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3zwe

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Structure of BambL, a lectin from Burkholderia ambifaria, complexed with blood group B epitope

Structural highlights

3zwe is a 3 chain structure with sequence from Burkholderia ambifaria AMMD. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.75Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q0B4G1_BURCM

Publication Abstract from PubMed

Burkholderia ambifaria is generally associated with the rhizosphere of plants where it has biocontrol effects on other microorganisms. It is also a member of the Burkholderia cepacia complex, a group of closely related bacteria that cause lung infections in immunocompromised patients as well as in patients with granulomatous disease or cystic fibrosis. Our previous work indicated that fucose on human epithelia is a frequent target for lectins and adhesins of lung pathogens (Sulak, O., Cioci, G., Lameignere, E., Balloy, V., Round, A., Gutsche, I., Malinovska, L., Chignard, M., Kosma, P., Aubert, D. F., Marolda, C. L., Valvano, M. A., Wimmerova, M., and Imberty, A. (2011) PLoS Pathog. 7, e1002238). Analysis of the B. ambifaria genome identified BambL as a putative fucose-binding lectin. The 87-amino acid protein was produced recombinantly and demonstrated to bind to fucosylated oligosaccharides with a preference for alphaFuc1-2Gal epitopes. Crystal structures revealed that it associates as a trimer with two fucose-binding sites per monomer. The overall fold is a six-bladed beta-propeller formed by oligomerization as in the Ralstonia solanacearum lectin and not by sequential domains like the fungal fucose lectin from Aleuria aurantia. The affinity of BambL for small fucosylated glycans is very high as demonstrated by microcalorimetry (K(D) < 1 mum). Plant cell wall oligosaccharides and human histo-blood group oligosaccharides H-type 2 and Lewis Y are bound with equivalent efficiency. Binding to artificial glycosphingolipid-containing vesicles, human saliva, and lung tissues confirmed that BambL could recognize a wide spectrum of fucosylated epitopes, albeit with a lower affinity for biological material from nonsecretor individuals.

Fucose-binding Lectin from Opportunistic Pathogen Burkholderia ambifaria Binds to Both Plant and Human Oligosaccharidic Epitopes.,Audfray A, Claudinon J, Abounit S, Ruvoen-Clouet N, Larson G, Smith DF, Wimmerova M, Le Pendu J, Romer W, Varrot A, Imberty A J Biol Chem. 2012 Feb 3;287(6):4335-47. Epub 2011 Dec 14. PMID:22170069^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Audfray A, Claudinon J, Abounit S, Ruvoen-Clouet N, Larson G, Smith DF, Wimmerova M, Le Pendu J, Romer W, Varrot A, Imberty A. Fucose-binding Lectin from Opportunistic Pathogen Burkholderia ambifaria Binds to Both Plant and Human Oligosaccharidic Epitopes. J Biol Chem. 2012 Feb 3;287(6):4335-47. Epub 2011 Dec 14. PMID:22170069 doi:10.1074/jbc.M111.314831

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zwe"

@@ Line 1: / Line 1: @@
 ==Structure of BambL, a lectin from Burkholderia ambifaria, complexed with blood group B epitope==
-<StructureSection load='3zwe' size='340' side='right' caption='[[3zwe]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
+<StructureSection load='3zwe' size='340' side='right'caption='[[3zwe]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zwe]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Burcm Burcm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZWE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZWE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zwe]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_ambifaria_AMMD Burkholderia ambifaria AMMD]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZWE FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zw2|3zw2]], [[3zw0|3zw0]], [[3zzv|3zzv]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zwe OCA], [http://pdbe.org/3zwe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zwe RCSB], [http://www.ebi.ac.uk/pdbsum/3zwe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zwe ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zwe OCA], [https://pdbe.org/3zwe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zwe RCSB], [https://www.ebi.ac.uk/pdbsum/3zwe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zwe ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q0B4G1_BURCM Q0B4G1_BURCM]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Burcm]]
+[[Category: Burkholderia ambifaria AMMD]]
-[[Category: Abounit, S]]
+[[Category: Large Structures]]
-[[Category: Audfray, A]]
+[[Category: Abounit S]]
-[[Category: Claudinon, J]]
+[[Category: Audfray A]]
-[[Category: Imberty, A]]
+[[Category: Claudinon J]]
-[[Category: Larson, G]]
+[[Category: Imberty A]]
-[[Category: LePendu, J]]
+[[Category: Larson G]]
-[[Category: Romer, W]]
+[[Category: LePendu J]]
-[[Category: Ruvoen-Clouet, N]]
+[[Category: Romer W]]
-[[Category: Varrot, A]]
+[[Category: Ruvoen-Clouet N]]
-[[Category: Wimmerova, M]]
+[[Category: Varrot A]]
-[[Category: B-propeller]]
+[[Category: Wimmerova M]]
-[[Category: Cystic fibrosis]]
-[[Category: Human histo-blood group]]
-[[Category: Sugar binding protein]]

3zwe

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Current revision

Structure of BambL, a lectin from Burkholderia ambifaria, complexed with blood group B epitope

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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