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| | <StructureSection load='4ad8' size='340' side='right'caption='[[4ad8]], [[Resolution|resolution]] 4.00Å' scene=''> | | <StructureSection load='4ad8' size='340' side='right'caption='[[4ad8]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ad8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deira Deira]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AD8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AD8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ad8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AD8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AD8 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4abx|4abx]], [[4aby|4aby]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.998Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ad8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ad8 OCA], [https://pdbe.org/4ad8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ad8 RCSB], [https://www.ebi.ac.uk/pdbsum/4ad8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ad8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ad8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ad8 OCA], [http://pdbe.org/4ad8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ad8 RCSB], [http://www.ebi.ac.uk/pdbsum/4ad8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ad8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RECN_DEIRA RECN_DEIRA]] May be involved in recombinational repair of damaged DNA. | + | [https://www.uniprot.org/uniprot/RECN_DEIRA RECN_DEIRA] May be involved in recombinational repair of damaged DNA. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Adenosinetriphosphatase]] | + | [[Category: Deinococcus radiodurans R1]] |
| - | [[Category: Deira]] | + | [[Category: Large Structures]] |
| - | [[Category: McSweeney, S]] | + | [[Category: McSweeney S]] |
| - | [[Category: Pellegrino, S]] | + | [[Category: Pellegrino S]] |
| - | [[Category: Radzimanowski, J]] | + | [[Category: Radzimanowski J]] |
| - | [[Category: Sanctis, D de]]
| + | [[Category: Timmins J]] |
| - | [[Category: Timmins, J]] | + | [[Category: De Sanctis D]] |
| - | [[Category: Atpase domain]] | + | |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Dna repair]]
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| Structural highlights
Function
RECN_DEIRA May be involved in recombinational repair of damaged DNA.
Publication Abstract from PubMed
Repair of DNA double-strand breaks (DSBs) is essential for cell survival and maintaining genome integrity. DSBs are repaired in a stepwise manner by homologous recombination. Here, we focused on the early steps of DSB repair, including DSB recognition, which is still only poorly understood. In prokaryotes, this process has been proposed to involve the RecN protein, a member of the structural maintenance of chromosome (SMC) protein family, which include key eukaryotic and prokaryotic proteins such as cohesin, condensin, and Rad50. An extensive high- and low-resolution structural analysis of Deinococcus radiodurans RecN using a combination of protein crystallography and small-angle X-ray scattering enabled us to assemble a quasi-atomic model of the entire RecN protein, representing the complete structure of a SMC-like protein. These results, together with a thorough biochemical and mutational study of RecN, allow us to propose a model for the role of RecN in DSB repair.
Structural and Functional Characterization of an SMC-like Protein RecN: New Insights into Double-Strand Break Repair.,Pellegrino S, Radzimanowski J, de Sanctis D, Boeri Erba E, McSweeney S, Timmins J Structure. 2012 Dec 5;20(12):2076-89. doi: 10.1016/j.str.2012.09.010. Epub 2012, Oct 18. PMID:23085075[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pellegrino S, Radzimanowski J, de Sanctis D, Boeri Erba E, McSweeney S, Timmins J. Structural and Functional Characterization of an SMC-like Protein RecN: New Insights into Double-Strand Break Repair. Structure. 2012 Dec 5;20(12):2076-89. doi: 10.1016/j.str.2012.09.010. Epub 2012, Oct 18. PMID:23085075 doi:http://dx.doi.org/10.1016/j.str.2012.09.010
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