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| | ==Structure of the Ysh1-Mpe1 nuclease complex from S.cerevisiae== | | ==Structure of the Ysh1-Mpe1 nuclease complex from S.cerevisiae== |
| - | <StructureSection load='6i1d' size='340' side='right' caption='[[6i1d]], [[Resolution|resolution]] 2.28Å' scene=''> | + | <StructureSection load='6i1d' size='340' side='right'caption='[[6i1d]], [[Resolution|resolution]] 2.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6i1d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I1D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6i1d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I1D FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.28Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YSH1, BRR5, YLR277C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), MPE1, YKL059C, YKL316 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i1d OCA], [http://pdbe.org/6i1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i1d RCSB], [http://www.ebi.ac.uk/pdbsum/6i1d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i1d ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i1d OCA], [https://pdbe.org/6i1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i1d RCSB], [https://www.ebi.ac.uk/pdbsum/6i1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i1d ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/YSH1_YEAST YSH1_YEAST]] Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Has endonuclease activity.<ref>PMID:15037765</ref> [[http://www.uniprot.org/uniprot/MPE1_YEAST MPE1_YEAST]] Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. | + | [https://www.uniprot.org/uniprot/YSH1_YEAST YSH1_YEAST] Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Has endonuclease activity.<ref>PMID:15037765</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Boreikaite, V]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Casanal, A]] | + | [[Category: Boreikaite V]] |
| - | [[Category: Degliesposti, G]] | + | [[Category: Casanal A]] |
| - | [[Category: Girbig, M]] | + | [[Category: Degliesposti G]] |
| - | [[Category: Hill, C H]] | + | [[Category: Girbig M]] |
| - | [[Category: Kubik, P]] | + | [[Category: Hill CH]] |
| - | [[Category: Kumar, A]] | + | [[Category: Kubik P]] |
| - | [[Category: Loeffelholz, O von]]
| + | [[Category: Kumar A]] |
| - | [[Category: Mariani, A]] | + | [[Category: Mariani A]] |
| - | [[Category: Maslen, S]] | + | [[Category: Maslen S]] |
| - | [[Category: Passmore, L A]] | + | [[Category: Passmore LA]] |
| - | [[Category: Skehel, M]] | + | [[Category: Skehel M]] |
| - | [[Category: 3' end]] | + | [[Category: Von Loeffelholz O]] |
| - | [[Category: Cleavage]]
| + | |
| - | [[Category: Cpf]]
| + | |
| - | [[Category: Endonuclease]]
| + | |
| - | [[Category: Gene regulation]]
| + | |
| - | [[Category: Metallo-beta-lactamase]]
| + | |
| - | [[Category: Mrna]]
| + | |
| - | [[Category: Nuclease]]
| + | |
| - | [[Category: Polya]]
| + | |
| - | [[Category: Polyadenylation]]
| + | |
| - | [[Category: Pre-mrna]]
| + | |
| Structural highlights
Function
YSH1_YEAST Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Has endonuclease activity.[1]
Publication Abstract from PubMed
Cleavage and polyadenylation factor (CPF/CPSF) is a multi-protein complex essential for formation of eukaryotic mRNA 3' ends. CPF cleaves pre-mRNAs at a specific site and adds a poly(A) tail. The cleavage reaction defines the 3' end of the mature mRNA, and thus the activity of the endonuclease is highly regulated. Here, we show that reconstitution of specific pre-mRNA cleavage with recombinant yeast proteins requires incorporation of the Ysh1 endonuclease into an eight-subunit "CPFcore" complex. Cleavage also requires the accessory cleavage factors IA and IB, which bind substrate pre-mRNAs and CPF, likely facilitating assembly of an active complex. Using X-ray crystallography, electron microscopy, and mass spectrometry, we determine the structure of Ysh1 bound to Mpe1 and the arrangement of subunits within CPFcore. Together, our data suggest that the active mRNA 3' end processing machinery is a dynamic assembly that is licensed to cleave only when all protein factors come together at the polyadenylation site.
Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex.,Hill CH, Boreikaite V, Kumar A, Casanal A, Kubik P, Degliesposti G, Maslen S, Mariani A, von Loeffelholz O, Girbig M, Skehel M, Passmore LA Mol Cell. 2019 Jan 31. pii: S1097-2765(18)31099-2. doi:, 10.1016/j.molcel.2018.12.023. PMID:30737185[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ryan K, Calvo O, Manley JL. Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease. RNA. 2004 Apr;10(4):565-73. PMID:15037765
- ↑ Hill CH, Boreikaite V, Kumar A, Casanal A, Kubik P, Degliesposti G, Maslen S, Mariani A, von Loeffelholz O, Girbig M, Skehel M, Passmore LA. Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex. Mol Cell. 2019 Jan 31. pii: S1097-2765(18)31099-2. doi:, 10.1016/j.molcel.2018.12.023. PMID:30737185 doi:http://dx.doi.org/10.1016/j.molcel.2018.12.023
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