6jkj
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6jkj is ON HOLD Authors: Luo, Y., Kuang, Z. Description: Crystal structure of human SPSB2 in the apo-state Category: Unreleased Structures [[Ca...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of human SPSB2 in the apo-state== | |
| + | <StructureSection load='6jkj' size='340' side='right'caption='[[6jkj]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6jkj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JKJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jkj OCA], [https://pdbe.org/6jkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jkj RCSB], [https://www.ebi.ac.uk/pdbsum/6jkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jkj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SPSB2_HUMAN SPSB2_HUMAN] Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.<ref>PMID:15601820</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The SPRY domain-containing SOCS box protein 2 (SPSB2) is one of four mammalian SPSB proteins that are characterized by a C-terminal SOCS box and a central SPRY/B30.2 domain. SPSB2 interacts with inducible nitric oxide synthase (iNOS) via the SPRY domain and polyubiquitinates iNOS, resulting in its proteasomal degradation. Inhibitors that can disrupt SPSB2-iNOS interaction and augment NO production may serve as novel anti-infective and anticancer agents. The previously determined murine SPSB2 structure may not reflect the true apo conformation of the iNOS-binding site. Here, the crystal structure of human SPSB2 SPRY domain in the apo state is reported at a resolution of 1.9 A. Comparison of the apo and ligand-bound structures reveals that the iNOS-binding site is highly preformed and that major conformational changes do not occur upon ligand binding. Moreover, the C-terminal His6 tag of the recombinant protein binds to a shallow pocket adjacent to the iNOS-binding site on a crystallographically related SPSB2 molecule. These findings may help in structure-based and fragment-based SPSB2 inhibitor design in the future. | ||
| - | + | Crystal structure of the SPRY domain of human SPSB2 in the apo state.,Luo Y, Li K, Yang J, Zhang D, Zhou Y, Kuang Z Acta Crystallogr F Struct Biol Commun. 2019 Jun 1;75(Pt 6):412-418. doi:, 10.1107/S2053230X1900623X. Epub 2019 May 10. PMID:31204687<ref>PMID:31204687</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6jkj" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kuang | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kuang Z]] | ||
| + | [[Category: Luo Y]] | ||
Current revision
Crystal structure of human SPSB2 in the apo-state
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