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| | ==Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin== | | ==Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin== |
| - | <StructureSection load='6akf' size='340' side='right' caption='[[6akf]], [[Resolution|resolution]] 3.90Å' scene=''> | + | <StructureSection load='6akf' size='340' side='right'caption='[[6akf]], [[Resolution|resolution]] 3.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6akf]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AKF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6akf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AKF FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6ake|6ake]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cldn3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), cpe ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 "Bacillus perfringens" Veillon and Zuber 1898])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6akf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akf OCA], [https://pdbe.org/6akf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6akf RCSB], [https://www.ebi.ac.uk/pdbsum/6akf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6akf ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6akf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akf OCA], [http://pdbe.org/6akf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6akf RCSB], [http://www.ebi.ac.uk/pdbsum/6akf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6akf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CLD3_MOUSE CLD3_MOUSE]] Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.<ref>PMID:10508613</ref> [[http://www.uniprot.org/uniprot/ELTB_CLOPF ELTB_CLOPF]] This enterotoxin is responsible for many cases of a mild type of food poisoning. | + | [https://www.uniprot.org/uniprot/CLD3_MOUSE CLD3_MOUSE] Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.<ref>PMID:10508613</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus perfringens veillon and zuber 1898]] | + | [[Category: Clostridium perfringens]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Fujiyoshi, Y]] | + | [[Category: Mus musculus]] |
| - | [[Category: Irie, K]] | + | [[Category: Fujiyoshi Y]] |
| - | [[Category: Nakamura, S]] | + | [[Category: Irie K]] |
| - | [[Category: Cell adhesion]] | + | [[Category: Nakamura S]] |
| - | [[Category: Membrane protein-toxin complex]]
| + | |
| - | [[Category: Tight junction]]
| + | |
| Structural highlights
Function
CLD3_MOUSE Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.[1]
Publication Abstract from PubMed
Tight junction is a cell adhesion apparatus functioning as barrier and/or channel in the paracellular spaces of epithelia. Claudin is the major component of tight junction and polymerizes to form tight junction strands with various morphologies that may correlate with their functions. Here we present the crystal structure of mammalian claudin-3 at 3.6 A resolution. The third transmembrane helix of claudin-3 is clearly bent compared with that of other subtypes. Structural analysis of additional two mutants with a single mutation representing other subtypes in the third helix indicates that this helix takes a bent or straight structure depending on the residue. The presence or absence of the helix bending changes the positions of residues related to claudin-claudin interactions and affects the morphology and adhesiveness of the tight junction strands. These results evoke a model for tight junction strand formation with different morphologies - straight or curvy strands - observed in native epithelia.
Morphologic determinant of tight junctions revealed by claudin-3 structures.,Nakamura S, Irie K, Tanaka H, Nishikawa K, Suzuki H, Saitoh Y, Tamura A, Tsukita S, Fujiyoshi Y Nat Commun. 2019 Feb 18;10(1):816. doi: 10.1038/s41467-019-08760-7. PMID:30778075[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kubota K, Furuse M, Sasaki H, Sonoda N, Fujita K, Nagafuchi A, Tsukita S. Ca(2+)-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions. Curr Biol. 1999 Sep 23;9(18):1035-8. PMID:10508613
- ↑ Nakamura S, Irie K, Tanaka H, Nishikawa K, Suzuki H, Saitoh Y, Tamura A, Tsukita S, Fujiyoshi Y. Morphologic determinant of tight junctions revealed by claudin-3 structures. Nat Commun. 2019 Feb 18;10(1):816. doi: 10.1038/s41467-019-08760-7. PMID:30778075 doi:http://dx.doi.org/10.1038/s41467-019-08760-7
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