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| | ==Crystal structure of the nucleotide-free mutant A3B3== | | ==Crystal structure of the nucleotide-free mutant A3B3== |
| - | <StructureSection load='5zea' size='340' side='right' caption='[[5zea]], [[Resolution|resolution]] 3.38Å' scene=''> | + | <StructureSection load='5zea' size='340' side='right'caption='[[5zea]], [[Resolution|resolution]] 3.38Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zea]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Entha Entha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZEA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zea]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_hirae_ATCC_9790 Enterococcus hirae ATCC 9790]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZEA FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.384Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ntpA, EHR_08260 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=768486 ENTHA]), ntpB, EHR_08265 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=768486 ENTHA])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sodium-transporting_two-sector_ATPase Sodium-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.15 3.6.3.15] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zea OCA], [https://pdbe.org/5zea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zea RCSB], [https://www.ebi.ac.uk/pdbsum/5zea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zea ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zea OCA], [http://pdbe.org/5zea PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zea RCSB], [http://www.ebi.ac.uk/pdbsum/5zea PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zea ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NTPA_ENTHA NTPA_ENTHA]] Involved in ATP-driven sodium extrusion. [[http://www.uniprot.org/uniprot/NTPB_ENTHA NTPB_ENTHA]] Involved in ATP-driven sodium extrusion. | + | [https://www.uniprot.org/uniprot/NTPA_ENTHA NTPA_ENTHA] Involved in ATP-driven sodium extrusion. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5zea" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5zea" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[ATPase 3D structures|ATPase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Entha]] | + | [[Category: Enterococcus hirae ATCC 9790]] |
| - | [[Category: Sodium-transporting two-sector ATPase]] | + | [[Category: Large Structures]] |
| - | [[Category: Ichiro, Y]] | + | [[Category: Ichiro Y]] |
| - | [[Category: Imai, F L]] | + | [[Category: Imai FL]] |
| - | [[Category: Ishizuka-Katsura, Y]] | + | [[Category: Ishizuka-Katsura Y]] |
| - | [[Category: Maruyama, S]] | + | [[Category: Maruyama S]] |
| - | [[Category: Mizutani, K]] | + | [[Category: Mizutani K]] |
| - | [[Category: Murata, T]] | + | [[Category: Murata T]] |
| - | [[Category: Saito, Y]] | + | [[Category: Saito Y]] |
| - | [[Category: Shirouzu, M]] | + | [[Category: Shirouzu M]] |
| - | [[Category: Suzuki, K]] | + | [[Category: Suzuki K]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Rotary motor]]
| + | |
| - | [[Category: V-atpase]]
| + | |
| Structural highlights
Function
NTPA_ENTHA Involved in ATP-driven sodium extrusion.
Publication Abstract from PubMed
V1-ATPase is an ATP-driven rotary motor that is composed of a ring-shaped A3B3 complex and a central DF shaft. The nucleotide-free A3B3 complex of Enterococcus hirae, composed of three identical A1B1 heterodimers, showed a unique asymmetrical structure, probably due to the strong binding of the N-terminal barrel domain, which forms a crown structure. Here, we mutated the barrel region to weaken the crown, and performed structural analyses using high-speed atomic force microscopy and x-ray crystallography of the mutant A3B3. The nucleotide-free mutant A3B3 complex had a more symmetrical open structure than the wild type. Binding of nucleotides produced a closely packed spiral-like structure with a disrupted crown. These findings suggest that wild-type A3B3 forms a metastable (stressed) asymmetric structure composed of unstable A1B1 conformers due to the strong constraint of the crown. The results further the understanding of the principle of the cooperative transition mechanism of rotary motors.
Metastable asymmetrical structure of a shaftless V1 motor.,Maruyama S, Suzuki K, Imamura M, Sasaki H, Matsunami H, Mizutani K, Saito Y, Imai FL, Ishizuka-Katsura Y, Kimura-Someya T, Shirouzu M, Uchihashi T, Ando T, Yamato I, Murata T Sci Adv. 2019 Jan 30;5(1):eaau8149. doi: 10.1126/sciadv.aau8149. eCollection 2019, Jan. PMID:30729160[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Maruyama S, Suzuki K, Imamura M, Sasaki H, Matsunami H, Mizutani K, Saito Y, Imai FL, Ishizuka-Katsura Y, Kimura-Someya T, Shirouzu M, Uchihashi T, Ando T, Yamato I, Murata T. Metastable asymmetrical structure of a shaftless V1 motor. Sci Adv. 2019 Jan 30;5(1):eaau8149. doi: 10.1126/sciadv.aau8149. eCollection 2019, Jan. PMID:30729160 doi:http://dx.doi.org/10.1126/sciadv.aau8149
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