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4a82

From Proteopedia

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Current revision

Fitted model of staphylococcus aureus sav1866 model ABC transporter in the human cystic fibrosis transmembrane conductance regulator volume map EMD-1966.

Structural highlights

4a82 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron crystallography, Resolution 2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Y1866_STAAM May be involved in multidrug export. Transmembrane domains (TMD) form a pore in the cell membrane and the ATP-binding domain (NBD) is responsible for energy generation.

Publication Abstract from PubMed

Cystic fibrosis affects about 1 in 2500 live births and involves loss of transmembrane chloride flux due to a lack of a membrane protein channel termed the cystic fibrosis transmembrane conductance regulator (CFTR). We have studied CFTR structure by electron crystallography. The data were compared with existing structures of other ATP-binding cassette transporters. The protein was crystallized in the outward facing state and resembled the well characterized Sav1866 transporter. We identified regions in the CFTR map, not accounted for by Sav1866, which were potential locations for the regulatory region as well as the channel gate. In this analysis, we were aided by the fact that the unit cell was composed of two molecules not related by crystallographic symmetry. We also identified regions in the fitted Sav1866 model that were missing from the map, hence regions that were either disordered in CFTR or differently organized compared with Sav1866. Apart from the N and C termini, this indicated that in CFTR, the cytoplasmic end of transmembrane helix 5/11 and its associated loop could be partly disordered (or alternatively located).

The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR): THREE-DIMENSIONAL STRUCTURE AND LOCALIZATION OF A CHANNEL GATE.,Rosenberg MF, O'Ryan LP, Hughes G, Zhao Z, Aleksandrov LA, Riordan JR, Ford RC J Biol Chem. 2011 Dec 9;286(49):42647-54. Epub 2011 Sep 19. PMID:21931164^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rosenberg MF, O'Ryan LP, Hughes G, Zhao Z, Aleksandrov LA, Riordan JR, Ford RC. The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR): THREE-DIMENSIONAL STRUCTURE AND LOCALIZATION OF A CHANNEL GATE. J Biol Chem. 2011 Dec 9;286(49):42647-54. Epub 2011 Sep 19. PMID:21931164 doi:10.1074/jbc.M111.292268

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4a82"

@@ Line 3: / Line 3: @@
 <StructureSection load='4a82' size='340' side='right'caption='[[4a82]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4a82]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A82 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A82 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4a82]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A82 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A82 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a82 OCA], [http://pdbe.org/4a82 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a82 RCSB], [http://www.ebi.ac.uk/pdbsum/4a82 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a82 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a82 OCA], [https://pdbe.org/4a82 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a82 RCSB], [https://www.ebi.ac.uk/pdbsum/4a82 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a82 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Y1866_STAAM Y1866_STAAM] May be involved in multidrug export. Transmembrane domains (TMD) form a pore in the cell membrane and the ATP-binding domain (NBD) is responsible for energy generation.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Aleksandrov, L A]]
+[[Category: Large Structures]]
-[[Category: Ford, R C]]
+[[Category: Aleksandrov LA]]
-[[Category: Hughes, G]]
+[[Category: Ford RC]]
-[[Category: ORyan, L P]]
+[[Category: Hughes G]]
-[[Category: Riordan, J R]]
+[[Category: ORyan LP]]
-[[Category: Rosenberg, M F]]
+[[Category: Riordan JR]]
-[[Category: Zhao, Z]]
+[[Category: Rosenberg MF]]
-[[Category: Cassette protein]]
+[[Category: Zhao Z]]
-[[Category: Cftr]]
-[[Category: Cystic fibrosis]]
-[[Category: Ion channel]]
-[[Category: Transport protein]]

4a82

From Proteopedia

Current revision

Fitted model of staphylococcus aureus sav1866 model ABC transporter in the human cystic fibrosis transmembrane conductance regulator volume map EMD-1966.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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