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6jkw
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Seleno-methionine PNGM-1 from deep-sea sediment metagenome== | |
| + | <StructureSection load='6jkw' size='340' side='right'caption='[[6jkw]], [[Resolution|resolution]] 2.29Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6jkw]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JKW FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jkw OCA], [http://pdbe.org/6jkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jkw RCSB], [http://www.ebi.ac.uk/pdbsum/6jkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jkw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | OBJECTIVES: In order to find antimicrobial resistance gene(s) pre-dating the use of antibiotics through metagenomics, functional screening of a metagenomic library from the deep-seep sediments of Edison Seamount (ca. 10000 years old) was performed. METHODS: Among 60 antimicrobial-resistant clones, a single clone with the highest minimum inhibitory concentration (MIC) for ampicillin was selected. Sequence analysis revealed a new metallo-beta-lactamase (MBL) gene, designated as blaPNGM-1. PNGM-1 retains a zinc ion-binding motif (H116XH118XD120H121, H196 and H263), conserved in subclass B3 MBLs. The catalytic parameters of purified PNGM-1 and the MICs of beta-lactams for Escherichia coli TOP10 transformants harbouring the blaPNGM-1 gene were assessed. RESULTS: Antimicrobial susceptibility testing indicated reduced susceptibility to penicillins, narrow- and extended-spectrum cephalosporins, and carbapenems in E. coli TOP10 transformants harbouring the blaPNGM-1 gene. In addition, kinetic analyses revealed that PNGM-1 hydrolysed almost all beta-lactams. CONCLUSIONS: The PNGM-1 enzyme is the first case of a subclass B3 MBL derived from a functional metagenomic library of a deep-sea sediment that pre-dates the antibiotic era. | ||
| - | + | PNGM-1, a novel subclass B3 metallo-beta-lactamase from a deep-sea sediment metagenome.,Park KS, Kim TY, Kim JH, Lee JH, Jeon JH, Karim AM, Malik SK, Lee SH J Glob Antimicrob Resist. 2018 Sep;14:302-305. doi: 10.1016/j.jgar.2018.05.021., Epub 2018 May 26. PMID:29842976<ref>PMID:29842976</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Hong, M | + | <div class="pdbe-citations 6jkw" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Kang, L | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Lee, | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Park, Y | + | [[Category: Hong, M K]] |
| + | [[Category: Jeon, J H]] | ||
| + | [[Category: Kang, L W]] | ||
| + | [[Category: Lee, J H]] | ||
| + | [[Category: Lee, S H]] | ||
| + | [[Category: Park, K S]] | ||
| + | [[Category: Park, Y S]] | ||
| + | [[Category: Antibiotic resistance]] | ||
| + | [[Category: Mbl]] | ||
| + | [[Category: Metagenome]] | ||
| + | [[Category: Psi-biology]] | ||
| + | [[Category: Structural genomic]] | ||
| + | [[Category: Transferase]] | ||
| + | [[Category: Zinc binding motif]] | ||
Current revision
Seleno-methionine PNGM-1 from deep-sea sediment metagenome
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