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| | ==Structure of BsUDG== | | ==Structure of BsUDG== |
| - | <StructureSection load='3zor' size='340' side='right' caption='[[3zor]], [[Resolution|resolution]] 2.95Å' scene=''> | + | <StructureSection load='3zor' size='340' side='right'caption='[[3zor]], [[Resolution|resolution]] 2.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zor]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZOR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zor]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZOR FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zoq|3zoq]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uracil-DNA_glycosylase Uracil-DNA glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.27 3.2.2.27] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zor OCA], [https://pdbe.org/3zor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zor RCSB], [https://www.ebi.ac.uk/pdbsum/3zor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zor ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zor OCA], [http://pdbe.org/3zor PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zor RCSB], [http://www.ebi.ac.uk/pdbsum/3zor PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zor ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UNG_BACSU UNG_BACSU]] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity). | + | [https://www.uniprot.org/uniprot/UNG_BACSU UNG_BACSU] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[DNA glycosylase|DNA glycosylase]] | + | *[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacsu]] | + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] |
| - | [[Category: Uracil-DNA glycosylase]] | + | [[Category: Large Structures]] |
| - | [[Category: Banos-Sanz, J I]] | + | [[Category: Banos-Sanz JI]] |
| - | [[Category: Gonzalez, B]] | + | [[Category: Gonzalez B]] |
| - | [[Category: Mojardin, L]] | + | [[Category: Mojardin L]] |
| - | [[Category: Salas, M]] | + | [[Category: Salas M]] |
| - | [[Category: Sanz-Aparicio, J]] | + | [[Category: Sanz-Aparicio J]] |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
UNG_BACSU Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity).
Publication Abstract from PubMed
Uracil-DNA glycosylase (UDG) is a key repair enzyme responsible for removing uracil residues from DNA. Interestingly, UDG is the only enzyme known to be inhibited by two different DNA mimic proteins: p56 encoded by the Bacillus subtilis phage 29 and the well-characterized protein Ugi encoded by the B. subtilis phage PBS1/PBS2. Atomic-resolution crystal structures of the B. subtilis UDG both free and in complex with p56, combined with site-directed mutagenesis analysis, allowed us to identify the key amino acid residues required for enzyme activity, DNA binding and complex formation. An important requirement for complex formation is the recognition carried out by p56 of the protruding Phe191 residue from B. subtilis UDG, whose side-chain is inserted into the DNA minor groove to replace the flipped-out uracil. A comparative analysis of both p56 and Ugi inhibitors enabled us to identify their common and distinctive features. Thereby, our results provide an insight into how two DNA mimic proteins with different structural and biochemical properties are able to specifically block the DNA-binding domain of the same enzyme.
Crystal structure and functional insights into uracil-DNA glycosylase inhibition by phage 29 DNA mimic protein p56.,Banos-Sanz JI, Mojardin L, Sanz-Aparicio J, Lazaro JM, Villar L, Serrano-Heras G, Gonzalez B, Salas M Nucleic Acids Res. 2013 May 13. PMID:23671337[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Banos-Sanz JI, Mojardin L, Sanz-Aparicio J, Lazaro JM, Villar L, Serrano-Heras G, Gonzalez B, Salas M. Crystal structure and functional insights into uracil-DNA glycosylase inhibition by phage 29 DNA mimic protein p56. Nucleic Acids Res. 2013 May 13. PMID:23671337 doi:10.1093/nar/gkt395
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