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3u5u

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Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity

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 ==Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity==
-<StructureSection load='3u5u' size='340' side='right' caption='[[3u5u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='3u5u' size='340' side='right'caption='[[3u5u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3u5u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ophioxylon_serpentinum Ophioxylon serpentinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U5U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U5U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3u5u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U5U FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3u57|3u57]], [[3u5y|3u5y]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Raucaffricine_beta-glucosidase Raucaffricine beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.125 3.2.1.125] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u5u OCA], [https://pdbe.org/3u5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u5u RCSB], [https://www.ebi.ac.uk/pdbsum/3u5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u5u ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u5u OCA], [http://pdbe.org/3u5u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3u5u RCSB], [http://www.ebi.ac.uk/pdbsum/3u5u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3u5u ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/RG1_RAUSE RG1_RAUSE] Glucosidase specifically involved in alkaloid biosynthesis leading to the accumulation of several alkaloids, including ajmaline, an important plant-derived pharmaceutical used in the treatment of heart disorders.<ref>PMID:10975500</ref>
-Two similar enzymes with different biosynthetic function in one species have evolved to catalyze two distinct reactions. X-ray structures of both enzymes help reveal their most important differences. The Rauvolfia alkaloid biosynthetic network harbors two O-glucosidases: raucaffricine glucosidase (RG), which hydrolyses raucaffricine to an intermediate downstream in the ajmaline pathway, and strictosidine glucosidase (SG), which operates upstream. RG converts strictosidine, the substrate of SG, but SG does not accept raucaffricine. Now elucidation of crystal structures of RG, inactive RG-E186Q mutant, and its complexes with ligands dihydro-raucaffricine and secologanin reveals that it is the "wider gate" of RG that allows strictosidine to enter the catalytic site, whereas the "slot-like" entrance of SG prohibits access by raucaffricine. Trp392 in RG and Trp388 in SG control the gate shape and acceptance of substrates. Ser390 directs the conformation of Trp392. 3D structures, supported by site-directed mutations and kinetic data of RG and SG, provide a structural and catalytic explanation of substrate specificity and deeper insights into O-glucosidase chemistry.
-Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity.,Xia L, Ruppert M, Wang M, Panjikar S, Lin H, Rajendran C, Barleben L, Stockigt J ACS Chem Biol. 2011 Oct 28. PMID:22004291<ref>PMID:22004291</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3u5u" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[Beta-glucosidase|Beta-glucosidase]]
+*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ophioxylon serpentinum]]
+[[Category: Large Structures]]
-[[Category: Raucaffricine beta-glucosidase]]
+[[Category: Rauvolfia serpentina]]
-[[Category: Barleben, L]]
+[[Category: Barleben L]]
-[[Category: Lin, H]]
+[[Category: Lin H]]
-[[Category: Panjikar, S]]
+[[Category: Panjikar S]]
-[[Category: Rajendran, C]]
+[[Category: Rajendran C]]
-[[Category: Ruppert, M]]
+[[Category: Ruppert M]]
-[[Category: Stoeckigt, J]]
+[[Category: Stoeckigt J]]
-[[Category: Wang, M]]
+[[Category: Wang M]]
-[[Category: Xia, L]]
+[[Category: Xia L]]
-[[Category: Hydrolase]]

3u5u

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Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity

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