1qoj
From Proteopedia
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==Crystal Structure of E.coli UvrB C-terminal domain, and a model for UvrB-UvrC interaction.== | ==Crystal Structure of E.coli UvrB C-terminal domain, and a model for UvrB-UvrC interaction.== | ||
| - | <StructureSection load='1qoj' size='340' side='right' | + | <StructureSection load='1qoj' size='340' side='right'caption='[[1qoj]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1qoj]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1qoj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QOJ FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qoj OCA], [https://pdbe.org/1qoj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qoj RCSB], [https://www.ebi.ac.uk/pdbsum/1qoj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qoj ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/UVRB_ECOLI UVRB_ECOLI] The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.<ref>PMID:12145219</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/1qoj_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/1qoj_consurf.spt"</scriptWhenChecked> | ||
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
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==See Also== | ==See Also== | ||
| - | *[[UvrABC|UvrABC]] | + | *[[UvrABC 3D structures|UvrABC 3D structures]] |
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| - | [[Category: Alexandrovich | + | [[Category: Large Structures]] |
| - | [[Category: Champness | + | [[Category: Alexandrovich A]] |
| - | [[Category: Fontecilla-Camps | + | [[Category: Champness J]] |
| - | [[Category: Goosen | + | [[Category: Fontecilla-Camps J]] |
| - | [[Category: Moolenaar | + | [[Category: Goosen N]] |
| - | [[Category: Sanderson | + | [[Category: Moolenaar G]] |
| - | [[Category: Sohi | + | [[Category: Sanderson MR]] |
| - | [[Category: Vernede | + | [[Category: Sohi M]] |
| - | [[Category: Visse | + | [[Category: Vernede X]] |
| - | + | [[Category: Visse R]] | |
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Current revision
Crystal Structure of E.coli UvrB C-terminal domain, and a model for UvrB-UvrC interaction.
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