6jl8
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6jl8 is ON HOLD Authors: Mase, H., Imamura, A., Nishimura, S., Inui, T. Description: Crystal structure of GMP reductase C318A from Trypanosoma bruc...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of GMP reductase C318A from Trypanosoma brucei== | |
| + | <StructureSection load='6jl8' size='340' side='right'caption='[[6jl8]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6jl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JL8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.804Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jl8 OCA], [https://pdbe.org/6jl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jl8 RCSB], [https://www.ebi.ac.uk/pdbsum/6jl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jl8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q57ZS7_TRYB2 Q57ZS7_TRYB2] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Guanosine 5'-monophosphate reductase (GMPR) is involved in the purine salvage pathway and is conserved throughout evolution. Nonetheless, the GMPR of Trypanosoma brucei (TbGMPR) includes a unique structure known as the cystathionine-beta-synthase (CBS) domain, though the role of this domain is not fully understood. Here, we show that guanine and adenine nucleotides exert positive and negative effects, respectively, on TbGMPR activity by binding allosterically to the CBS domain. The present structural analyses revealed that TbGMPR forms an octamer that shows a transition between relaxed and twisted conformations in the absence and presence of guanine nucleotides, respectively, whereas the TbGMPR octamer dissociates into two tetramers when ATP is available instead of guanine nucleotides. These findings demonstrate that the CBS domain plays a key role in the allosteric regulation of TbGMPR by facilitating the transition of its oligomeric state depending on ligand nucleotide availability. | ||
| - | + | Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-beta-synthase domain.,Imamura A, Okada T, Mase H, Otani T, Kobayashi T, Tamura M, Kubata BK, Inoue K, Rambo RP, Uchiyama S, Ishii K, Nishimura S, Inui T Nat Commun. 2020 Apr 15;11(1):1837. doi: 10.1038/s41467-020-15611-3. PMID:32296055<ref>PMID:32296055</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6jl8" style="background-color:#fffaf0;"></div> |
| - | [[Category: Imamura | + | == References == |
| - | [[Category: Mase | + | <references/> |
| - | [[Category: Nishimura | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Trypanosoma brucei brucei]] | ||
| + | [[Category: Imamura A]] | ||
| + | [[Category: Inui T]] | ||
| + | [[Category: Mase H]] | ||
| + | [[Category: Nishimura S]] | ||
Current revision
Crystal structure of GMP reductase C318A from Trypanosoma brucei
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