6qnz

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'''Unreleased structure'''
 
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The entry 6qnz is ON HOLD until Paper Publication
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==Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant==
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<StructureSection load='6qnz' size='340' side='right'caption='[[6qnz]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6qnz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._D6 Bacillus sp. D6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QNZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qnz OCA], [https://pdbe.org/6qnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qnz RCSB], [https://www.ebi.ac.uk/pdbsum/6qnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qnz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A3FEV7_9BACI A3FEV7_9BACI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The mutated nickase Nt.BspD6I E418A has been obtained by site-directed mutagenesis. The purified protein has been crystallized, and its spatial structure has been determined at 2.45 A resolution. An analysis of the crystal structures of the wild-type and mutated nickase have shown that the elimination of a carboxyl group due to the E418A mutation initiates marked conformational changes in both the N-terminal recognition domain and the C-terminal catalytic domain of nickase and insignificantly affects its linker domain. This is supported by changes in the functional properties of mutated nickase: an increase in the oligomerization capacity in the presence of a substrate, a reduction in the capacity to bind a substrate, and complete loss of catalytic activity.
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Authors: Artyukh, R.I., Kachalova, G.S., Yunusova, A.K., Gabdulkhakov, A.G., Fatkhullin, B.F., Atanasov, B.P., Perevyazova, T.A., Popov, A.N., Zheleznaya, L.A.
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The key role of E418 carboxyl group in the formation of Nt.BspD6I nickase active site: Structural and functional properties of Nt.BspD6I E418A mutant.,Artyukh RI, Kachalova GS, Yunusova AK, Fatkhullin BF, Atanasov BP, Perevyazova TA, Popov AN, Gabdulkhakov AG, Zheleznaya LA J Struct Biol. 2020 Apr 13:107508. doi: 10.1016/j.jsb.2020.107508. PMID:32298813<ref>PMID:32298813</ref>
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Description: Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Artyukh, R.I]]
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<div class="pdbe-citations 6qnz" style="background-color:#fffaf0;"></div>
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[[Category: Popov, A.N]]
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== References ==
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[[Category: Fatkhullin, B.F]]
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<references/>
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[[Category: Perevyazova, T.A]]
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__TOC__
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[[Category: Kachalova, G.S]]
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</StructureSection>
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[[Category: Zheleznaya, L.A]]
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[[Category: Bacillus sp. D6]]
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[[Category: Gabdulkhakov, A.G]]
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[[Category: Large Structures]]
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[[Category: Atanasov, B.P]]
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[[Category: Artyukh RI]]
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[[Category: Yunusova, A.K]]
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[[Category: Atanasov BP]]
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[[Category: Fatkhullin BF]]
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[[Category: Gabdulkhakov AG]]
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[[Category: Kachalova GS]]
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[[Category: Perevyazova TA]]
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[[Category: Popov AN]]
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[[Category: Yunusova AK]]
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[[Category: Zheleznaya LA]]

Current revision

Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant

PDB ID 6qnz

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