5yz4

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==Structure of the PIN domain endonuclease Utp24==
==Structure of the PIN domain endonuclease Utp24==
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<StructureSection load='5yz4' size='340' side='right' caption='[[5yz4]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
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<StructureSection load='5yz4' size='340' side='right'caption='[[5yz4]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5yz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YZ4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5yz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YZ4 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.135&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fcf1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yz4 OCA], [http://pdbe.org/5yz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yz4 RCSB], [http://www.ebi.ac.uk/pdbsum/5yz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yz4 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yz4 OCA], [https://pdbe.org/5yz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yz4 RCSB], [https://www.ebi.ac.uk/pdbsum/5yz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yz4 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/FCF1_SCHPO FCF1_SCHPO]] Essential protein involved in pre-rRNA processing and 40S ribosomal subunit assembly. Required for the early cleavage steps of 35S rRNA at the A(0), A(1), and A(2) sites (By similarity).
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[https://www.uniprot.org/uniprot/FCF1_SCHPO FCF1_SCHPO] Essential protein involved in pre-rRNA processing and 40S ribosomal subunit assembly. Required for the early cleavage steps of 35S rRNA at the A(0), A(1), and A(2) sites (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The precursor ribosomal RNA is processed by multiple steps of nucleolytic cleavage to generate mature rRNAs. Utp24 is a PIN domain endonuclease in the early 90S precursor of small ribosomal subunit and is proposed to cleave at sites A1 and A2 of pre-rRNA. Here we determine the crystal structure of Utp24 from Schizosaccharomyces pombe at 2.1 angstrom resolution. Utp24 structurally resembles the ribosome assembly factor Utp23 and both contain a Zn-finger motif. Functional analysis in Saccharomyces cerevisiae shows that depletion of Utp24 disturbs the assembly of 90S and abolishes cleavage at sites A0, A1 and A2. The 90S assembled with inactivated Utp24 is arrested at a post-A0-cleavage state and contains enriched nuclear exosome for degradation of 5' ETS. Despite of high sequence conservation, Utp24 from other organisms is unable to form an active 90S in S. cerevisiae, suggesting that Utp24 needs to be precisely positioned in 90S. Our study provides biochemical and structural insight into the role of Utp24 in 90S assembly and activity.
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Structural and functional analysis of Utp24, an endonuclease for processing 18S ribosomal RNA.,An W, Du Y, Ye K PLoS One. 2018 Apr 11;13(4):e0195723. doi: 10.1371/journal.pone.0195723., eCollection 2018. PMID:29641590<ref>PMID:29641590</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5yz4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Fission yeast]]
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[[Category: Large Structures]]
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[[Category: An, W]]
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[[Category: Schizosaccharomyces pombe 972h-]]
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[[Category: Du, Y]]
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[[Category: An W]]
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[[Category: Ye, K]]
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[[Category: Du Y]]
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[[Category: Hydrolase]]
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[[Category: Ye K]]
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[[Category: Pin domain]]
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[[Category: Ribonuclease]]
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Current revision

Structure of the PIN domain endonuclease Utp24

PDB ID 5yz4

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