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| | <StructureSection load='4ayl' size='340' side='right'caption='[[4ayl]], [[Resolution|resolution]] 1.92Å' scene=''> | | <StructureSection load='4ayl' size='340' side='right'caption='[[4ayl]], [[Resolution|resolution]] 1.92Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ayl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacteroides_fragilis_subsp._ovatus"_(eggerth_and_gagnon_1933)_holdeman_and_moore_1970 "bacteroides fragilis subsp. ovatus" (eggerth and gagnon 1933) holdeman and moore 1970]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AYL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ayl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_ovatus Bacteroides ovatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AYL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.919Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ayj|4ayj]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycoprotein-fucosylgalactoside_alpha-N-acetylgalactosaminyltransferase Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.40 2.4.1.40] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ayl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ayl OCA], [https://pdbe.org/4ayl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ayl RCSB], [https://www.ebi.ac.uk/pdbsum/4ayl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ayl ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ayl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ayl OCA], [http://pdbe.org/4ayl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ayl RCSB], [http://www.ebi.ac.uk/pdbsum/4ayl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ayl ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A7LVT2_BACO1 A7LVT2_BACO1] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Glycosyltransferase|Glycosyltransferase]] | + | *[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase]] | + | [[Category: Bacteroides ovatus]] |
| - | [[Category: Acharya, K R]] | + | [[Category: Large Structures]] |
| - | [[Category: Brewb, K]] | + | [[Category: Acharya KR]] |
| - | [[Category: Lizotte-Waniewskib, M]] | + | [[Category: Brewb K]] |
| - | [[Category: Pham, T T.K]] | + | [[Category: Lizotte-Waniewskib M]] |
| - | [[Category: Stinsonb, B]] | + | [[Category: Pham TTK]] |
| - | [[Category: Sundriyala, A]] | + | [[Category: Stinsonb B]] |
| - | [[Category: Thiyagarajan, N]] | + | [[Category: Sundriyala A]] |
| - | [[Category: Tumbale, P]] | + | [[Category: Thiyagarajan N]] |
| - | [[Category: Histo-blood group enzyme]]
| + | [[Category: Tumbale P]] |
| - | [[Category: Transferase]]
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| Structural highlights
Function
A7LVT2_BACO1
Publication Abstract from PubMed
Histo-blood group antigens (HBGAs) are a source of antigenic variation between individuals that modulates resistance and susceptibility to pathogens and is a barrier to the spread of enveloped viruses. HBGAs are also produced by a few prokaryotes where they are synthesized by glycosyltransferases (GTs) related to human HBGA synthases. Here we report the first structure of a bacterial GT of this family, from an intestinal resident, Bacteroides ovatus. Unlike its mammalian homologues and other GTs with similar folds, this protein lacks a metal-binding Asp-X-Asp motif and is fully active in the absence of divalent metal ions, yet is strikingly similar in structure and in its interactions with substrates to structurally characterized mammalian metal-dependent mammalian homologues. This shows how an apparently major divergence in catalytic properties can be accommodated by minor structural adjustments and illustrates the structural underpinnings of horizontal transfer of a functional gene from prokaryotes to vertebrates.
Structure of a metal-independent bacterial glycosyltransferase that catalyzes the synthesis of histo-blood group A antigen.,Thiyagarajan N, Pham TT, Stinson B, Sundriyal A, Tumbale P, Lizotte-Waniewski M, Brew K, Acharya KR Sci Rep. 2012;2:940. doi: 10.1038/srep00940. Epub 2012 Dec 7. PMID:23230506[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Thiyagarajan N, Pham TT, Stinson B, Sundriyal A, Tumbale P, Lizotte-Waniewski M, Brew K, Acharya KR. Structure of a metal-independent bacterial glycosyltransferase that catalyzes the synthesis of histo-blood group A antigen. Sci Rep. 2012;2:940. doi: 10.1038/srep00940. Epub 2012 Dec 7. PMID:23230506 doi:http://dx.doi.org/10.1038/srep00940
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