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| | ==Structure of elastase inhibitor AFUEI (cyrstal form I)== | | ==Structure of elastase inhibitor AFUEI (cyrstal form I)== |
| - | <StructureSection load='3w0d' size='340' side='right' caption='[[3w0d]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='3w0d' size='340' side='right'caption='[[3w0d]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3w0d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus_af293 Aspergillus fumigatus af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W0D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W0D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3w0d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W0D FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w0e|3w0e]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w0d OCA], [http://pdbe.org/3w0d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3w0d RCSB], [http://www.ebi.ac.uk/pdbsum/3w0d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3w0d ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w0d OCA], [https://pdbe.org/3w0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w0d RCSB], [https://www.ebi.ac.uk/pdbsum/3w0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w0d ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IELA_ASPFU IELA_ASPFU]] Elastase inhibitor. Non-competitive inhibitor of A.fumigatus elastase with a Ki of 1.6 nM. Inhibits the fibrinogenase and collagenase activities of A.fumigatus elastase. Inhibitor of A.flavus elastase and human leukocyte elastase, and weakly inhibits porcine pancreatic elastase. Does not inhibit elastase from P.aeruginosa or C.atrox venom. | + | [https://www.uniprot.org/uniprot/IELA_ASPFU IELA_ASPFU] Elastase inhibitor. Non-competitive inhibitor of A.fumigatus elastase with a Ki of 1.6 nM. Inhibits the fibrinogenase and collagenase activities of A.fumigatus elastase. Inhibitor of A.flavus elastase and human leukocyte elastase, and weakly inhibits porcine pancreatic elastase. Does not inhibit elastase from P.aeruginosa or C.atrox venom. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aspergillus fumigatus af293]] | + | [[Category: Aspergillus fumigatus Af293]] |
| - | [[Category: Homma, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Imada, K]] | + | [[Category: Homma M]] |
| - | [[Category: Nikai, T]] | + | [[Category: Imada K]] |
| - | [[Category: Ogawa, K]] | + | [[Category: Nikai T]] |
| - | [[Category: Okumura, Y]] | + | [[Category: Ogawa K]] |
| - | [[Category: Sakuma, M]] | + | [[Category: Okumura Y]] |
| - | [[Category: Elastase inhibitor]]
| + | [[Category: Sakuma M]] |
| - | [[Category: Hydrolase inhibitor]]
| + | |
| - | [[Category: Secreted protein]]
| + | |
| Structural highlights
Function
IELA_ASPFU Elastase inhibitor. Non-competitive inhibitor of A.fumigatus elastase with a Ki of 1.6 nM. Inhibits the fibrinogenase and collagenase activities of A.fumigatus elastase. Inhibitor of A.flavus elastase and human leukocyte elastase, and weakly inhibits porcine pancreatic elastase. Does not inhibit elastase from P.aeruginosa or C.atrox venom.
Publication Abstract from PubMed
Elastase from Aspergillus sp. is an important factor for aspergillosis. AFUEI is an inhibitor of the elastase derived from Aspergillus fumigatus. AFUEI is a member of the I78 inhibitor family, and has a high inhibitory activity against elastases of Aspergillus fumigatus and Aspergillus flavus, human neutrophil elastase and bovine chymotrypsin, but does not inhibit bovine trypsin. Here we report the crystal structure of AFUEI in two crystal forms. AFUEI is a wedge-shaped protein composed of an extended loop and a scaffold protein core. The structure of AFUEI shows remarkable similarity to serine protease inhibitors of the potato inhibitor I family, although they are classified into different inhibitor families. A structural comparison with the potato I family inhibitors suggests that the extended loop of AFUEI corresponds to the binding loop of the potato inhibitor I family and AFUEI inhibits its cognate proteases through the same mechanism as the Potato I family inhibitors.
X-ray structure analysis and characterization of AFUEI, an elastase inhibitor from Aspergillus fumigatus.,Sakuma M, Imada K, Okumura Y, Uchiya KI, Yamashita N, Ogawa K, Hijikata A, Shirai T, Homma M, Nikai T J Biol Chem. 2013 May 2. PMID:23640894[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sakuma M, Imada K, Okumura Y, Uchiya KI, Yamashita N, Ogawa K, Hijikata A, Shirai T, Homma M, Nikai T. X-ray structure analysis and characterization of AFUEI, an elastase inhibitor from Aspergillus fumigatus. J Biol Chem. 2013 May 2. PMID:23640894 doi:10.1074/jbc.M112.433987
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