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| | ==Structure of the SHQ1P-CBF5P complex== | | ==Structure of the SHQ1P-CBF5P complex== |
| - | <StructureSection load='3zv0' size='340' side='right' caption='[[3zv0]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='3zv0' size='340' side='right'caption='[[3zv0]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zv0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZV0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZV0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zv0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZV0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zuz|3zuz]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zv0 OCA], [http://pdbe.org/3zv0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zv0 RCSB], [http://www.ebi.ac.uk/pdbsum/3zv0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zv0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zv0 OCA], [https://pdbe.org/3zv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zv0 RCSB], [https://www.ebi.ac.uk/pdbsum/3zv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zv0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SHQ1_YEAST SHQ1_YEAST]] Involved in the early biogenesis steps of box H/ACA snoRNP assembly.<ref>PMID:12228251</ref> [[http://www.uniprot.org/uniprot/CBF5_YEAST CBF5_YEAST]] Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. May function as a pseudouridine synthase. Binds in vitro to centromeres and microtubules. It is a centromeric DNA-CBF3-binding factor which is involved in mitotic chromosome segregation. Essential for cell growth.<ref>PMID:8336724</ref> <ref>PMID:9315678</ref> <ref>PMID:9472021</ref> <ref>PMID:9848653</ref> <ref>PMID:10523634</ref> | + | [https://www.uniprot.org/uniprot/SHQ1_YEAST SHQ1_YEAST] Involved in the early biogenesis steps of box H/ACA snoRNP assembly.<ref>PMID:12228251</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Blaud, M]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Godin, K]] | + | [[Category: Blaud M]] |
| - | [[Category: Grozdanov, P N]] | + | [[Category: Godin K]] |
| - | [[Category: Leulliot, N]] | + | [[Category: Grozdanov PN]] |
| - | [[Category: Liger, D]] | + | [[Category: Leulliot N]] |
| - | [[Category: Machado-Pinilla, R]] | + | [[Category: Liger D]] |
| - | [[Category: Meier, U T]] | + | [[Category: Machado-Pinilla R]] |
| - | [[Category: Rety, S]] | + | [[Category: Meier UT]] |
| - | [[Category: Varani, G]] | + | [[Category: Rety S]] |
| - | [[Category: Walbott, H]] | + | [[Category: Varani G]] |
| - | [[Category: VanTilbeurgh, H]] | + | [[Category: Walbott H]] |
| - | [[Category: Cell cycle]]
| + | [[Category: VanTilbeurgh H]] |
| - | [[Category: Rnp assembly]]
| + | |
| - | [[Category: Telomerase]]
| + | |
| - | [[Category: X-linked dyskeratosis congenita]]
| + | |
| Structural highlights
Function
SHQ1_YEAST Involved in the early biogenesis steps of box H/ACA snoRNP assembly.[1]
Publication Abstract from PubMed
SHQ1 is an essential assembly factor for H/ACA ribonucleoproteins (RNPs) required for ribosome biogenesis, pre-mRNA splicing, and telomere maintenance. SHQ1 binds dyskerin/NAP57, the catalytic subunit of human H/ACA RNPs, and this interaction is modulated by mutations causing X-linked dyskeratosis congenita. We report the crystal structure of the C-terminal domain of yeast SHQ1, Shq1p, and its complex with yeast dyskerin/NAP57, Cbf5p, lacking its catalytic domain. The C-terminal domain of Shq1p interacts with the RNA-binding domain of Cbf5p and, through structural mimicry, uses the RNA-protein-binding sites to achieve a specific protein-protein interface. We propose that Shq1p operates as a Cbf5p chaperone during RNP assembly by acting as an RNA placeholder, thereby preventing Cbf5p from nonspecific RNA binding before association with an H/ACA RNA and the other core RNP proteins.
The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic.,Walbott H, Machado-Pinilla R, Liger D, Blaud M, Rety S, Grozdanov PN, Godin K, van Tilbeurgh H, Varani G, Meier UT, Leulliot N Genes Dev. 2011 Nov 15;25(22):2398-408. PMID:22085966[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yang PK, Rotondo G, Porras T, Legrain P, Chanfreau G. The Shq1p.Naf1p complex is required for box H/ACA small nucleolar ribonucleoprotein particle biogenesis. J Biol Chem. 2002 Nov 22;277(47):45235-42. Epub 2002 Sep 11. PMID:12228251 doi:10.1074/jbc.M207669200
- ↑ Walbott H, Machado-Pinilla R, Liger D, Blaud M, Rety S, Grozdanov PN, Godin K, van Tilbeurgh H, Varani G, Meier UT, Leulliot N. The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic. Genes Dev. 2011 Nov 15;25(22):2398-408. PMID:22085966 doi:10.1101/gad.176834.111
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