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| | ==Crystal structure of galactitol-1-phosphate dehydrogenase from Escherichia coli== | | ==Crystal structure of galactitol-1-phosphate dehydrogenase from Escherichia coli== |
| - | <StructureSection load='4a2c' size='340' side='right' caption='[[4a2c]], [[Resolution|resolution]] 1.87Å' scene=''> | + | <StructureSection load='4a2c' size='340' side='right'caption='[[4a2c]], [[Resolution|resolution]] 1.87Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4a2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A2C FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4a2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A2C FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactitol-1-phosphate_5-dehydrogenase Galactitol-1-phosphate 5-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.251 1.1.1.251] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a2c OCA], [http://pdbe.org/4a2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a2c RCSB], [http://www.ebi.ac.uk/pdbsum/4a2c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a2c ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a2c OCA], [https://pdbe.org/4a2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a2c RCSB], [https://www.ebi.ac.uk/pdbsum/4a2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a2c ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GATD_ECOLI GATD_ECOLI]] Converts galactitol 1-phosphate to tagatose 6-phosphate. | + | [https://www.uniprot.org/uniprot/GATD_ECOLI GATD_ECOLI] Converts galactitol 1-phosphate to tagatose 6-phosphate. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Galactitol-1-phosphate 5-dehydrogenase]] | + | [[Category: Large Structures]] |
| - | [[Category: Acebron, I]] | + | [[Category: Acebron I]] |
| - | [[Category: Alvarez, Y]] | + | [[Category: Alvarez Y]] |
| - | [[Category: Esteban-Torres, M]] | + | [[Category: Esteban-Torres M]] |
| - | [[Category: Mancheno, J M]] | + | [[Category: Mancheno JM]] |
| - | [[Category: Munoz, R]] | + | [[Category: Munoz R]] |
| - | [[Category: Rivas, B de las]] | + | [[Category: De las Rivas B]] |
| - | [[Category: Metal binding-site]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
GATD_ECOLI Converts galactitol 1-phosphate to tagatose 6-phosphate.
Publication Abstract from PubMed
Endogenous galactitol-1-phosphate 5-dehydrogenase (GPDH) (EC 1.1.1.251) from Escherichia coli spontaneously interacts with Ni(2+)-NTA matrices becoming a potential contaminant for recombinant, target His-tagged proteins. Purified recombinant, untagged GPDH (rGPDH) converted galactitol into tagatose, and d-tagatose-6-phosphate into galactitol-1-phosphate, in a Zn(2+)- and NAD(H)-dependent manner and readily crystallized what has permitted to solve its crystal structure. In contrast, N-terminally His-tagged GPDH was marginally stable and readily aggregated. The structure of rGPDH revealed metal-binding sites characteristic from the medium-chain dehydrogenase/reductase protein superfamily which may explain its ability to interact with immobilized metals. The structure also provides clues on the harmful effects of the N-terminal His-tag. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: GPDH and GPDHbind by molecular sieving (View interaction) GPDH and GPDHbind by x-ray crystallography(View interaction) GPDH and GPDHbind by cosedimentation in solution (View interaction).
The crystal structure of galactitol-1-phosphate 5-dehydrogenase from Escherichia coli K12 provides insights into its anomalous behavior on IMAC processes.,Esteban-Torres M, Alvarez Y, Acebron I, Rivas Bde L, Munoz R, Kohring GW, Roa AM, Sobrino M, Mancheno JM FEBS Lett. 2012 Sep 21;586(19):3127-33. doi: 10.1016/j.febslet.2012.07.073. Epub , 2012 Aug 8. PMID:22979983[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Esteban-Torres M, Alvarez Y, Acebron I, Rivas Bde L, Munoz R, Kohring GW, Roa AM, Sobrino M, Mancheno JM. The crystal structure of galactitol-1-phosphate 5-dehydrogenase from Escherichia coli K12 provides insights into its anomalous behavior on IMAC processes. FEBS Lett. 2012 Sep 21;586(19):3127-33. doi: 10.1016/j.febslet.2012.07.073. Epub , 2012 Aug 8. PMID:22979983 doi:http://dx.doi.org/10.1016/j.febslet.2012.07.073
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