Alkaline phosphatase
From Proteopedia
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'''Alkaline phosphatase''' (ALP) is an enzyme which removes phosphate from nucleotides, proteins and alkaloids. The enzyme is most effective in alkaline environment. Human ALP is present as 3 tissue-associated isozymes: intestinal, tissue-nonspecific and placental ('''ALPP'''). ALP is a zinc and magnesium containing enzyme. ALP is a glycoprotein which is attached by a GPI anchor to cell surfaces.<ref>PMID:15946677</ref> | '''Alkaline phosphatase''' (ALP) is an enzyme which removes phosphate from nucleotides, proteins and alkaloids. The enzyme is most effective in alkaline environment. Human ALP is present as 3 tissue-associated isozymes: intestinal, tissue-nonspecific and placental ('''ALPP'''). ALP is a zinc and magnesium containing enzyme. ALP is a glycoprotein which is attached by a GPI anchor to cell surfaces.<ref>PMID:15946677</ref> | ||
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| + | See [[Alkaline phosphatase (Hebrew)]] | ||
== Disease == | == Disease == | ||
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</StructureSection> | </StructureSection> | ||
| - | == 3D Structures of alkaline phosphatase == | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | *Alkaline phosphatase | ||
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| - | **[[1alk]], [[1ed9]], [[1ed8]] – EcALP + Zn + Mg – ''Eschechia coli''<br /> | ||
| - | **[[3bdh]] - EcALP + Mg<br /> | ||
| - | **[[1aja]], [[3bdf]], [[3bdg]] – EcALP (mutant) <br /> | ||
| - | **[[2mlx]], [[2mly]], [[2mlz]] – EcALP + trigger factor<br /> | ||
| - | **[[1ali]], [[1ajb]], [[1ajc]], [[1ajd]], [[1alj]], [[1ani]], [[1anj]], [[2anh]], [[1hqa]], [[1urb]], [[1hjk]], [[1khk]], [[1kh7]] - EcALP (mutant) + Zn + Mg<br /> | ||
| - | **[[1ura]], [[1khn]] , [[5tpq]] - EcALP (mutant) + Zn<br /> | ||
| - | **[[1b8j]] - EcALP + Zn + Mg + VO4<br /> | ||
| - | **[[5c66]] - EcALP + Zn + WO4<br /> | ||
| - | **[[1kh5]] - EcALP (mutant) + Zn + Mg + AlF3<BR /> | ||
| - | **[[1khj]] - EcALP (mutant) + Zn + AlF3<BR /> | ||
| - | **[[1k7h]] – nsALP + Zn + maleic acid – northern shrimp<br /> | ||
| - | **[[1shq]] - nsALP + Zn + Mg<br /> | ||
| - | **[[1zeb]] - hALPP + Zn + Mg – human<br /> | ||
| - | **[[1zef]] - hALPP + Zn + Mg + phenylalanine<br /> | ||
| - | **[[2glq]] - hALPP + Zn + Mg + Sr<br /> | ||
| - | **[[3e2d]] – ALP + Zn + Mg – ''Vibrio''<br /> | ||
| - | **[[3a52]] - ALP + Zn + Mg – ''Shewanella''<br /> | ||
| - | **[[3q3q]] - SpALP + Zn + Ca – ''Sphingomonas''<br /> | ||
| - | **[[2yeq]] - ALP D + Fe + Ca – ''Bacillus subtilis''<br /> | ||
| - | **[[3zwu]] - PfALP Phox + Fe2O + VO4 – ''Pseudomonas fluorescens''<br /> | ||
| - | **[[4a9v]] - PfALP Phox + Fe2O + Ca <br /> | ||
| - | **[[3wbh]] - ALP + Zn + Mg – ''Halomonas''<br /> | ||
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| - | *ALP complexes with phosphate derivative | ||
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| - | **[[1khl]], [[3dyc]], [[4km4]], [[4yr1]] - EcALP (mutant) + Zn + phosphate<br /> | ||
| - | **[[1alh]], [[3tg0]] - EcALP + Zn + phosphate<br /> | ||
| - | **[[1y6v]] - EcALP + Co + phosphate<br /> | ||
| - | **[[1y7a]] - EcALP (mutant) + Co + phosphate<br /> | ||
| - | **[[1elx]], [[1ely]], [[1elz]], [[1kh9]], [[1kh4]], [[2g9y]], [[3cmr]] - EcALP (mutant) + Zn + Mg + phosphate<br /> | ||
| - | **[[3dpc]] - EcALP (mutant) + phosphorylated peptide<br /> | ||
| - | **[[2ga3]] - EcALP (mutant) + Zn + Mg + phosphothreonine<br /> | ||
| - | **[[1ew8]] - EcALP + Zn + phosphonoacetic acid + phosphate<br /> | ||
| - | **[[1ew9]] - EcALP + Zn + Mg + mercaptomethyl phosphonate<br /> | ||
| - | **[[1shn]] - nsALP + Zn + phosphate<br /> | ||
| - | **[[1zed]] - hALPP + Zn + Mg + PO3 + nitrophenyl hydrogen methylphosphonate<br /> | ||
| - | **[[3mk0]], [[3mk1]] - hALPP + Zn + Mg + phosphate + nitrophenol + phosphoserine<br /> | ||
| - | **[[3mk2]] - hALPP + Zn + Mg + phosphoserine<br /> | ||
| - | **[[2iuc]] - AbALP + Zn + Mg + phosphate – ''Antarctic bacterium''<br /> | ||
| - | **[[2w5v]], [[2w5x]] - AbALP (mutant) + Zn + Mg + phosphoserine<br /> | ||
| - | **[[2w5w]] - AbALP (mutant) + Zn + phosphoserine<br /> | ||
| - | **[[2x98]] - ALP + Zn + Mg + phosphate – ''Halobacterium salinarum''<br /> | ||
| - | **[[4a9x]] - PfALP Phox + Fe2O + Ca + A[β,γ-methylene]TP <br /> | ||
| - | **[[4alf]] - PfALP Phox + Fe2O + Ca + phosphate <br /> | ||
| - | **[[4kjd]] - rALP + Mg + phosphoserine – rat<br /> | ||
| - | **[[4kjg]] - rALP + Zn + Mg + nitrophenyl phosphate <br /> | ||
| - | **[[5jk4]] - ALP + phosphate - ''Stenotrophomonas maltophilia'' <br /> | ||
| - | **[[5tj3]] - EmALP + Zn + phosphothreonine – ''Elizabethkingia meningoseptica''<br /> | ||
| - | **[[5xwi]] - SpALP + Zn + Ca + phosphothreonine<br /> | ||
| - | **[[5xwk]] - SpALP + Zn + Ca + phosphate<br /> | ||
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| - | *ALP complexes | ||
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| - | **[[2mlx]], [[2mly]], [[2mlz]] - EcALP residues 220-310 + trigger factor - NMR<br /> | ||
| - | **[[5jtl]] – EcALP + SecB<br /> | ||
| - | **[[5jtn]] – EcALP residues 91-145 + SecB<br /> | ||
| - | **[[5jto]] – EcALP residues 271-310 + SecB<br /> | ||
| - | **[[5too]] - EmALP (mutant) + Zn <br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Llinas P, Stura EA, Menez A, Kiss Z, Stigbrand T, Millan JL, Le Du MH. Structural studies of human placental alkaline phosphatase in complex with functional ligands. J Mol Biol. 2005 Jul 15;350(3):441-51. PMID:15946677 doi:http://dx.doi.org/10.1016/j.jmb.2005.04.068
- ↑ Stec B, Hehir MJ, Brennan C, Nolte M, Kantrowitz ER. Kinetic and X-ray structural studies of three mutant E. coli alkaline phosphatases: insights into the catalytic mechanism without the nucleophile Ser102. J Mol Biol. 1998 Apr 3;277(3):647-62. PMID:9533886 doi:10.1006/jmbi.1998.1635
