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| | ==Structure of mouse RECON (AKR1C13) in complex with cyclic AMP-AMP-GMP (cAAG)== | | ==Structure of mouse RECON (AKR1C13) in complex with cyclic AMP-AMP-GMP (cAAG)== |
| - | <StructureSection load='6m7k' size='340' side='right' caption='[[6m7k]], [[Resolution|resolution]] 1.10Å' scene=''> | + | <StructureSection load='6m7k' size='340' side='right'caption='[[6m7k]], [[Resolution|resolution]] 1.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6m7k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6M7K FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m7k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M7K FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Akr1c13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6m7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m7k OCA], [http://pdbe.org/6m7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m7k RCSB], [http://www.ebi.ac.uk/pdbsum/6m7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m7k ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m7k OCA], [https://pdbe.org/6m7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m7k RCSB], [https://www.ebi.ac.uk/pdbsum/6m7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m7k ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/AK1CD_MOUSE AK1CD_MOUSE]] Catalyzes the dehydrogenation of 17-beta-hydroxysteroids. May also exhibit significant activity with a variety of cyclic and alicyclic alcohols. Uses both NAD and NADP, but the activity is much greater with NAD than with NADP (By similarity). | + | [https://www.uniprot.org/uniprot/AK1CD_MOUSE AK1CD_MOUSE] Catalyzes the dehydrogenation of 17-beta-hydroxysteroids. May also exhibit significant activity with a variety of cyclic and alicyclic alcohols. Uses both NAD and NADP, but the activity is much greater with NAD than with NADP (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Eaglesham, J B]] | + | [[Category: Mus musculus]] |
| - | [[Category: King, D S]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Kranzusch, P J]] | + | [[Category: Eaglesham JB]] |
| - | [[Category: Lee, A S.Y]] | + | [[Category: King DS]] |
| - | [[Category: Mann, C C.de Oliveira]] | + | [[Category: Kranzusch PJ]] |
| - | [[Category: Mekalanos, J J]] | + | [[Category: Lee ASY]] |
| - | [[Category: Morehouse, B R]] | + | [[Category: Mekalanos JJ]] |
| - | [[Category: Nieminen, E A]] | + | [[Category: Morehouse BR]] |
| - | [[Category: Whiteley, A T]] | + | [[Category: Nieminen EA]] |
| - | [[Category: Akr1c13]] | + | [[Category: Whiteley AT]] |
| - | [[Category: Cyclic amp-amp-gmp]] | + | [[Category: De Oliveira Mann CC]] |
| - | [[Category: Innate immunity]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Recon]]
| + | |
| Structural highlights
Function
AK1CD_MOUSE Catalyzes the dehydrogenation of 17-beta-hydroxysteroids. May also exhibit significant activity with a variety of cyclic and alicyclic alcohols. Uses both NAD and NADP, but the activity is much greater with NAD than with NADP (By similarity).
Publication Abstract from PubMed
Cyclic dinucleotides (CDNs) have central roles in bacterial homeostasis and virulence by acting as nucleotide second messengers. Bacterial CDNs also elicit immune responses during infection when they are detected by pattern-recognition receptors in animal cells. Here we perform a systematic biochemical screen for bacterial signalling nucleotides and discover a large family of cGAS/DncV-like nucleotidyltransferases (CD-NTases) that use both purine and pyrimidine nucleotides to synthesize a diverse range of CDNs. A series of crystal structures establish CD-NTases as a structurally conserved family and reveal key contacts in the enzyme active-site lid that direct purine or pyrimidine selection. CD-NTase products are not restricted to CDNs and also include an unexpected class of cyclic trinucleotide compounds. Biochemical and cellular analyses of CD-NTase signalling nucleotides demonstrate that these cyclic di- and trinucleotides activate distinct host receptors and thus may modulate the interaction of both pathogens and commensal microbiota with their animal and plant hosts.
Bacterial cGAS-like enzymes synthesize diverse nucleotide signals.,Whiteley AT, Eaglesham JB, de Oliveira Mann CC, Morehouse BR, Lowey B, Nieminen EA, Danilchanka O, King DS, Lee ASY, Mekalanos JJ, Kranzusch PJ Nature. 2019 Feb 20. pii: 10.1038/s41586-019-0953-5. doi:, 10.1038/s41586-019-0953-5. PMID:30787435[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Whiteley AT, Eaglesham JB, de Oliveira Mann CC, Morehouse BR, Lowey B, Nieminen EA, Danilchanka O, King DS, Lee ASY, Mekalanos JJ, Kranzusch PJ. Bacterial cGAS-like enzymes synthesize diverse nucleotide signals. Nature. 2019 Feb 20. pii: 10.1038/s41586-019-0953-5. doi:, 10.1038/s41586-019-0953-5. PMID:30787435 doi:http://dx.doi.org/10.1038/s41586-019-0953-5
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