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| | ==Crystal structure of RSP in complex with alpha-NAD+== | | ==Crystal structure of RSP in complex with alpha-NAD+== |
| - | <StructureSection load='3wgg' size='340' side='right' caption='[[3wgg]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='3wgg' size='340' side='right'caption='[[3wgg]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3wgg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_ethanolicus_jw_200 Thermoanaerobacter ethanolicus jw 200]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WGG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wgg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacter_ethanolicus_JW_200 Thermoanaerobacter ethanolicus JW 200]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WGG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8NA:ALPHA-DIPHOSPHOPYRIDINE+NUCLEOTIDE'>8NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wg9|3wg9]], [[3wgh|3wgh]], [[3wgi|3wgi]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8NA:ALPHA-DIPHOSPHOPYRIDINE+NUCLEOTIDE'>8NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rex, rsp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=509192 Thermoanaerobacter ethanolicus JW 200])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgg OCA], [https://pdbe.org/3wgg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wgg RCSB], [https://www.ebi.ac.uk/pdbsum/3wgg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wgg ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgg OCA], [http://pdbe.org/3wgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wgg RCSB], [http://www.ebi.ac.uk/pdbsum/3wgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wgg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/D5KM69_THEET D5KM69_THEET]] Modulates transcription in response to changes in cellular NADH/NAD(+) redox state (By similarity).[HAMAP-Rule:MF_01131][SAAS:SAAS003781_004_005573] | + | [https://www.uniprot.org/uniprot/D5KM69_THEET D5KM69_THEET] Modulates transcription in response to changes in cellular NADH/NAD(+) redox state (By similarity).[HAMAP-Rule:MF_01131][SAAS:SAAS003781_004_005573] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thermoanaerobacter ethanolicus jw 200]] | + | [[Category: Large Structures]] |
| - | [[Category: Guo, R T]] | + | [[Category: Thermoanaerobacter ethanolicus JW 200]] |
| - | [[Category: Ko, T P]] | + | [[Category: Guo R-T]] |
| - | [[Category: Zheng, Y]] | + | [[Category: Ko T-P]] |
| - | [[Category: Rossmann fold]]
| + | [[Category: Zheng Y]] |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription repressor]]
| + | |
| - | [[Category: Winged helix]]
| + | |
| Structural highlights
Function
D5KM69_THEET Modulates transcription in response to changes in cellular NADH/NAD(+) redox state (By similarity).[HAMAP-Rule:MF_01131][SAAS:SAAS003781_004_005573]
Publication Abstract from PubMed
The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). RSP is the homologue of Rex in Thermoanaerobacter ethanolicus JW200(T) and regulates ethanol fermentation in this obligate anaerobe. The dimeric repressor binds to DNA by an open conformation. The crystal structure of RSP/alpha-NAD(+) complex shows a different set of ligand interactions mainly due to the unique configuration of the nicotinamide moiety. The positively charged ring is covered by the Tyr102 side chain and interacts with a sulfate ion adjacent to the N-terminus of helix alpha8. Consequently, the RSP dimer may be locked in a closed conformation that does not bind to DNA. However, alpha-NAD(+) does not show a higher affinity to RSP than beta-NAD(+). It has to be improved for possible use as an effector in modulating the repressor.
Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor.,Zheng Y, Ko TP, Yang Y, Shao W, Guo RT Biochem Biophys Res Commun. 2015 Jan 16;456(3):733-6. doi:, 10.1016/j.bbrc.2014.12.049. Epub 2014 Dec 16. PMID:25527330[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zheng Y, Ko TP, Yang Y, Shao W, Guo RT. Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor. Biochem Biophys Res Commun. 2015 Jan 16;456(3):733-6. doi:, 10.1016/j.bbrc.2014.12.049. Epub 2014 Dec 16. PMID:25527330 doi:http://dx.doi.org/10.1016/j.bbrc.2014.12.049
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