6dd9

From Proteopedia

(Difference between revisions)

Current revision

Structure of mouse SYCP3, P1 form

Structural highlights

6dd9 is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SYCP3_MOUSE Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase (PubMed:11311943, PubMed:22761579). Required for centromere pairing during meiosis in male germ cells (PubMed:22761579). Required for normal meiosis during spermatogenesis and male fertility (PubMed:10678170). Plays a lesser role in female fertility (PubMed:10678170, PubMed:12004129). Required for efficient phosphorylation of HORMAD1 and HORMAD2 (PubMed:22346761).^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

The meiotic chromosome axis plays key roles in meiotic chromosome organization and recombination, yet the underlying protein components of this structure are highly diverged. Here, we show that 'axis core proteins' from budding yeast (Red1), mammals (SYCP2/SYCP3), and plants (ASY3/ASY4) are evolutionarily related and play equivalent roles in chromosome axis assembly. We first identify 'closure motifs' in each complex that recruit meiotic HORMADs, the master regulators of meiotic recombination. We next find that axis core proteins form homotetrameric (Red1) or heterotetrameric (SYCP2:SYCP3 and ASY3:ASY4) coiled-coil assemblies that further oligomerize into micron-length filaments. Thus, the meiotic chromosome axis core in fungi, mammals, and plants shares a common molecular architecture, and likely also plays conserved roles in meiotic chromosome axis assembly and recombination control.

A conserved filamentous assembly underlies the structure of the meiotic chromosome axis.,West AM, Rosenberg SC, Ur SN, Lehmer MK, Ye Q, Hagemann G, Caballero I, Uson I, MacQueen AJ, Herzog F, Corbett KD Elife. 2019 Jan 18;8. pii: 40372. doi: 10.7554/eLife.40372. PMID:30657449^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yuan L, Liu JG, Zhao J, Brundell E, Daneholt B, Höög C. The murine SCP3 gene is required for synaptonemal complex assembly, chromosome synapsis, and male fertility. Mol Cell. 2000 Jan;5(1):73-83. PMID:10678170 doi:10.1016/s1097-2765(00)80404-9
↑ Botelho RJ, DiNicolo L, Tsao N, Karaiskakis A, Tarsounas M, Moens PB, Pearlman RE. The genomic structure of SYCP3, a meiosis-specific gene encoding a protein of the chromosome core. Biochim Biophys Acta. 2001 Apr 16;1518(3):294-9. PMID:11311943 doi:10.1016/s0167-4781(01)00171-3
↑ Yuan L, Liu JG, Hoja MR, Wilbertz J, Nordqvist K, Höög C. Female germ cell aneuploidy and embryo death in mice lacking the meiosis-specific protein SCP3. Science. 2002 May 10;296(5570):1115-8. PMID:12004129 doi:10.1126/science.1070594
↑ Fukuda T, Pratto F, Schimenti JC, Turner JM, Camerini-Otero RD, Höög C. Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis. PLoS Genet. 2012 Feb;8(2):e1002485. PMID:22346761 doi:10.1371/journal.pgen.1002485
↑ Bisig CG, Guiraldelli MF, Kouznetsova A, Scherthan H, Höög C, Dawson DS, Pezza RJ. Synaptonemal complex components persist at centromeres and are required for homologous centromere pairing in mouse spermatocytes. PLoS Genet. 2012 Jun;8(6):e1002701. PMID:22761579 doi:10.1371/journal.pgen.1002701
↑ West AM, Rosenberg SC, Ur SN, Lehmer MK, Ye Q, Hagemann G, Caballero I, Uson I, MacQueen AJ, Herzog F, Corbett KD. A conserved filamentous assembly underlies the structure of the meiotic chromosome axis. Elife. 2019 Jan 18;8. pii: 40372. doi: 10.7554/eLife.40372. PMID:30657449 doi:http://dx.doi.org/10.7554/eLife.40372

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6dd9"

@@ Line 1: / Line 1: @@
 ==Structure of mouse SYCP3, P1 form==
-<StructureSection load='6dd9' size='340' side='right' caption='[[6dd9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='6dd9' size='340' side='right'caption='[[6dd9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6dd9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DD9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6dd9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DD9 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6dd8|6dd8]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sycp3, mCG_16771 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dd9 OCA], [https://pdbe.org/6dd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dd9 RCSB], [https://www.ebi.ac.uk/pdbsum/6dd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dd9 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dd9 OCA], [http://pdbe.org/6dd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dd9 RCSB], [http://www.ebi.ac.uk/pdbsum/6dd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dd9 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SYCP3_MOUSE SYCP3_MOUSE] Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase (PubMed:11311943, PubMed:22761579). Required for centromere pairing during meiosis in male germ cells (PubMed:22761579). Required for normal meiosis during spermatogenesis and male fertility (PubMed:10678170). Plays a lesser role in female fertility (PubMed:10678170, PubMed:12004129). Required for efficient phosphorylation of HORMAD1 and HORMAD2 (PubMed:22346761).<ref>PMID:10678170</ref> <ref>PMID:11311943</ref> <ref>PMID:12004129</ref> <ref>PMID:22346761</ref> <ref>PMID:22761579</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Lk3 transgenic mice]]
+[[Category: Large Structures]]
-[[Category: Corbett, K D]]
+[[Category: Mus musculus]]
-[[Category: Munoz, I C]]
+[[Category: Corbett KD]]
-[[Category: Rosenberg, S C]]
+[[Category: Munoz IC]]
-[[Category: Uson, I]]
+[[Category: Rosenberg SC]]
-[[Category: Chromosome axis]]
+[[Category: Uson I]]
-[[Category: Coiled-coil]]
-[[Category: Meiosis]]
-[[Category: Structural protein]]

6dd9

From Proteopedia

Current revision

Structure of mouse SYCP3, P1 form

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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