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| | ==NMR Structure of human RTF1 PLUS3 domain.== | | ==NMR Structure of human RTF1 PLUS3 domain.== |
| - | <StructureSection load='2bze' size='340' side='right' caption='[[2bze]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''> | + | <StructureSection load='2bze' size='340' side='right'caption='[[2bze]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2bze]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BZE FirstGlance]. <br> | + | <table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BZE FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bze OCA], [http://pdbe.org/2bze PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bze RCSB], [http://www.ebi.ac.uk/pdbsum/2bze PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bze ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bze OCA], [https://pdbe.org/2bze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bze RCSB], [https://www.ebi.ac.uk/pdbsum/2bze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bze ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Large Structures]] |
| - | [[Category: Ab, E]] | + | [[Category: Ab E]] |
| - | [[Category: Daniels, M A]] | + | [[Category: Daniels MA]] |
| - | [[Category: Diercks, T]] | + | [[Category: De Jong RN]] |
| - | [[Category: Folkers, G E]] | + | [[Category: Diercks T]] |
| - | [[Category: Jong, R N.De]] | + | [[Category: Folkers GE]] |
| - | [[Category: Kaptein, R]] | + | [[Category: Kaptein R]] |
| - | [[Category: SPINE, Structural Proteomics in Europe]]
| + | [[Category: Truffault V]] |
| - | [[Category: Truffault, V]] | + | |
| - | [[Category: Cdc73]]
| + | |
| - | [[Category: Ctr9]]
| + | |
| - | [[Category: Elongation]]
| + | |
| - | [[Category: H2b ubiquitination]]
| + | |
| - | [[Category: Histone h3 methylation]]
| + | |
| - | [[Category: Human rtf1 plus3 domain]]
| + | |
| - | [[Category: Leo1]]
| + | |
| - | [[Category: Paf1 complex]]
| + | |
| - | [[Category: Plus3 domain]]
| + | |
| - | [[Category: Spine]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Structural proteomics in europe]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The yeast Paf1 complex consists of Paf1, Rtf1, Cdc73, Ctr9, and Leo1 and regulates histone H2B ubiquitination, histone H3 methylation, RNA polymerase II carboxy-terminal domain (CTD) Ser2 phosphorylation, and RNA 3' end processing. We provide structural insight into the Paf1 complex with the NMR structure of the conserved and functionally important Plus3 domain of human Rtf1. A predominantly beta-stranded subdomain displays structural similarity to Dicer/Argonaute PAZ domains and to Tudor domains. We further demonstrate that the highly basic Rtf1 Plus3 domain can interact in vitro with single-stranded DNA via residues on the rim of the beta sheet, reminiscent of siRNA binding by PAZ domains, but did not detect binding to double-stranded DNA or RNA. We discuss the potential role of Rtf1 Plus3 ssDNA binding during transcription elongation.
Structure and DNA binding of the human Rtf1 Plus3 domain.,de Jong RN, Truffault V, Diercks T, Ab E, Daniels MA, Kaptein R, Folkers GE Structure. 2008 Jan;16(1):149-59. PMID:18184592[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ de Jong RN, Truffault V, Diercks T, Ab E, Daniels MA, Kaptein R, Folkers GE. Structure and DNA binding of the human Rtf1 Plus3 domain. Structure. 2008 Jan;16(1):149-59. PMID:18184592 doi:10.1016/j.str.2007.10.018
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