6o0z

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'''Unreleased structure'''
 
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The entry 6o0z is ON HOLD
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==Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium==
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<SX load='6o0z' size='340' side='right' viewer='molstar' caption='[[6o0z]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6o0z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O0Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o0z OCA], [https://pdbe.org/6o0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o0z RCSB], [https://www.ebi.ac.uk/pdbsum/6o0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o0z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAS9_STRP1 CAS9_STRP1] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.<ref>PMID:21455174</ref> <ref>PMID:22745249</ref>
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Authors:
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==See Also==
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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Description:
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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__TOC__
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</SX>
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[[Category: Large Structures]]
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[[Category: Streptococcus pyogenes]]
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[[Category: Synthetic construct]]
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[[Category: Chittori S]]
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[[Category: Clarke R]]
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[[Category: Merk A]]
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[[Category: Merrill BJ]]
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[[Category: Puppala AK]]
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[[Category: Simonovic M]]
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[[Category: Subramaniam S]]
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[[Category: Zhu X]]

Current revision

Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium

6o0z, resolution 3.30Å

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OCA

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