11 recognizes a PSA conformation different from that previously reported. Interacting with the kallikrein loop, the PSA N-linked glycan attached to asparagine 61 is an uncommonly complex sialated triantennary chain. O-Linked glycosylation is observed at threonine 125. The description of how PSA subforms in prostatic fluid can be discriminated using pairs of antibodies is a first step in the design of new strategies that are capable of real discrimination among PSA subforms, which will lead to the formulation of more reliable diagnostic tests. In a companion article [Muller, B. H., Savatier, A., L'Hostis, G., Costa, N., Bossus, M., Michel, S., et al. (2011). In vitro affinity maturation of an anti-PSA antibody for prostate cancer diagnostic assay. J. Mol. Biol.], we describe engineering efforts to improve the affinity of mAb 5D3D11, a first step towards such goal.
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Crystal Structure of Human Prostate-Specific Antigen in a Sandwich Antibody Complex.,Stura EA, Muller BH, Bossus M, Michel S, Jolivet-Reynaud C, Ducancel F J Mol Biol. 2011 Oct 20. PMID:22037582<ref>PMID:22037582</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3qum" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Semenogelase]]
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[[Category: Ducancel F]]
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[[Category: Ducancel, F]]
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[[Category: Michel S]]
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[[Category: Michel, S]]
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[[Category: Muller BH]]
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[[Category: Muller, B H]]
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[[Category: Stura EA]]
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[[Category: Stura, E A]]
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[[Category: Immune system]]
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[[Category: Kallikrein fold]]
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[[Category: N-linked and o-linked glycosylation]]
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[[Category: Natural post-transductional modification]]
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[[Category: Negative regulation of angiogenesis]]
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[[Category: Prostate-specific antigen]]
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[[Category: Serine protease]]
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Current revision

Crystal structure of human prostate specific antigen (PSA) in Fab sandwich with a high affinity and a PCa selective antibody

PDB ID 3qum

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