This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


6nbu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:47, 13 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==CRISPR Complex Subunit Csm2 from Staphylococcus epidermidis RP62a==
==CRISPR Complex Subunit Csm2 from Staphylococcus epidermidis RP62a==
-
<StructureSection load='6nbu' size='340' side='right' caption='[[6nbu]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
+
<StructureSection load='6nbu' size='340' side='right'caption='[[6nbu]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[6nbu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staeq Staeq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NBU FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6nbu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis_RP62A Staphylococcus epidermidis RP62A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NBU FirstGlance]. <br>
-
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SERP2460 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176279 STAEQ])</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nbu OCA], [http://pdbe.org/6nbu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nbu RCSB], [http://www.ebi.ac.uk/pdbsum/6nbu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nbu ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nbu OCA], [https://pdbe.org/6nbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nbu RCSB], [https://www.ebi.ac.uk/pdbsum/6nbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nbu ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q5HK90_STAEQ Q5HK90_STAEQ]
-
Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated Cas proteins provide an immune-like response in many prokaryotes against extraneous nucleic acids. CRISPR-Cas systems are classified into different classes and types. Class 1 CRISPR-Cas systems form multi-protein effector complexes that includes a guide RNA (crRNA) used to identify the target for destruction. Here we present crystal structures of Staphylococcus epidermidis Type III-A CRISPR subunits Csm2 and Csm3 and a 5.2 A resolution single-particle cryo-electron microscopy (cryo-EM) reconstruction of an in vivo assembled effector subcomplex including the crRNA. The structures help to clarify the quaternary architecture of Type III-A effector complexes, and provide details on crRNA binding, target RNA binding and cleavage, and intermolecular interactions essential for effector complex assembly. The structures allow a better understanding of the organization of Type III-A CRISPR effector complexes as well as highlighting the overall similarities and differences with other Class 1 effector complexes.
+
-
 
+
-
Structural organization of a Type III-A CRISPR effector subcomplex determined by X-ray crystallography and cryo-EM.,Dorsey BW, Huang L, Mondragon A Nucleic Acids Res. 2019 Feb 13. pii: 5310821. doi: 10.1093/nar/gkz079. PMID:30759237<ref>PMID:30759237</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 6nbu" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Staeq]]
+
[[Category: Large Structures]]
-
[[Category: Dorsey, B W]]
+
[[Category: Staphylococcus epidermidis RP62A]]
-
[[Category: Huang, L]]
+
[[Category: Dorsey BW]]
-
[[Category: Mondragon, A]]
+
[[Category: Huang L]]
-
[[Category: Crispr]]
+
[[Category: Mondragon A]]
-
[[Category: Structural protein]]
+

Current revision

CRISPR Complex Subunit Csm2 from Staphylococcus epidermidis RP62a

PDB ID 6nbu

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6nbu"
Personal tools