|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Solution structure of MTSL spin-labeled Schizosaccharomyces pombe Sin1 CRIM domain== | | ==Solution structure of MTSL spin-labeled Schizosaccharomyces pombe Sin1 CRIM domain== |
| - | <StructureSection load='2ruj' size='340' side='right' caption='[[2ruj]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2ruj' size='340' side='right'caption='[[2ruj]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ruj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RUJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RUJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ruj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RUJ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=R1A:3-{[(2,2,5,5-TETRAMETHYL-1-OXO-2,5-DIHYDRO-1H-PYRROLIUM-3-YL)METHYL]DISULFANYL}-D-ALANINE'>R1A</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sin1, SPAPYUG7.02c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=R1A:3-{[(2,2,5,5-TETRAMETHYL-1-OXO-2,5-DIHYDRO-1H-PYRROLIUM-3-YL)METHYL]DISULFANYL}-D-ALANINE'>R1A</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ruj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ruj OCA], [http://pdbe.org/2ruj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ruj RCSB], [http://www.ebi.ac.uk/pdbsum/2ruj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ruj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ruj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ruj OCA], [https://pdbe.org/2ruj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ruj RCSB], [https://www.ebi.ac.uk/pdbsum/2ruj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ruj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SIN1_SCHPO SIN1_SCHPO]] Interacts with the sty1 MAP kinase and has a role in the timing of the initiation of mitosis.<ref>PMID:10428959</ref> | + | [https://www.uniprot.org/uniprot/SIN1_SCHPO SIN1_SCHPO] Interacts with the sty1 MAP kinase and has a role in the timing of the initiation of mitosis.<ref>PMID:10428959</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fission yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Fujiwara, T]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Furuita, K]] | + | [[Category: Fujiwara T]] |
| - | [[Category: Ikegami, T]] | + | [[Category: Furuita K]] |
| - | [[Category: Kataoka, S]] | + | [[Category: Ikegami T]] |
| - | [[Category: Kobayashi, N]] | + | [[Category: Kataoka S]] |
| - | [[Category: Kojima, C]] | + | [[Category: Kobayashi N]] |
| - | [[Category: Shiozaki, K]] | + | [[Category: Kojima C]] |
| - | [[Category: Sugiki, T]] | + | [[Category: Shiozaki K]] |
| - | [[Category: Crim domain]]
| + | [[Category: Sugiki T]] |
| - | [[Category: Pre]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Sin1]]
| + | |
| - | [[Category: Torc2]]
| + | |
| Structural highlights
Function
SIN1_SCHPO Interacts with the sty1 MAP kinase and has a role in the timing of the initiation of mitosis.[1]
Publication Abstract from PubMed
NMR structure determination of soluble proteins depends in large part on distance restraints derived from NOE. In this study, we examined the impact of paramagnetic relaxation enhancement (PRE)-derived distance restraints on protein structure determination. A high-resolution structure of the loop-rich soluble protein Sin1 could not be determined by conventional NOE-based procedures due to an insufficient number of NOE restraints. By using the 867 PRE-derived distance restraints obtained from the NOE-based structure determination procedure, a high-resolution structure of Sin1 could be successfully determined. The convergence and accuracy of the determined structure were improved by increasing the number of PRE-derived distance restraints. This study demonstrates that PRE-derived distance restraints are useful in the determination of a high-resolution structure of a soluble protein when the number of NOE constraints is insufficient.
Utilization of paramagnetic relaxation enhancements for high-resolution NMR structure determination of a soluble loop-rich protein with sparse NOE distance restraints.,Furuita K, Kataoka S, Sugiki T, Hattori Y, Kobayashi N, Ikegami T, Shiozaki K, Fujiwara T, Kojima C J Biomol NMR. 2015 Jan;61(1):55-64. doi: 10.1007/s10858-014-9882-7. Epub 2014 Nov, 27. PMID:25428765[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wilkinson MG, Pino TS, Tournier S, Buck V, Martin H, Christiansen J, Wilkinson DG, Millar JB. Sin1: an evolutionarily conserved component of the eukaryotic SAPK pathway. EMBO J. 1999 Aug 2;18(15):4210-21. PMID:10428959 doi:http://dx.doi.org/10.1093/emboj/18.15.4210
- ↑ Furuita K, Kataoka S, Sugiki T, Hattori Y, Kobayashi N, Ikegami T, Shiozaki K, Fujiwara T, Kojima C. Utilization of paramagnetic relaxation enhancements for high-resolution NMR structure determination of a soluble loop-rich protein with sparse NOE distance restraints. J Biomol NMR. 2015 Jan;61(1):55-64. doi: 10.1007/s10858-014-9882-7. Epub 2014 Nov, 27. PMID:25428765 doi:http://dx.doi.org/10.1007/s10858-014-9882-7
|
