6qnh
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6qnh is ON HOLD Authors: Mehrabi, P., Schulz, E.C., Miller, R.J.D. Description: Liquid Application Method for time-resolved Analyses (LAMA) by seri...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 0ms timepoint== | |
| + | <StructureSection load='6qnh' size='340' side='right'caption='[[6qnh]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6qnh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_rubiginosus"_preobrazhenskaya_et_al._in_gauze_et_al._1957 "actinomyces rubiginosus" preobrazhenskaya et al. in gauze et al. 1957]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QNH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xylA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1929 "Actinomyces rubiginosus" Preobrazhenskaya et al. in Gauze et al. 1957])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qnh OCA], [http://pdbe.org/6qnh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qnh RCSB], [http://www.ebi.ac.uk/pdbsum/6qnh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qnh ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU]] Involved in D-xylose catabolism. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase. | ||
| - | + | Liquid application method for time-resolved analyses by serial synchrotron crystallography.,Mehrabi P, Schulz EC, Agthe M, Horrell S, Bourenkov G, von Stetten D, Leimkohl JP, Schikora H, Schneider TR, Pearson AR, Tellkamp F, Miller RJD Nat Methods. 2019 Oct;16(10):979-982. doi: 10.1038/s41592-019-0553-1. Epub 2019, Sep 16. PMID:31527838<ref>PMID:31527838</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6qnh" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Actinomyces rubiginosus preobrazhenskaya et al. in gauze et al. 1957]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Xylose isomerase]] | ||
[[Category: Mehrabi, P]] | [[Category: Mehrabi, P]] | ||
| - | [[Category: Miller, R | + | [[Category: Miller, R J.D]] |
| - | [[Category: Schulz, E | + | [[Category: Schulz, E C]] |
| + | [[Category: Complex]] | ||
| + | [[Category: Diffusion]] | ||
| + | [[Category: Dynamic]] | ||
| + | [[Category: Isomerase]] | ||
Current revision
Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 0ms timepoint
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